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. 2016 Mar 7;7(15):20597–20611. doi: 10.18632/oncotarget.7972

Figure 4. MEK1 mainly promotes SIRT1 expression in HCC population.

Figure 4

(A) Western blot analysis the sirtuins expression level in CSCs and non-CSCs, compared with U0126 inhibited CSCs. (B) Western blot analysis SIRT1 expression in CSCs after co-cultured with indicated concentration of U0126 or PD98059 (D) for 48 hours. (C) Western blot analysis SIRT1 expression in CSCs which cultured with 5 μM U0126 for indicated times. (E) Western blot analysis SIRT1 expression in CSCs transduced with lentiviruses expressing the indicated shRNA for 48 hours. Those experiments were repeated in two HCC cell lines (Huh7 and PLC/PRF/5).