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. 1985 Apr;38(4):399–402. doi: 10.1136/jcp.38.4.399

Investigation into the value of Papanicolaou stained cervical smears for the diagnosis of chlamydial cervical infection.

G E Forster, I Cookey, P E Munday, P I Richman, R Jha, D Coleman, B J Thomas, D A Hawkins, R T Evans, D Taylor-Robinson
PMCID: PMC499165  PMID: 2580863

Abstract

Forty five (37%) of 121 female contacts of men with non-gonococcal urethritis or gonorrhoea were chlamydia positive, as judged by isolation or by detecting elementary bodies in smears with a fluorescein labelled chlamydial monoclonal antibody. Only six (13%) of these, however, had Papanicolaou stained smears in which there were inclusion like changes suggestive of chlamydial infection. Furthermore, of 15 patients who had such cytological changes, chlamydiae were detected in only six and the abnormalities were found also in Papanicolaou stained smears from 10 (13%) of the 76 chlamydia negative patients. Modifying the Papanicolaou stained smears by including endocervical material did not increase sensitivity. In addition, destaining and restaining them with the monoclonal antibody was time consuming and the results were unreliable. The staining of cervical smears with Papanicolaou reagent is a technique of low sensitivity and specificity for diagnosing or screening for chlamydial cervical infection and cannot be recommended.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dorman S. A., Danos L. M., Wilson D. J., Noller K. L., Malkasian G. D., Goellner J. R., Smith T. F. Detection of chlamydial cervicitis by Papanicolaou stained smears and culture. Am J Clin Pathol. 1983 Apr;79(4):421–425. doi: 10.1093/ajcp/79.4.421. [DOI] [PubMed] [Google Scholar]
  2. Forster G., Jha R., Cheetham D., Munday P., Coleman D., Taylor-Robinson D. Cytological diagnosis of chlamydial infection of female genital tract. Lancet. 1983 Sep 3;2(8349):578–579. doi: 10.1016/s0140-6736(83)90613-x. [DOI] [PubMed] [Google Scholar]
  3. Giampaolo C., Murphy J., Benes S., McCormack W. M. How sensitive is the Papanicolaou smear in the diagnosis of infections with Chlamydia trachomatis? Am J Clin Pathol. 1983 Dec;80(6):844–849. doi: 10.1093/ajcp/80.6.844. [DOI] [PubMed] [Google Scholar]
  4. Gupta P. K., Lee E. F., Erozan Y. S., Frost J. K., Geddes S. T., Donovan P. A. Cytologic investigations in Chlamydia infection. Acta Cytol. 1979 Jul-Aug;23(4):315–320. [PubMed] [Google Scholar]
  5. Medley G. Cytological diagnosis of chlamydial infection of female genital tract. Lancet. 1983 Jun 25;1(8339):1449–1449. doi: 10.1016/s0140-6736(83)92399-1. [DOI] [PubMed] [Google Scholar]
  6. Mårdh P. A., Weström L., Colleen S., Wølner-Hanssen P. Sampling, specimen handling, and isolation techniques in the diagnosis of Chlamydial and other genital infections. Sex Transm Dis. 1981 Oct-Dec;8(4):280–285. doi: 10.1097/00007435-198110000-00007. [DOI] [PubMed] [Google Scholar]
  7. Naib Z. M. Cytology of TRIC agent infection of the eye of newborn infants and their mothers' genital tracts. Acta Cytol. 1970 Jul-Aug;14(7):390–395. [PubMed] [Google Scholar]
  8. Paavonen J., Purola E. Cytologic findings in cervical chlamydial infection. Med Biol. 1980 Jun;58(3):174–178. [PubMed] [Google Scholar]
  9. Purola E., Paavonen J. Routine cytology as a diagnostic aid in chlamydial cervicitis. Scand J Infect Dis Suppl. 1982;32:55–58. [PubMed] [Google Scholar]
  10. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]
  11. Thomas B. J., Evans R. T., Hawkins D. A., Taylor-Robinson D. Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation. J Clin Pathol. 1984 Jul;37(7):812–816. doi: 10.1136/jcp.37.7.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thomas B. J., Evans R. T., Hutchinson G. R., Taylor-Robinson D. Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining. J Clin Microbiol. 1977 Sep;6(3):285–292. doi: 10.1128/jcm.6.3.285-292.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. de Borges R. J., Carmona O., Machado H., Esparza J. Chlamydial infection in Papanicolaou-stained cervical smears. Acta Cytol. 1984 Jul-Aug;28(4):471–476. [PubMed] [Google Scholar]

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