Table 3. Y. pestis specific primers for standard end-point PCR and real-time PCR.
| Forward Primer | Sequence (5′–3′) | Reverse primer | Sequence (5′–3′) | Probe | Sequence (5′–3′) | Gene | Position |
|---|---|---|---|---|---|---|---|
| Y.pes/pPCP/8374/F | CCCGAAAGGAG TGCGGGTAA | Y.pes/pPCP/8902/R | CGCCCCGTCATT ATGGTGAA | N/A | N/A | pla | 8374–8902a |
| cafpri_f | CCAGCCCGCAT CACT | cafpri_r | ATCTGTAAAGTTAA CAGATGTGCTAGT | Tqpro_caf | JOE-AGCGTACCAA CAAGTAATTCTGTA TCGATG-BHQ1 | caf1 | 109–255b |
| Y.pes_pPCP_F | AGACATCCGG CTCACGTTAT | Y.pes_pPCP_R | GAGTACCTCCT TTGCCCTCA | Y.pes_pPCP_Pr | FAM-CACCTAA TGCCAAAGTCTTT GCGGA-TAMRA | pla | 8550–8669a |
Notes.
a
The nucleotide position of the Y.pes_pPCP_F and Y.pes_pPCP_R primers based on the CP000310.1 Yersinia pestis Antiqua plasmid pPCP.
b
The nucleotide position of the cafpri_f and cafpri_r primers based on the KF682424.1 Yersinia pestis strain S1 plasmid pMT1 capsule protein F1 (caf1) gene.