Abstract
Erythrocyte adenine nucleotide concentrations were measured in 154 patients with erythrocyte autoantibodies and 811 normal subjects using a luciferin-luciferase bioluminescent assay. The patients were initially divided into haemolysing and non-haemolysing groups. Red cell adenosine triphosphate (ATP) concentrations were significantly raised in the 96 patients with active haemolysis compared with the normal subjects and with the 58 patients in the non-haemolysing group. Although the patients categorised as non-haemolysing had higher ATP values than the normal subjects, this was because several patients had the anaemia of chronic disorders, which is associated with a shortened red cell life. Measurement of red cell ATP was a useful indicator of mean erythrocyte age and a raised value in patients with erythrocyte autoantibodies reflected active haemolysis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ABRAHAMOV A., DIAMOND L. K. Erythrocyte glycolysis in erythroblastotic newborns. Erythrocyte glycolysis also in Rh-positive red cells coated with anti-D antibodies. AMA J Dis Child. 1960 Feb;99:202–205. [PubMed] [Google Scholar]
- Cartwright G. E., Lee G. R. The anaemia of chronic disorders. Br J Haematol. 1971 Aug;21(2):147–152. doi: 10.1111/j.1365-2141.1971.tb03424.x. [DOI] [PubMed] [Google Scholar]
- GREENWALT T. J., MORELL S. A., AYERS V. E. PHOSPHATE PARTITION IN THE ERYTHROCYTES OF NORMAL NEWBORN INFANTS AND INFANTS WITH ERYTHROBLASTOSIS FETALIS. IV. ION EXCHANGE CHROMATOGRAPHY. Blood. 1963 Nov;22:589–599. [PubMed] [Google Scholar]
- Letarte J., Shahidi N. T., Hitzig W. H. L'activité de l'adénosine-triphosphatase chez les individus normaux et les malades atteints d'anémie hémolytique. Schweiz Med Wochenschr. 1965 Oct 23;95(43):1440–1442. [PubMed] [Google Scholar]
- Lichtman M. A., Miller D. R., Weed R. I. Energy metabolism in uremic red cells: relationship of red cell adenosine triphosphate concentration to extracellular phosphate. Trans Assoc Am Physicians. 1969;82:331–343. [PubMed] [Google Scholar]
- Olsson T., Gulliksson H., Palmeborn M., Bergström K., Thore A. Methodological aspects on the firefly luciferase assay of adenine nucleotides in whole blood and red blood cells. Scand J Clin Lab Invest. 1983 Dec;43(8):657–664. [PubMed] [Google Scholar]
- Palek J., Mircevová L., Brabec V., Friedmann B., Májský A. The effect of anti A antibody on red cell organic phosphates and adenosine triphosphatase activity in vitro. Scand J Haematol. 1968;5(3):191–210. doi: 10.1111/j.1600-0609.1968.tb01737.x. [DOI] [PubMed] [Google Scholar]
- STORTI E., VACCARI F., BALDINI E. Changes in red cells metabolism in presence of incomplete antibodies. Experientia. 1956 Mar 15;12(3):108–109. doi: 10.1007/BF02157180. [DOI] [PubMed] [Google Scholar]
- Summerfield G. P., Keenan J. P., Brodie N. J., Bellingham A. J. Bioluminescent assay of adenine nucleotides: rapid analysis of ATP and ADP in red cells and platelets using the LKB luminometer. Clin Lab Haematol. 1981;3(3):257–271. doi: 10.1111/j.1365-2257.1981.tb01340.x. [DOI] [PubMed] [Google Scholar]