Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1985 Jun;38(6):701–706. doi: 10.1136/jcp.38.6.701

Use of immunoblotting to identify antigenic differences between the yeast and mycelial phases of Candida albicans.

J P Burnie, R C Matthews, A Fox, S Tabaqchali
PMCID: PMC499271  PMID: 3891792

Abstract

Western blotting was applied to the analysis of Candida albicans in the yeast and mycelial phases in an attempt to recognise mycelial specific antigens which might be of serodiagnostic value. The antisera were prepared in rabbits by immunising them with pressates of C albicans type A NCTC 3153 in the yeast phase or the mycelial phase. These were blotted against C albicans in the yeast and mycelial phases and the yeast phase of C parapsilosis, C krusei, C tropicalis, and Torulopsis glabrata. Cross reactivity was greatest against C parapsilosis. One yeast specific mannoprotein was identified with a molecular weight of 49 000. No mycelial specific antigens could be identified.

Full text

PDF
701

Images in this article

703Image
on p.703
704Image
on p.704

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araj G. F., Hopfer R. L., Chesnut S., Fainstein V., Bodey G. P., Sr Diagnostic value of the enzyme-linked immunosorbent assay for detection of Candida albicans cytoplasmic antigen in sera of cancer patients. J Clin Microbiol. 1982 Jul;16(1):46–52. doi: 10.1128/jcm.16.1.46-52.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chaffin W. L., Stocco D. M. Cell wall proteins of Candida albicans. Can J Microbiol. 1983 Oct;29(10):1438–1444. doi: 10.1139/m83-220. [DOI] [PubMed] [Google Scholar]
  3. Ellsworth J. H., Reiss E., Bradley R. L., Chmel H., Armstrong D. Comparative serological and cutaneous reactivity of candidal cytoplasmic proteins and mannan separated by affinity for concanavalin A. J Clin Microbiol. 1977 Jan;5(1):91–99. doi: 10.1128/jcm.5.1.91-99.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HASENCLEVER H. F., MITCHELL W. O. Antigenic studies of Candida. I. Observation of two antigenic groups in Candida albicans. J Bacteriol. 1961 Oct;82:570–573. doi: 10.1128/jb.82.4.570-573.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kennedy J. F., Rosevear A. An assessment of the fractionation of carbohydrates on concanavalin A-sepharose 4B by affinity chromatography. J Chem Soc Perkin 1. 1973;19:2041–2046. doi: 10.1039/p19730002041. [DOI] [PubMed] [Google Scholar]
  6. Kozinn P. J., Taschdjian C. L., Goldberg P. K., Protzmann W. P., MacKenzie D. W., Remington J. S., Anderson S., Seelig M. S. Efficiency of serologic tests in the diagnosis of systemic candidiasis. Am J Clin Pathol. 1978 Dec;70(6):893–898. doi: 10.1093/ajcp/70.6.893. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lee K. L., Buckley H. R., Campbell C. C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans. Sabouraudia. 1975 Jul;13(2):148–153. doi: 10.1080/00362177585190271. [DOI] [PubMed] [Google Scholar]
  10. Lehmann P. F., Reiss E. Comparison by ELISA of serum anti-Candida albicans mannan IgG levels of a normal population and in diseased patients. Mycopathologia. 1980 Mar 17;70(2):89–93. doi: 10.1007/BF00443073. [DOI] [PubMed] [Google Scholar]
  11. Longbottom J. L., Brighton W. D., Edge G., Pepys J. Antibodies mediating type I skin test reactions to polysaccharide and protein antigens of Candida albicans. Clin Allergy. 1976 Jan;6(1):41–49. doi: 10.1111/j.1365-2222.1976.tb01410.x. [DOI] [PubMed] [Google Scholar]
  12. Manning M., Mitchell T. G. Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain, or temperature. J Bacteriol. 1980 Oct;144(1):258–273. doi: 10.1128/jb.144.1.258-273.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Munoz M., Estes G., Kilpatrick M., Di Salvo A., Virella G. Purification of cytoplasmic antigens from the mycelial phase of Candida albicans: possible advantages of its use in Candida serology. Mycopathologia. 1980 Aug 29;72(1):47–53. doi: 10.1007/BF00443050. [DOI] [PubMed] [Google Scholar]
  14. O'Connor C. G., Ashman L. K. Application of the nitrocellulose transfer technique and alkaline phosphatase conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures. J Immunol Methods. 1982 Oct 29;54(2):267–271. doi: 10.1016/0022-1759(82)90068-0. [DOI] [PubMed] [Google Scholar]
  15. Schwartz D. S., Larsh H. W. An effective medium for the selective growth of yeast or mycelial forms of Candida albicans: biochemical aspects of the two forms. Mycopathologia. 1980 Mar 17;70(2):67–75. doi: 10.1007/BF00443070. [DOI] [PubMed] [Google Scholar]
  16. Smail E. H., Jones J. M. Demonstration and solubilization of antigens expressed primarily on the surfaces of Candida albicans germ tubes. Infect Immun. 1984 Jul;45(1):74–81. doi: 10.1128/iai.45.1.74-81.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sundstrom P. M., Kenny G. E. Characterization of antigens specific to the surface of germ tubes of Candida albicans by immunofluorescence. Infect Immun. 1984 Mar;43(3):850–855. doi: 10.1128/iai.43.3.850-855.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Syverson R. E., Buckley H. R., Campbell C. C. Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans. Infect Immun. 1975 Nov;12(5):1184–1188. doi: 10.1128/iai.12.5.1184-1188.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES