There is an error in Table 1. The codons should be shown in bold. Please view the correct table here. The publisher apologizes for the error.
Table 1. Predicted masses and primer pairs for the PfoTPT transfection constructs.
The tyrosine (Y) codon in synthetic PfoTPT and the alanine (A) codon in Y10A are in bold. The predicted mass given for TMD10 is for the entire TMD1+TMD10 fusion protein. The HindIII cloning sites are underlined. A HindIII site is naturally found within TMD1 and one was added to the TMD10 forward primer.
| Construct | Predicted mass | Forward primer | Reverse primer |
|---|---|---|---|
| Synthetic PfoTPT | 39.0 kDa | 5’CACCATGAAAGATAACGAAAAAAAAAACGAATACGGTACGTT | 5’AAAGATAGAGTATAGAAATGCTCCAAAGATGGC |
| Y10A | 38.9 kDa | 5’CACCATGAAAGATAACGAAAAAAAAAACGAAGCTGGTACG | 5’AAAGATAGAGTATAGAAATGCTCCAAAGATGGC |
| TMD1to9 | 39.5 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TATAATTATTGATGAAACAATGATAACA |
| TMD1to8 | 36.4 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’AAGGCACATAAAAGCAACTTC |
| TMD1to6 | 27.0 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TGCATAAATAGATCTAATAGATGAACC |
| TMD1to5 | 24.0 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TTTCATAGATGCACAAACAACA |
| TMD1to2 | 14.2 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’ACTTATCCAATATATAAATATAAATATCCAT |
| TMD1 | 10.8 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TAAAGCTTTTTTATTATCTACATTATATAATAC |
| TMD10 | 10.55 kDa | 5’CACCAAGCTTTATTTAAAACACAAATAACGTTACTTGGA | 5’AAAGATTGAATACAAGAAAGCACCGAATATTG |
Reference
- 1.Lim L, Sayers CP, Goodman CD, McFadden GI (2016) Targeting of a Transporter to the Outer Apicoplast Membrane in the Human Malaria Parasite Plasmodium falciparum. PLoS ONE 11(7): e0159603 doi:10.1371/journal.pone.0159603 [DOI] [PMC free article] [PubMed] [Google Scholar]
