Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1992 Sep 15;89(18):8443–8447. doi: 10.1073/pnas.89.18.8443

Genomic structure and chromosomal mapping of the mouse N-cadherin gene.

S Miyatani 1, N G Copeland 1, D J Gilbert 1, N A Jenkins 1, M Takeichi 1
PMCID: PMC49936  PMID: 1528849

Abstract

N-cadherin is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). In this study, we determined the structure of the mouse N-cadherin gene by analyzing overlapping genomic clones obtained from a mouse genomic library. This gene consists of 16 exons that disperse over greater than 200 kilobases of genomic DNA. This large size of the N-cadherin gene, compared with its cDNA (4.3 kilobases), is ascribed to the fact that the first and second introns are 34.2 kilobases and greater than 100 kilobases long, respectively. When the N-cadherin gene was compared with that of L-CAM and P-cadherin, the exon-intron boundaries were found to be fully conserved between them, except that the P-cadherin first exon includes the first and second exons of the other two genes. Also, the second intron, which is equivalent to the first intron in P-cadherin, is exceptionally large and this structural feature is conserved in all of these genes. An interesting feature of the N-cadherin gene is that this gene has an extra 16th exon that is almost identical to the other exon, 100% in the coding region and 99% in the 3' untranslated region in the nucleotide level. We also determined the chromosomal localization of the N-cadherin gene by interspecific backcross analysis and found that this gene is localized in the proximal region of mouse chromosome 18. The E- and P-cadherin genes are tightly linked and located on chromosome 8 in this species. Thus, N-cadherin is unlinked to these other cadherin loci.

Full text

PDF
8443

Images in this article

8445Image
on p.8445
8446Image
on p.8446

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collins J. E., Legan P. K., Kenny T. P., MacGarvie J., Holton J. L., Garrod D. R. Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desmosomal adhesion molecules with heterogeneous cytoplasmic domains. J Cell Biol. 1991 Apr;113(2):381–391. doi: 10.1083/jcb.113.2.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Copeland N. G., Jenkins N. A. Development and applications of a molecular genetic linkage map of the mouse genome. Trends Genet. 1991 Apr;7(4):113–118. doi: 10.1016/0168-9525(91)90455-y. [DOI] [PubMed] [Google Scholar]
  3. Cox R. D., Copeland N. G., Jenkins N. A., Lehrach H. Interspersed repetitive element polymerase chain reaction product mapping using a mouse interspecific backcross. Genomics. 1991 Jun;10(2):375–384. doi: 10.1016/0888-7543(91)90322-6. [DOI] [PubMed] [Google Scholar]
  4. Eistetter H. R., Adolph S., Ringwald M., Simon-Chazottes D., Schuh R., Guénet J. L., Kemler R. Chromosomal mapping of the structural gene coding for the mouse cell adhesion molecule uvomorulin. Proc Natl Acad Sci U S A. 1988 May;85(10):3489–3493. doi: 10.1073/pnas.85.10.3489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gallin W. J., Sorkin B. C., Edelman G. M., Cunningham B. A. Sequence analysis of a cDNA clone encoding the liver cell adhesion molecule, L-CAM. Proc Natl Acad Sci U S A. 1987 May;84(9):2808–2812. doi: 10.1073/pnas.84.9.2808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hatta K., Nose A., Nagafuchi A., Takeichi M. Cloning and expression of cDNA encoding a neural calcium-dependent cell adhesion molecule: its identity in the cadherin gene family. J Cell Biol. 1988 Mar;106(3):873–881. doi: 10.1083/jcb.106.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hatta M., Miyatani S., Copeland N. G., Gilbert D. J., Jenkins N. A., Takeichi M. Genomic organization and chromosomal mapping of the mouse P-cadherin gene. Nucleic Acids Res. 1991 Aug 25;19(16):4437–4441. doi: 10.1093/nar/19.16.4437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holton J. L., Kenny T. P., Legan P. K., Collins J. E., Keen J. N., Sharma R., Garrod D. R. Desmosomal glycoproteins 2 and 3 (desmocollins) show N-terminal similarity to calcium-dependent cell-cell adhesion molecules. J Cell Sci. 1990 Oct;97(Pt 2):239–246. doi: 10.1242/jcs.97.2.239. [DOI] [PubMed] [Google Scholar]
  9. Jenkins N. A., Copeland N. G., Taylor B. A., Lee B. K. Organization, distribution, and stability of endogenous ecotropic murine leukemia virus DNA sequences in chromosomes of Mus musculus. J Virol. 1982 Jul;43(1):26–36. doi: 10.1128/jvi.43.1.26-36.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koch P. J., Walsh M. J., Schmelz M., Goldschmidt M. D., Zimbelmann R., Franke W. W. Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur J Cell Biol. 1990 Oct;53(1):1–12. [PubMed] [Google Scholar]
  11. Mahoney P. A., Weber U., Onofrechuk P., Biessmann H., Bryant P. J., Goodman C. S. The fat tumor suppressor gene in Drosophila encodes a novel member of the cadherin gene superfamily. Cell. 1991 Nov 29;67(5):853–868. doi: 10.1016/0092-8674(91)90359-7. [DOI] [PubMed] [Google Scholar]
  12. Mansouri A., Spurr N., Goodfellow P. N., Kemler R. Characterization and chromosomal localization of the gene encoding the human cell adhesion molecule uvomorulin. Differentiation. 1988 Jun;38(1):67–71. doi: 10.1111/j.1432-0436.1988.tb00593.x. [DOI] [PubMed] [Google Scholar]
  13. Miyatani S., Shimamura K., Hatta M., Nagafuchi A., Nose A., Matsunaga M., Hatta K., Takeichi M. Neural cadherin: role in selective cell-cell adhesion. Science. 1989 Aug 11;245(4918):631–635. doi: 10.1126/science.2762814. [DOI] [PubMed] [Google Scholar]
  14. Nagafuchi A., Shirayoshi Y., Okazaki K., Yasuda K., Takeichi M. Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. Nature. 1987 Sep 24;329(6137):341–343. doi: 10.1038/329341a0. [DOI] [PubMed] [Google Scholar]
  15. Nose A., Nagafuchi A., Takeichi M. Isolation of placental cadherin cDNA: identification of a novel gene family of cell-cell adhesion molecules. EMBO J. 1987 Dec 1;6(12):3655–3661. doi: 10.1002/j.1460-2075.1987.tb02698.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Parker A. E., Wheeler G. N., Arnemann J., Pidsley S. C., Ataliotis P., Thomas C. L., Rees D. A., Magee A. I., Buxton R. S. Desmosomal glycoproteins II and III. Cadherin-like junctional molecules generated by alternative splicing. J Biol Chem. 1991 Jun 5;266(16):10438–10445. [PubMed] [Google Scholar]
  17. Ringwald M., Schuh R., Vestweber D., Eistetter H., Lottspeich F., Engel J., Dölz R., Jähnig F., Epplen J., Mayer S. The structure of cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion. EMBO J. 1987 Dec 1;6(12):3647–3653. doi: 10.1002/j.1460-2075.1987.tb02697.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sorkin B. C., Gallin W. J., Edelman G. M., Cunningham B. A. Genes for two calcium-dependent cell adhesion molecules have similar structures and are arranged in tandem in the chicken genome. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11545–11549. doi: 10.1073/pnas.88.24.11545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sorkin B. C., Hemperly J. J., Edelman G. M., Cunningham B. A. Structure of the gene for the liver cell adhesion molecule, L-CAM. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7617–7621. doi: 10.1073/pnas.85.20.7617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Takeichi M. Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 1991 Mar 22;251(5000):1451–1455. doi: 10.1126/science.2006419. [DOI] [PubMed] [Google Scholar]
  21. Takeichi M. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis. Development. 1988 Apr;102(4):639–655. doi: 10.1242/dev.102.4.639. [DOI] [PubMed] [Google Scholar]
  22. Walsh F. S., Barton C. H., Putt W., Moore S. E., Kelsell D., Spurr N., Goodfellow P. N. N-cadherin gene maps to human chromosome 18 and is not linked to the E-cadherin gene. J Neurochem. 1990 Sep;55(3):805–812. doi: 10.1111/j.1471-4159.1990.tb04563.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES