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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1985 Aug;38(8):937–941. doi: 10.1136/jcp.38.8.937

Misuse and interlaboratory test reproducibility of API 20E system.

B Holmes, C A Dawson
PMCID: PMC499401  PMID: 3897293

Abstract

One hundred strains were referred to us for identification because they apparently could not be identified satisfactorily with the API 20E system (appareils et procédés d'identification). The inability to identify 31 strains was due primarily to failure to follow the manufacturer's instructions. Twenty six further strains were found to have been correctly identified by the sender's own API 20E results, so that only the remaining 43 strains definitely fell into the category for which our identification service was intended. Eighteen of the 43 strains not identified by the sender were identified by us using the API 20E system, and several possible reasons are given to explain the differences in these results. The remaining 25 strains either could not be identified by us on the API 20E system or, in the case of 13, they could not be identified by our conventional system and therefore no comparison could be made. The average interlaboratory probability of errors for the API 20E tests was 6.1%.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butler D. A., Lobregat C. M., Gavan T. L. Reproducibility of the analytab (API 20E) system. J Clin Microbiol. 1975 Oct;2(4):322–326. doi: 10.1128/jcm.2.4.322-326.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. D'Amato R. F., Holmes B., Bottone E. J. The systems approach to diagnostic microbiology. Crit Rev Microbiol. 1981;9(1):1–44. doi: 10.3109/10408418109104485. [DOI] [PubMed] [Google Scholar]
  3. Holmes B., Willcox W. R., Lapage S. P. Identification of Enterobacteriaceae by the API 20E system. J Clin Pathol. 1978 Jan;31(1):22–30. doi: 10.1136/jcp.31.1.22. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holmes B., Willcox W. R., Lapage S. P., Malnick H. Test reproducibility of the API (20E), Enterotube, and Pathotec systems. J Clin Pathol. 1977 Apr;30(4):381–387. doi: 10.1136/jcp.30.4.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lapage S. P., Bascomb S., Willcox W. R., Curtis M. A. Identification of bacteria by computer: general aspects and perspectives. J Gen Microbiol. 1973 Aug;77(2):273–290. doi: 10.1099/00221287-77-2-273. [DOI] [PubMed] [Google Scholar]
  6. Murray P. R. Standardization of the Analytab Enteric (API 20E) system to increase accuracy and reproducibility of the test for biotype characterization of bacteria. J Clin Microbiol. 1978 Jul;8(1):46–49. doi: 10.1128/jcm.8.1.46-49.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Snell J. J., Lapage S. P. Carbon source utilization tests as an aid to the classification of non-fermenting gram-negative bacteria. J Gen Microbiol. 1973 Jan;74(1):9–20. doi: 10.1099/00221287-74-1-9. [DOI] [PubMed] [Google Scholar]
  8. Willcox W. R., Lapage S. P., Holmes B. A review of numerical methods in bacterial identification. Antonie Van Leeuwenhoek. 1980;46(3):233–299. doi: 10.1007/BF00453024. [DOI] [PubMed] [Google Scholar]

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