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Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1985 Oct;38(10):1085–1092. doi: 10.1136/jcp.38.10.1085

Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.

J Burns, V T Chan, J A Jonasson, K A Fleming, S Taylor, J O McGee
PMCID: PMC499446  PMID: 3902895

Abstract

A fast and sensitive method for detecting biotinylated deoxyribonucleic acid (DNA) probes was used for sex determination of cells and tissues by in situ hybridisation of a probe "specific" for the Y chromosome (pHY 2.1). Within 24 hours this procedure visualizes the Y chromosome in fetal and adult cells and tissue, without background noise. This procedure should facilitate antenatal determination of sex on small numbers of uncultured cells. The sensitivity of the procedure also permits the chromosomal assignment of genes present in low copy number.

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Selected References

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