Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1985 Oct;38(10):1132–1138. doi: 10.1136/jcp.38.10.1132

Four hour identification of Enterobacteriaceae with the API Rapid 20E and Micro-ID systems.

B Holmes, P S Humphry
PMCID: PMC499454  PMID: 3902898

Abstract

One hundred strains of Enterobacteriaceae were examined in parallel with the API Rapid 20E and Micro-ID commercial four hour identification systems. With the API Rapid 20E system 78% of the strains were correctly identified, 15% were not identified, and 7% were misidentified. The respective figures with the Micro-ID system were 74%, 11%, and 15%.

Full text

PDF
1132

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Gardner B. B., Clark S. J., Matsen J. M. Comparison of micro-ID, API 20E, and conventional media systems in identification of Enterobacteriaceae. J Clin Microbiol. 1978 Jun;7(6):507–513. doi: 10.1128/jcm.7.6.507-513.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Altwegg M. Performance of two four-hour identification systems with atypical strains of Enterobacteriaceae. Eur J Clin Microbiol. 1983 Dec;2(6):529–533. doi: 10.1007/BF02016560. [DOI] [PubMed] [Google Scholar]
  3. Appelbaum P. C., Olmstead C. C. Evaluation of Gram-stain screen and Micro-ID methods for direct identification of Enterobacteriaceae from urines. Med Microbiol Immunol. 1982;170(3):173–184. doi: 10.1007/BF02298197. [DOI] [PubMed] [Google Scholar]
  4. Appelbaum P. C., Schick S. F., Kellogg J. A. Evaluation of the four-hour Micro-ID technique for direct identification of oxidase-negative, Gram-negative rods from blood cultures. J Clin Microbiol. 1980 Oct;12(4):533–537. doi: 10.1128/jcm.12.4.533-537.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bale M. J., Matsen J. M. Time-motion and cost comparison study of micro-ID, API 20E, and conventional biochemical testing in identification of Enterobacteriaceae. J Clin Microbiol. 1981 Dec;14(6):665–670. doi: 10.1128/jcm.14.6.665-670.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Barry A. L., Badal R. E. Rapid identification of Enterobacteriaceae with the micro-ID system versus API 20E and conventional media. J Clin Microbiol. 1979 Sep;10(3):293–298. doi: 10.1128/jcm.10.3.293-298.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bascomb S., Lapage S. P., Curtis M. A., Willcox W. R. Identification of bacteria by computer: identification of reference strains. J Gen Microbiol. 1973 Aug;77(2):291–315. doi: 10.1099/00221287-77-2-291. [DOI] [PubMed] [Google Scholar]
  8. Blazevic D. J., Mackay D. L., Warwood N. M. Comparison of Micro-ID and API 20E systems for identification of Enterobacteriaceae. J Clin Microbiol. 1979 May;9(5):605–608. doi: 10.1128/jcm.9.5.605-608.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Buesching W. J., Rhoden D. L., Esaias A. O., Smith P. B., Washington J. A., 2nd Evaluation of the modified Micro-ID system for identification of Enterobacteriaceae. J Clin Microbiol. 1979 Oct;10(4):454–458. doi: 10.1128/jcm.10.4.454-458.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Burdash N. M., West M. E. Effect of refrigeration on inoculated Micro-ID strips. J Clin Microbiol. 1981 Sep;14(3):350–351. doi: 10.1128/jcm.14.3.350-351.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. D'Amato R. F., Holmes B., Bottone E. J. The systems approach to diagnostic microbiology. Crit Rev Microbiol. 1981;9(1):1–44. doi: 10.3109/10408418109104485. [DOI] [PubMed] [Google Scholar]
  12. Edberg S. C., Atkinson B., Chambers C., Moore M. H., Palumbo L., Zorzon C. F., Singer J. M. Clinical evaluation of the MICRO-ID, API 20E, and conventional media systems for identification of Enterobacteriacea. J Clin Microbiol. 1979 Aug;10(2):161–167. doi: 10.1128/jcm.10.2.161-167.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Edberg S. C., Clare D., Moore M. H., Singer J. M. Rapid identification of Enterobacteriaceae from blood cultures with the Micro-ID system. J Clin Microbiol. 1979 Nov;10(5):693–697. doi: 10.1128/jcm.10.5.693-697.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Edberg S. C., Melton E., Singer J. M. Rapid biochemical characterization of Haemophilus species by using the micro-ID. J Clin Microbiol. 1980 Jan;11(1):22–26. doi: 10.1128/jcm.11.1.22-26.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gooch W. M., 3rd, Hill G. A. Comparison of micro-ID and API 20E in rapid identification of Enterobacteriaceae. J Clin Microbiol. 1982 May;15(5):885–890. doi: 10.1128/jcm.15.5.885-890.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Izard D., Gavini F., Ochin D., Enayeh E., Leclerc H. Evaluation of Micro-ID, for identification of Enterobacteriaceae. Pathol Biol (Paris) 1980 Dec;28(10):681–684. [PubMed] [Google Scholar]
  17. Kelly M. T., Hale D. C., Matsen J. M. Rapid identification by the Micro-ID system of Enterobacteriaceae detected by urine screening. J Clin Microbiol. 1981 Sep;14(3):295–297. doi: 10.1128/jcm.14.3.295-297.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kelly M. T., Latimer J. M. Comparison of the automicrobic system with API, enterotube, micro-ID, micro-media systems, and conventional methods for identification of Enterobacteriaceae. J Clin Microbiol. 1980 Nov;12(5):659–662. doi: 10.1128/jcm.12.5.659-662.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Keville M. W., Doern G. V. Evaluation of the DMS Rapid E system for identification of clinical isolates of the family Enterobacteriaceae. J Clin Microbiol. 1984 Nov;20(5):1010–1011. doi: 10.1128/jcm.20.5.1010-1011.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lapage S. P., Bascomb S., Willcox W. R., Curtis M. A. Identification of bacteria by computer: general aspects and perspectives. J Gen Microbiol. 1973 Aug;77(2):273–290. doi: 10.1099/00221287-77-2-273. [DOI] [PubMed] [Google Scholar]
  21. Malloy P. J., Ducate M. J., Schreckenberger P. C. Comparison of four rapid methods for identification of Enterobacteriaceae from blood cultures. J Clin Microbiol. 1983 Mar;17(3):493–499. doi: 10.1128/jcm.17.3.493-499.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mounier M., Denis F. Four-hour direct identification of Enterobacteriaceae in blood cultures. Eur J Clin Microbiol. 1983 Dec;2(6):593–595. doi: 10.1007/BF02016573. [DOI] [PubMed] [Google Scholar]
  23. Murray P. R., Gauthier A., Niles A. Evaluation of the Quantum II and Rapid E identification systems. J Clin Microbiol. 1984 Sep;20(3):509–514. doi: 10.1128/jcm.20.3.509-514.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wellstood S. Comparison of Micro-ID, a modified Minitek system, and conventional methods for four-hour identification of Enterobacteriacae directly from blood cultures. Am J Med Technol. 1982 Sep;48(9):769–773. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES