Table 2.

Steps and time for the 8 min automated mini-SPE method

Step	Description	Time (s)
1	Wash the 1 mL syringe with MeCN (2 pumps of 0.5 mL each)	30
2	Load 300 µL extract from AS vial in Tray1 into 1 mL syringe	10
3	Place mini-cartridge above collection AS vial (with glass insert) in Tray2	10
4	Elute extract through mini-cartridge at 2 µL s−1	150
5	Discard mini-cartridge into waste receptacle	5
6	Wash the 1 mL syringe with 1/1/1 MeCN/MeOH/water (2 pumps of 0.5 mL each)	30
7	Wash the 1 mL syringe with MeCN (4 pumps of 0.5 mL each)	45
8	Switch to 100 µL syringe and wash with MeCN (2 pumps of 50 µL each)	50
9	Add 25 µL MeCN to collection AS vial (with glass insert) in Tray2	10
10	Add 25 µL AP + QC sol’n to collection AS vial (with glass insert) in Tray2	10
11	Wash the 100 µL syringe with 1/1/1 MeCN/MeOH/water (5 pumps of 50 µL each)	50
12	Wash the 100 µL syringe with MeCN (3 pumps of 50 µL each)	40
13	Switch to 1 mL syringe and move to home position	40

The cleanup itself only took ≈3 min, and syringe wash and exchange steps took ≈5 min