Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1986 Jan;39(1):51–57. doi: 10.1136/jcp.39.1.51

Study of childhood renal tumours using antisera to fibronectin, laminin, and epithelial membrane antigen.

S Kumar, T Carr, H B Marsden, M C Calabuig-Crespo
PMCID: PMC499612  PMID: 3005371

Abstract

Using a peroxidase-antiperoxidase staining procedure, formalin fixed paraffin embedded sections of fetal and normal kidney; benign (mesoblastic nephroma); and malignant tumours (Wilms' tumour, clear cell renal carcinoma, rhabdoid renal tumour, and bone metastasising renal tumour of childhood (BMRTC] were examined for their reactivity with antisera to fibronectin, laminin, and epithelial membrane antigen. Mesoblastic nephroma contained fibronectin but no laminin. Most Wilms' tumours lacked both fibronectin and laminin; 50% of rhabdoid renal tumours were positive for fibronectin and laminin--rhabdoid tumours as recognised morphologically may, in fact, be two separate entities. BMRTC and clear cell renal carcinoma lacked both fibronectin and laminin. Epithelial membrane antigen was present in most of the tubular Wilms' tumour but absent in blastemal Wilms' tumours. The presence of epithelial membrane antigen in rhabdoid tumours was surprising, as histologically, this type of tumour shows no sign of epithelial differentiation. Epithelial membrane antigen antiserum stained clear cell renal carcinomas: epithelial membrane antigen is found in the distal and not the proximal tubules of fetal and normal kidneys. Thus an obvious interpretation is that clear cell renal carcinomas originate from distal rather than from proximal tubules, as has always been thought. On the basis of these results and data from other published findings some possible histogenetic origins of childhood renal tumours were proposed.

Full text

PDF
51

Images in this article

54Image
on p.54
54Image
on p.54
54Image
on p.54
54Image
on p.54
54Image
on p.54
54Image
on p.54
54Image
on p.54
54Image
on p.54

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckwith J. B. Wilms' tumor and other renal tumors of childhood: a selective review from the National Wilms' Tumor Study Pathology Center. Hum Pathol. 1983 Jun;14(6):481–492. doi: 10.1016/s0046-8177(83)80003-3. [DOI] [PubMed] [Google Scholar]
  2. Fisher J. H., Miller Y. E., Sparkes R. S., Bateman J. B., Kimmel K. A., Carey T. E., Rodell T., Shoemaker S. A., Scoggin C. H. Wilms' tumor-aniridia association: segregation of affected chromosome in somatic cell hybrids, identification of cell surface antigen associated with deleted area, and regional mapping of c-Ha-ras-1 oncogene, insulin gene, and beta-globin gene. Somat Cell Mol Genet. 1984 Sep;10(5):455–464. doi: 10.1007/BF01534850. [DOI] [PubMed] [Google Scholar]
  3. Haas J. E., Bonadio J. F., Beckwith J. B. Clear cell sarcoma of the kidney with emphasis on ultrastructural studies. Cancer. 1984 Dec 15;54(12):2978–2987. doi: 10.1002/1097-0142(19841215)54:12<2978::aid-cncr2820541228>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  4. Haas J. E., Palmer N. F., Weinberg A. G., Beckwith J. B. Ultrastructure of malignant rhabdoid tumor of the kidney. A distinctive renal tumor of children. Hum Pathol. 1981 Jul;12(7):646–657. doi: 10.1016/s0046-8177(81)80050-0. [DOI] [PubMed] [Google Scholar]
  5. Kumar S., Marsden H. B., Carr T., Kodet R. Mesoblastic nephroma contains fibronectin but lacks laminin. J Clin Pathol. 1985 May;38(5):507–511. doi: 10.1136/jcp.38.5.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lee Y. W., Gisser S. D. Squamous cell carcinoma of the tongue in a nine year renal transplant survivor: a case report with a discussion of the risk of development of epithelial carcinomas in renal transplant survivors. Cancer. 1978 Jan;41(1):1–6. doi: 10.1002/1097-0142(197801)41:1<1::aid-cncr2820410101>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  7. Marsden H. B., Lawler W., Carr T., Kumar S. A scoring system for Wilms' tumour: pathological study of the second Medical Research Council (MRC) trial. Int J Cancer. 1984 Mar 15;33(3):365–368. doi: 10.1002/ijc.2910330314. [DOI] [PubMed] [Google Scholar]
  8. Marsden H. B., Lawler W., Kumar P. M. Bone metastasizing renal tumor of childhood: morphological and clinical features, and differences from Wilms' tumor. Cancer. 1978 Oct;42(4):1922–1928. doi: 10.1002/1097-0142(197810)42:4<1922::aid-cncr2820420434>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  9. Sariola H., Ekblom P., Rapola J., Vaheri A., Timpl R. Extracellular matrix and epithelial differentiation of Wilms' tumor. Am J Pathol. 1985 Jan;118(1):96–107. [PMC free article] [PubMed] [Google Scholar]
  10. Sloane J. P., Ormerod M. G. Distribution of epithelial membrane antigen in normal and neoplastic tissues and it value in diagnostic tumor pathology. Cancer. 1981 Apr 1;47(7):1786–1795. doi: 10.1002/1097-0142(19810401)47:7<1786::aid-cncr2820470711>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  11. Snyder H. M., 3rd, Lack E. E., Chetty-Baktavizian A., Bauer S. B., Colodny A. H., Retik A. B. Congenital mesoblastic nephroma: relationship to other renal tumors of infancy. J Urol. 1981 Oct;126(4):513–516. doi: 10.1016/s0022-5347(17)54601-7. [DOI] [PubMed] [Google Scholar]
  12. Vogel A. M., Gown A. M., Caughlan J., Haas J. E., Beckwith J. B. Rhabdoid tumors of the kidney contain mesenchymal specific and epithelial specific intermediate filament proteins. Lab Invest. 1984 Feb;50(2):232–238. [PubMed] [Google Scholar]
  13. Wallace A. C., Nairn R. C. Renal tubular antigens in kidney tumors. Cancer. 1972 Apr;29(4):977–981. doi: 10.1002/1097-0142(197204)29:4<977::aid-cncr2820290444>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES