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. 1996 Dec;49(12):1005–1008. doi: 10.1136/jcp.49.12.1005

Association between Helicobacter pylori infection and serum pepsinogen concentrations in gastroduodenal disease.

K Matsumoto 1, N Konishi 1, M Ohshima 1, Y Hiasa 1, E Kimura 1, T Samori 1
PMCID: PMC499650  PMID: 9038739

Abstract

AIM: To investigate the association between Helicobacter pylori infection and serum pepsinogen (PG) 1 and 2 concentrations in various gastroduodenal diseases. METHODS: Serum PG1 and 2 concentrations and antibodies to H pylori were measured by enzyme linked immunosorbent assay (ELISA); gastric mucosal pH was assessed and urease activity in biopsy tissue was determined. A comparison of the ELISA and urease test results permitted division of the cases into positive, false positive, false negative and negative categories for control, gastritis, and ulcer groups. RESULTS: The gastric mucosal pH and serum PG2 in cases positive for H pylori were significantly increased in ulcer and gastritis cases compared with H pylori negative cases. Similar tendencies were observed for the false positive and false negative categories. CONCLUSIONS: A positive ELISA reaction for antibodies and an increased serum PG2 concentration are reliable indicators of H pylori infection.

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Selected References

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  1. Asaka M., Kimura T., Kudo M., Takeda H., Mitani S., Miyazaki T., Miki K., Graham D. Y. Relationship of Helicobacter pylori to serum pepsinogens in an asymptomatic Japanese population. Gastroenterology. 1992 Mar;102(3):760–766. doi: 10.1016/0016-5085(92)90156-s. [DOI] [PubMed] [Google Scholar]
  2. Biasco G., Paganelli G. M., Vaira D., Holton J., Di Febo G., Brillanti S., Miglioli M., Barbara L., Samloff I. M. Serum pepsinogen I and II concentrations and IgG antibody to Helicobacter pylori in dyspeptic patients. J Clin Pathol. 1993 Sep;46(9):826–828. doi: 10.1136/jcp.46.9.826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blaser M. J. Gastric Campylobacter-like organisms, gastritis, and peptic ulcer disease. Gastroenterology. 1987 Aug;93(2):371–383. doi: 10.1016/0016-5085(87)91028-6. [DOI] [PubMed] [Google Scholar]
  4. Blaser M. J. Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology. 1992 Feb;102(2):720–727. doi: 10.1016/0016-5085(92)90126-j. [DOI] [PubMed] [Google Scholar]
  5. Evans D. J., Jr, Evans D. G., Graham D. Y., Klein P. D. A sensitive and specific serologic test for detection of Campylobacter pylori infection. Gastroenterology. 1989 Apr;96(4):1004–1008. doi: 10.1016/0016-5085(89)91616-8. [DOI] [PubMed] [Google Scholar]
  6. Goodwin C. S., Blincow E., Peterson G., Sanderson C., Cheng W., Marshall B., Warren J. R., McCulloch R. Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa. J Infect Dis. 1987 Mar;155(3):488–494. doi: 10.1093/infdis/155.3.488. [DOI] [PubMed] [Google Scholar]
  7. Graham D. Y., Klein P. D., Evans D. J., Jr, Evans D. G., Alpert L. C., Opekun A. R., Boutton T. W. Campylobacter pylori detected noninvasively by the 13C-urea breath test. Lancet. 1987 May 23;1(8543):1174–1177. doi: 10.1016/s0140-6736(87)92145-3. [DOI] [PubMed] [Google Scholar]
  8. Graham D. Y., Opekun A., Lew G. M., Evans D. J., Jr, Klein P. D., Evans D. G. Ablation of exaggerated meal-stimulated gastrin release in duodenal ulcer patients after clearance of Helicobacter (Campylobacter) pylori infection. Am J Gastroenterol. 1990 Apr;85(4):394–398. [PubMed] [Google Scholar]
  9. Hunter F. M., Correa P., Fontham E., Ruiz B., Sobhan M., Samloff I. M. Serum pepsinogens as markers of response to therapy for Helicobacter pylori gastritis. Dig Dis Sci. 1993 Nov;38(11):2081–2086. doi: 10.1007/BF01297088. [DOI] [PubMed] [Google Scholar]
  10. Jones D. M., Eldridge J., Fox A. J., Sethi P., Whorwell P. J. Antibody to the gastric campylobacter-like organism ("Campylobacter pyloridis")--clinical correlations and distribution in the normal population. J Med Microbiol. 1986 Aug;22(1):57–62. doi: 10.1099/00222615-22-1-57. [DOI] [PubMed] [Google Scholar]
  11. Konishi N., Matsumoto K., Hiasa Y., Kitahori Y., Hayashi I., Matsuda H. Tissue and serum pepsinogen I and II in gastric cancer identified using immunohistochemistry and rapid ELISA. J Clin Pathol. 1995 Apr;48(4):364–367. doi: 10.1136/jcp.48.4.364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levine J. H., Michael J. R., Guarnieri T. Multifocal atrial tachycardia: a toxic effect of theophylline. Lancet. 1985 Jan 5;1(8419):12–14. doi: 10.1016/s0140-6736(85)90964-x. [DOI] [PubMed] [Google Scholar]
  13. Marshall B. J. Campylobacter pyloridis and gastritis. J Infect Dis. 1986 Apr;153(4):650–657. doi: 10.1093/infdis/153.4.650. [DOI] [PubMed] [Google Scholar]
  14. McColl K. E., Fullarton G. M., el Nujumi A. M., Macdonald A. M., Brown I. L., Hilditch T. E. Lowered gastrin and gastric acidity after eradication of Campylobacter pylori in duodenal ulcer. Lancet. 1989 Aug 26;2(8661):499–500. doi: 10.1016/s0140-6736(89)92105-3. [DOI] [PubMed] [Google Scholar]
  15. Miki K., Ichinose M., Shimizu A., Huang S. C., Oka H., Furihata C., Matsushima T., Takahashi K. Serum pepsinogens as a screening test of extensive chronic gastritis. Gastroenterol Jpn. 1987 Apr;22(2):133–141. doi: 10.1007/BF02774209. [DOI] [PubMed] [Google Scholar]
  16. Moss S. F., Calam J. Acid secretion and sensitivity to gastrin in patients with duodenal ulcer: effect of eradication of Helicobacter pylori. Gut. 1993 Jul;34(7):888–892. doi: 10.1136/gut.34.7.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Multicentre randomised clinical trial of chorion villus sampling and amniocentesis. First report. Canadian Collaborative CVS-Amniocentesis Clinical Trial Group. Lancet. 1989 Jan 7;1(8628):1–6. [PubMed] [Google Scholar]
  18. Oderda G., Vaira D., Holton J., Dowsett J. F., Ansaldi N. Serum pepsinogen I and IgG antibody to Campylobacter pylori in non-specific abdominal pain in childhood. Gut. 1989 Jul;30(7):912–916. doi: 10.1136/gut.30.7.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Perez-Perez G. I., Dworkin B. M., Chodos J. E., Blaser M. J. Campylobacter pylori antibodies in humans. Ann Intern Med. 1988 Jul 1;109(1):11–17. doi: 10.7326/0003-4819-109-1-11. [DOI] [PubMed] [Google Scholar]
  20. Rathbone B. J., Wyatt J. I., Worsley B. W., Shires S. E., Trejdosiewicz L. K., Heatley R. V., Losowsky M. S. Systemic and local antibody responses to gastric Campylobacter pyloridis in non-ulcer dyspepsia. Gut. 1986 Jun;27(6):642–647. doi: 10.1136/gut.27.6.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rauws E. A., Langenberg W., Houthoff H. J., Zanen H. C., Tytgat G. N. Campylobacter pyloridis-associated chronic active antral gastritis. A prospective study of its prevalence and the effects of antibacterial and antiulcer treatment. Gastroenterology. 1988 Jan;94(1):33–40. [PubMed] [Google Scholar]
  22. Samloff I. M. Pepsinogens I and II: purification from gastric mucosa and radioimmunoassay in serum. Gastroenterology. 1982 Jan;82(1):26–33. [PubMed] [Google Scholar]
  23. Samloff I. M. Slow moving protease and the seven pepsinogens. Electrophoretic demontration of the existence of eight proteolytic fractions in human gastric mucosa. Gastroenterology. 1969 Dec;57(6):659–669. [PubMed] [Google Scholar]
  24. Samloff I. M., Varis K., Ihamaki T., Siurala M., Rotter J. I. Relationships among serum pepsinogen I, serum pepsinogen II, and gastric mucosal histology. A study in relatives of patients with pernicious anemia. Gastroenterology. 1982 Jul;83(1 Pt 2):204–209. [PubMed] [Google Scholar]
  25. Sipponen P., Samloff I. M., Saukkonen M., Varis K. Serum pepsinogens I and II and gastric mucosal histology after partial gastrectomy. Gut. 1985 Nov;26(11):1179–1182. doi: 10.1136/gut.26.11.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sugiyama T., Imai K., Yoshida H., Takayama Y., Yabana T., Yokota K., Oguma K., Yachi A. A novel enzyme immunoassay for serodiagnosis of Helicobacter pylori infection. Gastroenterology. 1991 Jul;101(1):77–83. doi: 10.1016/0016-5085(91)90462-t. [DOI] [PubMed] [Google Scholar]
  27. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. 1983 Jun 4;1(8336):1273–1275. [PubMed] [Google Scholar]
  28. Vaira D., Holton J., Cairns S. R., Falzon M., Polydorou A., Dowsett J. F., Salmon P. R. Antibody titres to Campylobacter pylori after treatment for gastritis. BMJ. 1988 Aug 6;297(6645):397–397. doi: 10.1136/bmj.297.6645.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wagner S., Haruma K., Gladziwa U., Soudah B., Gebel M., Bleck J., Schmidt H., Manns M. Helicobacter pylori infection and serum pepsinogen A, pepsinogen C, and gastrin in gastritis and peptic ulcer: significance of inflammation and effect of bacterial eradication. Am J Gastroenterol. 1994 Aug;89(8):1211–1218. [PubMed] [Google Scholar]
  30. Westerveld B. D., Pals G., Lamers C. B., Defize J., Pronk J. C., Frants R. R., Ooms E. C., Kreuning J., Kostense P. J., Eriksson A. W. Clinical significance of pepsinogen A isozymogens, serum pepsinogen A and C levels, and serum gastrin levels. Cancer. 1987 Mar 1;59(5):952–958. doi: 10.1002/1097-0142(19870301)59:5<952::aid-cncr2820590517>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  31. Yoshitake S., Imagawa M., Ishikawa E., Niitsu Y., Urushizaki I., Nishiura M., Kanazawa R., Kurosaki H., Tachibana S., Nakazawa N. Mild and efficient conjugation of rabbit Fab' and horseradish peroxidase using a maleimide compound and its use for enzyme immunoassay. J Biochem. 1982 Nov;92(5):1413–1424. doi: 10.1093/oxfordjournals.jbchem.a134065. [DOI] [PubMed] [Google Scholar]
  32. von Wulffen H., Heesemann J., Bützow G. H., Löning T., Laufs R. Detection of Campylobacter pyloridis in patients with antrum gastritis and peptic ulcers by culture, complement fixation test, and immunoblot. J Clin Microbiol. 1986 Nov;24(5):716–720. doi: 10.1128/jcm.24.5.716-720.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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