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. 1997 Jan;50(1):59–63. doi: 10.1136/jcp.50.1.59

Widespread presence of cytomegalovirus DNA in tissues of healthy trauma victims.

R M Hendrix 1, M Wagenaar 1, R L Slobbe 1, C A Bruggeman 1
PMCID: PMC499715  PMID: 9059359

Abstract

AIMS: To determine the localisation of human cytomegalovirus (CMV) DNA in abdominal aorta, spleen, and transplantable organs, such as kidney, pancreas, and liver, obtained from healthy individuals; to characterise the cell type(s) in these tissues that serve as a reservoir for latent CMV. METHODS: CMV DNA was detected by dot blot DNA hybridisation and in situ DNA hybridisation with a probe for CMV major immediate early sequences (UL123) and nested PCR with primers derived from the CMV major immediate early (IE) gene exon 4 (UL123ex4). Samples of liver, abdominal aorta, spleen, kidney, and pancreas were obtained at necropsy or from donor kidneys from healthy subjects. RESULTS: CMV DNA was detected in most tissue samples using dot blot hybridisation and nested PCR. In situ hybridisation demonstrated that, in addition to smooth muscle cells in the arterial wall, hepatocytes, tubular and glomerular kidney cells, splenic red pulp cells, and pancreatic acinar cells also harboured CMV DNA. CMV DNA was detected in seropositive and in some seronegative subjects. CONCLUSION: CMV DNA is widely distributed in organs of healthy subjects. CMV DNA was found in various cell types in several organs, suggesting that during latency, CMV DNA is present thoughout the body.

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Selected References

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  1. Balthesen M., Messerle M., Reddehase M. J. Lungs are a major organ site of cytomegalovirus latency and recurrence. J Virol. 1993 Sep;67(9):5360–5366. doi: 10.1128/jvi.67.9.5360-5366.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bevan I. S., Daw R. A., Day P. J., Ala F. A., Walker M. R. Polymerase chain reaction for detection of human cytomegalovirus infection in a blood donor population. Br J Haematol. 1991 May;78(1):94–99. doi: 10.1111/j.1365-2141.1991.tb04388.x. [DOI] [PubMed] [Google Scholar]
  3. Chou S. W. Acquisition of donor strains of cytomegalovirus by renal-transplant recipients. N Engl J Med. 1986 May 29;314(22):1418–1423. doi: 10.1056/NEJM198605293142205. [DOI] [PubMed] [Google Scholar]
  4. Collins T., Pomeroy C., Jordan M. C. Detection of latent cytomegalovirus DNA in diverse organs of mice. J Infect Dis. 1993 Sep;168(3):725–729. doi: 10.1093/infdis/168.3.725. [DOI] [PubMed] [Google Scholar]
  5. Hendrix M. G., Daemen M., Bruggeman C. A. Cytomegalovirus nucleic acid distribution within the human vascular tree. Am J Pathol. 1991 Mar;138(3):563–567. [PMC free article] [PubMed] [Google Scholar]
  6. Hendrix M. G., Dormans P. H., Kitslaar P., Bosman F., Bruggeman C. A. The presence of cytomegalovirus nucleic acids in arterial walls of atherosclerotic and nonatherosclerotic patients. Am J Pathol. 1989 May;134(5):1151–1157. [PMC free article] [PubMed] [Google Scholar]
  7. Hendrix M. G., Salimans M. M., van Boven C. P., Bruggeman C. A. High prevalence of latently present cytomegalovirus in arterial walls of patients suffering from grade III atherosclerosis. Am J Pathol. 1990 Jan;136(1):23–28. [PMC free article] [PubMed] [Google Scholar]
  8. Ho M. Epidemiology of cytomegalovirus infections. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S701–S710. doi: 10.1093/clinids/12.supplement_7.s701. [DOI] [PubMed] [Google Scholar]
  9. Klotman M. E., Henry S. C., Greene R. C., Brazy P. C., Klotman P. E., Hamilton J. D. Detection of mouse cytomegalovirus nucleic acid in latently infected mice by in vitro enzymatic amplification. J Infect Dis. 1990 Feb;161(2):220–225. doi: 10.1093/infdis/161.2.220. [DOI] [PubMed] [Google Scholar]
  10. Kraat Y. J., Hendrix M. G., Wijnen R. M., Peltenburg H. G., van Hooff J. P., Geelen J. L., Bruggeman C. A. Detection of latent human cytomegalovirus in organ tissue and the correlation with serological status. Transpl Int. 1992;5 (Suppl 1):S613–S616. doi: 10.1007/978-3-642-77423-2_180. [DOI] [PubMed] [Google Scholar]
  11. Kraat Y. J., Hendrix R. M., Landini M. P., Bruggeman C. A. Comparison of four techniques for detection of antibodies to cytomegalovirus. J Clin Microbiol. 1992 Feb;30(2):522–524. doi: 10.1128/jcm.30.2.522-524.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Melnick J. L., Petrie B. L., Dreesman G. R., Burek J., McCollum C. H., DeBakey M. E. Cytomegalovirus antigen within human arterial smooth muscle cells. Lancet. 1983 Sep 17;2(8351):644–647. doi: 10.1016/s0140-6736(83)92529-1. [DOI] [PubMed] [Google Scholar]
  13. Myerson D., Hackman R. C., Nelson J. A., Ward D. C., McDougall J. K. Widespread presence of histologically occult cytomegalovirus. Hum Pathol. 1984 May;15(5):430–439. doi: 10.1016/s0046-8177(84)80076-3. [DOI] [PubMed] [Google Scholar]
  14. Persing D. H. Polymerase chain reaction: trenches to benches. J Clin Microbiol. 1991 Jul;29(7):1281–1285. doi: 10.1128/jcm.29.7.1281-1285.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  16. Schönian U., Crombach M., Maisch B. Assessment of cytomegalovirus DNA and protein expression in patients with myocarditis. Clin Immunol Immunopathol. 1993 Aug;68(2):229–233. doi: 10.1006/clin.1993.1123. [DOI] [PubMed] [Google Scholar]
  17. Stanier P., Taylor D. L., Kitchen A. D., Wales N., Tryhorn Y., Tyms A. S. Persistence of cytomegalovirus in mononuclear cells in peripheral blood from blood donors. BMJ. 1989 Oct 7;299(6704):897–898. doi: 10.1136/bmj.299.6704.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taylor-Wiedeman J., Hayhurst G. P., Sissons J. G., Sinclair J. H. Polymorphonuclear cells are not sites of persistence of human cytomegalovirus in healthy individuals. J Gen Virol. 1993 Feb;74(Pt 2):265–268. doi: 10.1099/0022-1317-74-2-265. [DOI] [PubMed] [Google Scholar]
  19. Toorkey C. B., Carrigan D. R. Immunohistochemical detection of an immediate early antigen of human cytomegalovirus in normal tissues. J Infect Dis. 1989 Nov;160(5):741–751. doi: 10.1093/infdis/160.5.741. [DOI] [PubMed] [Google Scholar]
  20. Wu T. C., Lee W. A., Pizzorno M. C., Au W. C., Chan Y. J., Hruban R. H., Hutchins G. M., Hayward G. S. Localization of the human cytomegalovirus 2.7-kb major early beta-gene transcripts by RNA in situ hybridization in permissive and nonpermissive infections. Am J Pathol. 1992 Nov;141(5):1247–1254. [PMC free article] [PubMed] [Google Scholar]
  21. Yamashiroya H. M., Ghosh L., Yang R., Robertson A. L., Jr Herpesviridae in the coronary arteries and aorta of young trauma victims. Am J Pathol. 1988 Jan;130(1):71–79. [PMC free article] [PubMed] [Google Scholar]
  22. Yuhasz S. A., Dissette V. B., Cook M. L., Stevens J. G. Murine cytomegalovirus is present in both chronic active and latent states in persistently infected mice. Virology. 1994 Jul;202(1):272–280. doi: 10.1006/viro.1994.1343. [DOI] [PubMed] [Google Scholar]
  23. Zhang L. J., Hanff P., Rutherford C., Churchill W. H., Crumpacker C. S. Detection of human cytomegalovirus DNA, RNA, and antibody in normal donor blood. J Infect Dis. 1995 Apr;171(4):1002–1006. doi: 10.1093/infdis/171.4.1002. [DOI] [PubMed] [Google Scholar]
  24. van Lijnschoten G., Stals F., Evers J. L., Bruggeman C. A., Havenith M. H., Geraedts J. P. The presence of cytomegalovirus antigens in karyotyped abortions. Am J Reprod Immunol. 1994 Oct;32(3):211–220. doi: 10.1111/j.1600-0897.1994.tb01116.x. [DOI] [PubMed] [Google Scholar]
  25. van Loon A. M., Heessen F. W., van der Logt J. T., van der Veen J. Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus. J Clin Microbiol. 1981 Mar;13(3):416–422. doi: 10.1128/jcm.13.3.416-422.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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