Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1997 Feb;50(2):135–137. doi: 10.1136/jcp.50.2.135

Co-localisation of insulin-like growth factor II and the proliferation marker MIB1 in hepatocellular carcinoma cells.

T Sohda 1, Y Oka 1, K Iwata 1, J Gunn 1, S Kamimura 1, H Shijo 1, M Okumura 1, K Yun 1
PMCID: PMC499738  PMID: 9155694

Abstract

AIM: To assess the effect of insulin-like growth factor II (IGF-II) on proliferation of hepatocellular carcinoma (HCC) cells. METHODS: Expression of IGF-II mRNA and protein was detected in 10 archival HCC specimens using in situ hybridisation and immunohistochemistry, respectively. Expression of the Ki-67 antigen, a proliferation marker, was determined immunohistochemically on the same sections. RESULTS: Increased expression of IGF-II mRNA and protein was detected in five of the 10 HCCs in cells located at the periphery of tumour nests. The pattern of localisation of IGF-II was almost identical with that of Ki-67 antigen. CONCLUSIONS: Most of the Ki-67 antigen positive cells co-expressed IGF-II, suggesting that IGF-II may act as an autocrine or paracrine growth factor, and may play an important role in the development of HCC.

Full text

PDF

Images in this article

136Image
on p.136
136Image
on p.136

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. D'Errico A., Grigioni W. F., Fiorentino M., Baccarini P., Lamas E., De Mitri S., Gozzetti G., Mancini A. M., Brechot C. Expression of insulin-like growth factor II (IGF-II) in human hepatocellular carcinomas: an immunohistochemical study. Pathol Int. 1994 Feb;44(2):131–137. doi: 10.1111/j.1440-1827.1994.tb01697.x. [DOI] [PubMed] [Google Scholar]
  2. Grigioni W. F., D'Errico A., Bacci F., Gaudio M., Mazziotti A., Gozzetti G., Mancini A. M. Primary liver neoplasms: evaluation of proliferative index using MoAb Ki67. J Pathol. 1989 May;158(1):23–29. doi: 10.1002/path.1711580107. [DOI] [PubMed] [Google Scholar]
  3. Moses A. C., Nissley S. P., Short P. A., Rechler M. M., White R. M., Knight A. B., Higa O. Z. Increased levels of multiplication-stimulating activity, an insulin-like growth factor, in fetal rat serum. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3649–3653. doi: 10.1073/pnas.77.6.3649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nardone G., Romano M., Calabrò A., Pedone P. V., de Sio I., Persico M., Budillon G., Bruni C. B., Riccio A., Zarrilli R. Activation of fetal promoters of insulinlike growth factors II gene in hepatitis C virus-related chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Hepatology. 1996 Jun;23(6):1304–1312. doi: 10.1002/hep.510230602. [DOI] [PubMed] [Google Scholar]
  5. Rogler C. E., Yang D., Rossetti L., Donohoe J., Alt E., Chang C. J., Rosenfeld R., Neely K., Hintz R. Altered body composition and increased frequency of diverse malignancies in insulin-like growth factor-II transgenic mice. J Biol Chem. 1994 May 13;269(19):13779–13784. [PubMed] [Google Scholar]
  6. Sporn M. B., Roberts A. B. Autocrine growth factors and cancer. 1985 Feb 28-Mar 6Nature. 313(6005):745–747. doi: 10.1038/313745a0. [DOI] [PubMed] [Google Scholar]
  7. Sporn M. B., Todaro G. J. Autocrine secretion and malignant transformation of cells. N Engl J Med. 1980 Oct 9;303(15):878–880. doi: 10.1056/NEJM198010093031511. [DOI] [PubMed] [Google Scholar]
  8. Su Q., Liu Y. F., Zhang J. F., Zhang S. X., Li D. F., Yang J. J. Expression of insulin-like growth factor II in hepatitis B, cirrhosis and hepatocellular carcinoma: its relationship with hepatitis B virus antigen expression. Hepatology. 1994 Oct;20(4 Pt 1):788–799. doi: 10.1002/hep.1840200404. [DOI] [PubMed] [Google Scholar]
  9. Tashiro H., Iwasaki H., Kikuchi M., Ogata K., Okazaki M. Giant cell tumors of tendon sheath: a single and multiple immunostaining analysis. Pathol Int. 1995 Feb;45(2):147–155. doi: 10.1111/j.1440-1827.1995.tb03435.x. [DOI] [PubMed] [Google Scholar]
  10. Yun K. A new marker for rhabdomyosarcoma. Insulin-like growth factor II. Lab Invest. 1992 Nov;67(5):653–664. [PubMed] [Google Scholar]
  11. Yun K., Fidler A. E., Eccles M. R., Reeve A. E. Insulin-like growth factor II and WT1 transcript localization in human fetal kidney and Wilms' tumor. Cancer Res. 1993 Nov 1;53(21):5166–5171. [PubMed] [Google Scholar]
  12. d'Arville C. N., Nouri-Aria K. T., Johnson P., Williams R. Regulation of insulin-like growth factor II gene expression by hepatitis B virus in hepatocellular carcinoma. Hepatology. 1991 Feb;13(2):310–315. [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES