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. 1986 Apr;39(4):435–439. doi: 10.1136/jcp.39.4.435

Epithelial membrane antigen and cytokeratin expression by meningiomas: an immunohistological study.

J M Theaker, K C Gatter, M M Esiri, K A Fleming
PMCID: PMC499841  PMID: 2422220

Abstract

Thirteen meningiomas of varying light microscopic features were studied immunohistologically using a panel of monoclonal antibodies directed against epithelial, mesenchymal, and neural components. All 13 meningiomas showed expression of epithelial membrane antigen, vimentin, and S100 protein, as did normal meninges. Five of the 13 meningiomas also showed focal expression of cytokeratins, with double labelling showing expression of cytokeratins and vimentin by different cells. The cytokeratin expression was especially noticeable in cells surrounding the hyaline bodies of meningiomas. These results provide further evidence that meningiomas have features of both mesenchymal and epithelial tissues.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Budka H. Hyaline inclusions (Pseudopsammoma bodies) in meningiomas: immunocytochemical demonstration of epithel-like secretion of secretory component and immunoglobulins A and M. Acta Neuropathol. 1982;56(4):294–298. doi: 10.1007/BF00691261. [DOI] [PubMed] [Google Scholar]
  2. Churg A. Immunohistochemical staining for vimentin and keratin in malignant mesothelioma. Am J Surg Pathol. 1985 May;9(5):360–365. doi: 10.1097/00000478-198505000-00006. [DOI] [PubMed] [Google Scholar]
  3. Coakham H. B., Garson J. A., Allan P. M., Harper E. I., Brownell B., Kemshead J. T., Lane E. B. Immunohistological diagnosis of central nervous system tumours using a monoclonal antibody panel. J Clin Pathol. 1985 Feb;38(2):165–173. doi: 10.1136/jcp.38.2.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  5. Cordell J., Richardson T. C., Pulford K. A., Ghosh A. K., Gatter K. C., Heyderman E., Mason D. Y. Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer. 1985 Sep;52(3):347–354. doi: 10.1038/bjc.1985.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daffos F., Forestier F., Grangeot-Keros L., Capella Pavlovsky M., Lebon P., Chartier M., Pillot J. Prenatal diagnosis of congenital rubella. Lancet. 1984 Jul 7;2(8393):1–3. doi: 10.1016/s0140-6736(84)91993-7. [DOI] [PubMed] [Google Scholar]
  7. Frank E. H., Burge B. W., Liwnicz B. H., Lotspeich L. J., White J. C., Wechsler S. L., Mayfield F. H., Keller J. T. Cytokeratin provides a specific marker for human arachnoid cells grown in vitro. Exp Cell Res. 1983 Jul;146(2):371–376. doi: 10.1016/0014-4827(83)90138-6. [DOI] [PubMed] [Google Scholar]
  8. Miettinen M., Lehto V. P., Virtanen I. Antibodies to intermediate filament proteins in the diagnosis and classification of human tumors. Ultrastruct Pathol. 1984;7(2-3):83–107. doi: 10.3109/01913128409141467. [DOI] [PubMed] [Google Scholar]
  9. NAPOLITANO L., KYLE R., FISHER E. R. ULTRASTRUCTURE OF MENINGIOMAS AND THE DERIVATION AND NATURE OF THEIR CELLULAR COMPONENTS. Cancer. 1964 Feb;17:233–241. doi: 10.1002/1097-0142(196402)17:2<233::aid-cncr2820170213>3.0.co;2-n. [DOI] [PubMed] [Google Scholar]
  10. Nagle R. B., McDaniel K. M., Clark V. A., Payne C. M. The use of antikeratin antibodies in the diagnosis of human neoplasms. Am J Clin Pathol. 1983 Apr;79(4):458–466. doi: 10.1093/ajcp/79.4.458. [DOI] [PubMed] [Google Scholar]
  11. Nakamura Y., Becker L. E., Marks A. Distribution of immunoreactive S-100 protein in pediatric brain tumors. J Neuropathol Exp Neurol. 1983 Mar;42(2):136–145. doi: 10.1097/00005072-198303000-00003. [DOI] [PubMed] [Google Scholar]
  12. Ramaekers F. C., Puts J. J., Moesker O., Kant A., Huysmans A., Haag D., Jap P. H., Herman C. J., Vooijs G. P. Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview. Histochem J. 1983 Jul;15(7):691–713. doi: 10.1007/BF01002988. [DOI] [PubMed] [Google Scholar]
  13. Salisbury J. R., Isaacson P. G. Synovial sarcoma: an immunohistochemical study. J Pathol. 1985 Sep;147(1):49–57. doi: 10.1002/path.1711470107. [DOI] [PubMed] [Google Scholar]
  14. Schwechheimer K., Kartenbeck J., Moll R., Franke W. W. Vimentin filament-desmosome cytoskeleton of diverse types of human meningiomas. A distinctive diagnostic feature. Lab Invest. 1984 Nov;51(5):584–591. [PubMed] [Google Scholar]
  15. Sloane J. P., Ormerod M. G. Distribution of epithelial membrane antigen in normal and neoplastic tissues and it value in diagnostic tumor pathology. Cancer. 1981 Apr 1;47(7):1786–1795. doi: 10.1002/1097-0142(19810401)47:7<1786::aid-cncr2820470711>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  16. Vanstapel M. J., Peeters B., Cordell J., Heyns W., De Wolf-Peeters C., Desmet V., Mason D. Production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain S-100 protein. Lab Invest. 1985 Feb;52(2):232–238. [PubMed] [Google Scholar]
  17. Viac J., Reano A., Brochier J., Staquet M. J., Thivolet J. Reactivity pattern of a monoclonal antikeratin antibody (KL1). J Invest Dermatol. 1983 Oct;81(4):351–354. doi: 10.1111/1523-1747.ep12519941. [DOI] [PubMed] [Google Scholar]
  18. Whitaker D., Shilkin K. B. Diagnosis of pleural malignant mesothelioma in life--a practical approach. J Pathol. 1984 Jul;143(3):147–175. doi: 10.1002/path.1711430303. [DOI] [PubMed] [Google Scholar]

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