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. 1986 Jul;39(7):736–741. doi: 10.1136/jcp.39.7.736

Study of childhood renal tumours using peroxidase conjugated lectins.

S Kumar, T Carr, H B Marsden, P H Morris-Jones
PMCID: PMC500034  PMID: 3016034

Abstract

Six peroxidase conjugated lectins were used to compare their ability to bind to formalin fixed paraffin embedded tissue sections of childhood renal tumours (Wilms' tumour, mesoblastic nephroma, renal carcinoma, rhabdoid renal tumour, and bone metastasising renal tumour of childhood (BMRTC) with fetal and normal children's kidney. Lectins were found to be helpful in the differential diagnosis of renal tumours. Another important finding was that the mesenchyme of renal tumours showed differences in its reactivity among various types of kidney tumours. The results of lectin binding were not helpful in establishing the origin of kidney tumours.

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Selected References

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  1. Burtin P., Gendron M. C. A tumor-associated antigen in human nephroblastomas. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2051–2054. doi: 10.1073/pnas.70.7.2051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Faraggiana T., Malchiodi F., Prado A., Churg J. Lectin-peroxidase conjugate reactivity in normal human kidney. J Histochem Cytochem. 1982 May;30(5):451–458. doi: 10.1177/30.5.7077075. [DOI] [PubMed] [Google Scholar]
  3. Gallagher J. T. Carbohydrate-binding properties of lectins: a possible approach to lectin nomenclature and classification. Review. Biosci Rep. 1984 Aug;4(8):621–632. doi: 10.1007/BF01121015. [DOI] [PubMed] [Google Scholar]
  4. Haas J. E., Bonadio J. F., Beckwith J. B. Clear cell sarcoma of the kidney with emphasis on ultrastructural studies. Cancer. 1984 Dec 15;54(12):2978–2987. doi: 10.1002/1097-0142(19841215)54:12<2978::aid-cncr2820541228>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  5. Holthöfer H. Lectin binding sites in kidney. A comparative study of 14 animal species. J Histochem Cytochem. 1983 Apr;31(4):531–537. doi: 10.1177/31.4.6827083. [DOI] [PubMed] [Google Scholar]
  6. Holthöfer H., Virtanen I., Pettersson E., Törnroth T., Alfthan O., Linder E., Miettinen A. Lectins as fluorescence microscopic markers for saccharides in the human kidney. Lab Invest. 1981 Nov;45(5):391–399. [PubMed] [Google Scholar]
  7. Kumar S., Carr T., Marsden H. B., Calabuig-Crespo M. C. Study of childhood renal tumours using antisera to fibronectin, laminin, and epithelial membrane antigen. J Clin Pathol. 1986 Jan;39(1):51–57. doi: 10.1136/jcp.39.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kumar S., Jasani B., Hunt J. S., Moffat D. B., Asscher A. W. A system for accurate immunolocalization of Tamm-Horsfall protein in renal biopsies. Histochem J. 1985 Nov;17(11):1251–1258. doi: 10.1007/BF01002506. [DOI] [PubMed] [Google Scholar]
  9. Kumar S., Marsden H. B., Calabuig M. C. Childhood kidney tumours: in vitro studies and natural history. Virchows Arch A Pathol Anat Histopathol. 1984;405(1):95–111. doi: 10.1007/BF00694928. [DOI] [PubMed] [Google Scholar]
  10. Kumar S., Marsden H. B., Carr T., Kodet R. Mesoblastic nephroma contains fibronectin but lacks laminin. J Clin Pathol. 1985 May;38(5):507–511. doi: 10.1136/jcp.38.5.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kumar S., Marsden H. B., Kumar P. A study of childhood renal tumours using xenogeneic antiserum. Br J Urol. 1980 Aug;52(4):245–252. doi: 10.1111/j.1464-410x.1980.tb08911.x. [DOI] [PubMed] [Google Scholar]
  12. Marsden H. B., Lawler W., Carr T., Kumar S. A scoring system for Wilms' tumour: pathological study of the second Medical Research Council (MRC) trial. Int J Cancer. 1984 Mar 15;33(3):365–368. doi: 10.1002/ijc.2910330314. [DOI] [PubMed] [Google Scholar]
  13. Marsden H. B., Newton W. A., Jr New look at mesoblastic nephroma. J Clin Pathol. 1986 May;39(5):508–513. doi: 10.1136/jcp.39.5.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Raedler A., Boehle A., Otto U., Raedler E. Differences of glycoconjugates exposed on hypernephroma and normal kidney cells. J Urol. 1982 Nov;128(5):1109–1113. doi: 10.1016/s0022-5347(17)53355-8. [DOI] [PubMed] [Google Scholar]
  15. Tannenbaum M. Ultrastructural pathology of human renal cell tumors. Pathol Annu. 1971;6:249–277. [PubMed] [Google Scholar]
  16. Vogel A. M., Gown A. M., Caughlan J., Haas J. E., Beckwith J. B. Rhabdoid tumors of the kidney contain mesenchymal specific and epithelial specific intermediate filament proteins. Lab Invest. 1984 Feb;50(2):232–238. [PubMed] [Google Scholar]
  17. Wallace A. C., Nairn R. C. Renal tubular antigens in kidney tumors. Cancer. 1972 Apr;29(4):977–981. doi: 10.1002/1097-0142(197204)29:4<977::aid-cncr2820290444>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  18. Wigger H. J. Fetal hamartoma of kidney. A benign, symptomatic, congenital tumor, not a form of Wilms' tumor. Am J Clin Pathol. 1969 Mar;51(3):323–337. doi: 10.1093/ajcp/51.3.323. [DOI] [PubMed] [Google Scholar]

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