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. 2016 Jun 9;65(9):2810–2815. doi: 10.2337/db15-1666

Figure 2.

Figure 2

The activity of human PTF1A-P191T in transfected cells. The reporter plasmid 3Rbpjl.Ela1p.luc was cotransfected with expression plasmids for wild-type (w.t.) PTF1A, PTF1A-P191T, or an insertless vector into HEK293 cells. Three different amounts of each expression plasmid were used to determine whether response was proportional to plasmid quantity and, therefore, that the proteins produced were not in excess. Four independent transfections were analyzed for 10 and 5 ng and two for 2.5 ng. Error bars are SD. The asterisks indicate P values <0.05 for each pairwise comparison.