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. 1986 Sep;39(9):993–997. doi: 10.1136/jcp.39.9.993

High proliferative activity of Reed Sternberg associated antigen Ki-1 positive cells in normal lymphoid tissue.

J Gerdes, R Schwarting, H Stein
PMCID: PMC500199  PMID: 3020097

Abstract

An improved immunoenzymatic double labelling method was developed, which simultaneously shows the Ki-1 membrane antigen and the nuclear proliferation associated antigen, defined by monoclonal antibody Ki-67. This new approach permits in situ discrimination of cells that are proliferating or not with a particular membrane antigen. Most Ki-1 positive cells in normal lymphoid tissue also express the Ki-67 nuclear antigen and thus appear to be proliferating. As proliferating cells are more susceptible to malignant transformation than quiescent cells, it is possible that the small number of normal Ki-1 positive cells might, none the less, cause a large proportion of all lymphomas in man.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boecker W. R., Hossfeld D. K., Gallmeier W. M., Schmidt C. G. Clonal growth of Hodgkin cells. Nature. 1975 Nov 20;258(5532):235–236. doi: 10.1038/258235a0. [DOI] [PubMed] [Google Scholar]
  2. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  3. Curran R. C., Jones E. L. Hodgkin's disease: an immunohistochemical and histological study. J Pathol. 1978 May;125(1):39–51. doi: 10.1002/path.1711250107. [DOI] [PubMed] [Google Scholar]
  4. Diehl V., Kirchner H. H., Burrichter H., Stein H., Fonatsch C., Gerdes J., Schaadt M., Heit W., Uchanska-Ziegler B., Ziegler A. Characteristics of Hodgkin's disease-derived cell lines. Cancer Treat Rep. 1982 Apr;66(4):615–632. [PubMed] [Google Scholar]
  5. Fisher R. I., Bostick-Bruton F., Sauder D. N., Scala G., Diehl V. Neoplastic cells obtained from Hodgkin's disease are potent stimulators of human primary mixed lymphocyte cultures. J Immunol. 1983 Jun;130(6):2666–2670. [PubMed] [Google Scholar]
  6. Garvin A. J., Spicer S. S., Parmley R. T., Munster A. M. Immunohistochemical demonstration of IgG in Reed-Sternberg and other cells in Hodgkin's disease. J Exp Med. 1974 May 1;139(5):1077–1083. doi: 10.1084/jem.139.5.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerdes J., Lemke H., Baisch H., Wacker H. H., Schwab U., Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710–1715. [PubMed] [Google Scholar]
  8. Gerdes J., Schwab U., Lemke H., Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13–20. doi: 10.1002/ijc.2910310104. [DOI] [PubMed] [Google Scholar]
  9. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  10. Hansmann M. L., Kaiserling E. Electron-microscopic aspects of Hodgkin's disease. J Cancer Res Clin Oncol. 1981;101(1):135–148. doi: 10.1007/BF00405074. [DOI] [PubMed] [Google Scholar]
  11. Isaacson P. Immunochemical demonstration of J chain: a marker of B-cell malignancy. J Clin Pathol. 1979 Aug;32(8):802–807. doi: 10.1136/jcp.32.8.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kadin M. E., Newcom S. R., Gold S. B., Stites D. P. Letter: Origin of Hodgkin's cell. Lancet. 1974 Jul 20;2(7873):167–168. doi: 10.1016/s0140-6736(74)91602-x. [DOI] [PubMed] [Google Scholar]
  13. Kadin M. E., Stites D. P., Levy R., Warnke R. Exogenous immunoglobulin and the macrophage origin of Reed-Sternberg cells in Hodgkin's disease. N Engl J Med. 1978 Nov 30;299(22):1208–1214. doi: 10.1056/NEJM197811302992203. [DOI] [PubMed] [Google Scholar]
  14. Kaplan H. S., Gartner S. "Sternberg-reed" giant cells of Hodgkin's Disease: cultivation in vitro, heterotransplantation, and characterization as neoplastic macrophages. Int J Cancer. 1977 Apr 15;19(4):511–525. doi: 10.1002/ijc.2910190412. [DOI] [PubMed] [Google Scholar]
  15. Landaas T. O., Godal T., Halvorsen T. B. Characterization of immunoglobulins in Hodgkin cells. Int J Cancer. 1977 Nov 15;20(5):717–722. doi: 10.1002/ijc.2910200511. [DOI] [PubMed] [Google Scholar]
  16. Lukes R. J. Criteria for involvement of lymph node, bone marrow, spleen, and liver in Hodgkin's disease. Cancer Res. 1971 Nov;31(11):1755–1767. [PubMed] [Google Scholar]
  17. Schwab U., Stein H., Gerdes J., Lemke H., Kirchner H., Schaadt M., Diehl V. Production of a monoclonal antibody specific for Hodgkin and Sternberg-Reed cells of Hodgkin's disease and a subset of normal lymphoid cells. Nature. 1982 Sep 2;299(5878):65–67. doi: 10.1038/299065a0. [DOI] [PubMed] [Google Scholar]
  18. Stein H., Gerdes J., Schwab U., Lemke H., Diehl V., Mason D. Y., Bartels H., Ziegler A. Evidence for the detection of the normal counterpart of Hodgkin and Sternberg-Reed cells. Hematol Oncol. 1983 Jan-Mar;1(1):21–29. doi: 10.1002/hon.2900010105. [DOI] [PubMed] [Google Scholar]
  19. Stein H., Gerdes J., Schwab U., Lemke H., Mason D. Y., Ziegler A., Schienle W., Diehl V. Identification of Hodgkin and Sternberg-reed cells as a unique cell type derived from a newly-detected small-cell population. Int J Cancer. 1982 Oct 15;30(4):445–459. doi: 10.1002/ijc.2910300411. [DOI] [PubMed] [Google Scholar]
  20. Stein H., Mason D. Y., Gerdes J., O'Connor N., Wainscoat J., Pallesen G., Gatter K., Falini B., Delsol G., Lemke H. The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood. 1985 Oct;66(4):848–858. [PubMed] [Google Scholar]
  21. Taylor C. R. An immunohistological study of follicular lymphoma, reticulum cell sarcoma and Hodgkin's disease. Eur J Cancer. 1976 Jan;12(1):61–75. doi: 10.1016/0014-2964(76)90125-0. [DOI] [PubMed] [Google Scholar]
  22. Uchiyama T., Broder S., Waldmann T. A. A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. J Immunol. 1981 Apr;126(4):1393–1397. [PubMed] [Google Scholar]

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