Figure 4. HIV infection induced migration of central memory CD4⁺ T cell chemotaxis.

Supernatants collected from cultured RTECs at day 3 post HIV infection (cell-associated infection) served as chemoattractants. PHA activated (A) or non-activated (B) CD4⁺ T cells were placed at the top chamber. Cell migration was terminated after incubation at 37°C for three hours. Migrated cells were washed once with PBS and stained with a panel of cell marker antibodies T_CM (CCR7⁺CD62L⁺CD45RO⁺CD45RA⁻) and analyzed by flow cytometry using FlowJo software.