Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1986 Oct;39(10):1124–1129. doi: 10.1136/jcp.39.10.1124

Development of enzyme linked immunosorbent assay (ELISA) to detect antibodies to Pseudomonas aeruginosa cell surface antigens in sera of patients with cystic fibrosis.

M M Brett, A T Ghoneim, J M Littlewood, M S Losowsky
PMCID: PMC500235  PMID: 3097080

Abstract

An enzyme linked immunosorbent assay (ELISA) to measure free serum IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis was developed. Seven strains of P aeruginosa cells, treated with glutaraldehyde and representing the most commonly isolated serotypes in our cystic fibrosis unit, were used. The specificity of the test was confirmed by the absence of cross reacting antibodies to other Gram negative bacteria. The results showed differences in the titres of antibodies at different stages of P aeruginosa infection. Because of its reproducibility, specificity, and sensitivity these preliminary results suggest that this test may be of value in monitoring the progress of P aeruginosa infection in patients with cystic fibrosis.

Full text

PDF
1124

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borowski R. S., Stock L. M., Schiller N. L. Development of an enzyme-linked immunosorbent assay for studying Pseudomonas aeruginosa cell surface antigens. J Clin Microbiol. 1984 Jun;19(6):736–741. doi: 10.1128/jcm.19.6.736-741.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cost K. M., West C. S., Brinson D., Polk H. C., Jr Measurement of human antibody activity against Escherichia coli and Pseudomonas aeruginosa using formalin treated whole organisms in an ELISA technique. J Immunoassay. 1985;6(1-2):23–43. doi: 10.1080/01971528508063019. [DOI] [PubMed] [Google Scholar]
  3. Danneman P. J., Michael J. G. Reaginic antibody production to protein antigens of Escherichia coli and Pseudomonas aeruginosa by mice. Infect Immun. 1976 Sep;14(3):694–702. doi: 10.1128/iai.14.3.694-702.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GIBSON L. E., COOKE R. E. A test for concentration of electrolytes in sweat in cystic fibrosis of the pancreas utilizing pilocarpine by iontophoresis. Pediatrics. 1959 Mar;23(3):545–549. [PubMed] [Google Scholar]
  5. Gilleland H. E., Jr, Parker M. G., Matthews J. M., Berg R. D. Use of a purified outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine in mice. Infect Immun. 1984 Apr;44(1):49–54. doi: 10.1128/iai.44.1.49-54.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hancock R. E., Mouat E. C., Speert D. P. Quantitation and identification of antibodies to outer-membrane proteins of Pseudomonas aeruginosa in sera of patients with cystic fibrosis. J Infect Dis. 1984 Feb;149(2):220–226. doi: 10.1093/infdis/149.2.220. [DOI] [PubMed] [Google Scholar]
  7. Hancock R. E., Mouat E. C., Speert D. P. Quantitation and identification of antibodies to outer-membrane proteins of Pseudomonas aeruginosa in sera of patients with cystic fibrosis. J Infect Dis. 1984 Feb;149(2):220–226. doi: 10.1093/infdis/149.2.220. [DOI] [PubMed] [Google Scholar]
  8. Hancock R. E., Wieczorek A. A., Mutharia L. M., Poole K. Monoclonal antibodies against Pseudomonas aeruginosa outer membrane antigens: isolation and characterization. Infect Immun. 1982 Jul;37(1):166–171. doi: 10.1128/iai.37.1.166-171.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hedstrom R. C., Pavlovskis O. R., Galloway D. R. Antibody response of infected mice to outer membrane proteins of Pseudomonas aeruginosa. Infect Immun. 1984 Jan;43(1):49–53. doi: 10.1128/iai.43.1.49-53.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kohler R. B., Wheat L. J., White A. Rapid diagnosis of Pseudomonas aeruginosa urinary tract infections by radioimmunoassay. J Clin Microbiol. 1979 Feb;9(2):253–258. doi: 10.1128/jcm.9.2.253-258.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lam J. S., Mutharia L. M., Hancock R. E., Høiby N., Lam K., Baek L., Costerton J. W. Immunogenicity of Pseudomonas aeruginosa outer membrane antigens examined by crossed immunoelectrophoresis. Infect Immun. 1983 Oct;42(1):88–98. doi: 10.1128/iai.42.1.88-98.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peters K., Richards F. M. Chemical cross-linking: reagents and problems in studies of membrane structure. Annu Rev Biochem. 1977;46:523–551. doi: 10.1146/annurev.bi.46.070177.002515. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES