Abstract
Of the first 128 patients to receive either heart or heart and lung transplants at Papworth Hospital, four developed Toxoplasma gondii infections acquired from the donor heart and two died. Six patients had passively acquired T gondii antibody as a result of blood transfusions around the time of transplantation. Eight patients developed antibodies against T gondii, which were detectable by changes in the latex agglutination test titres but not by those of the dye test. These false positive latex agglutination reactions occurred simultaneously with cytomegalovirus infection and were associated with the IgM serum fraction in the patients' sera. These reactions were not associated with cytomegalovirus specific IgM, Paul-Bunnell antibody, nor detectable rheumatoid factor. These findings emphasise the need for T gondii dye test confirmation of latex agglutination test titre rises in heart transplant recipients.
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- BRADSTREET C. M., TAYLOR C. E. Technique of complementfixation test applicable to the diagnosis of virus diseases. Mon Bull Minist Health Public Health Lab Serv. 1962 May;21:96–104. [PubMed] [Google Scholar]
- Hakim M., Esmore D., Wallwork J., English T. A., Wreghitt T. Toxoplasmosis in cardiac transplantation. Br Med J (Clin Res Ed) 1986 Apr 26;292(6528):1108–1108. doi: 10.1136/bmj.292.6528.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hakim M., Wreghitt T. G., English T. A., Stovin P. G., Cory-Pearce R., Wallwork J. Significance of donor transmitted disease in cardiac transplantation. J Heart Transplant. 1985 May;4(3):302–306. [PubMed] [Google Scholar]
- Hunter D., Chadwick P., Balfour A. H., Bridges J. B. Examination of ovine foetal fluid for antibodies to Toxoplasma gondii by the dye test and an indirect immunofluorescence test specific for IgM. Br Vet J. 1982 Jan-Feb;138(1):29–34. doi: 10.1016/s0007-1935(17)31186-7. [DOI] [PubMed] [Google Scholar]
- Luft B. J., Naot Y., Araujo F. G., Stinson E. B., Remington J. S. Primary and reactivated toxoplasma infection in patients with cardiac transplants. Clinical spectrum and problems in diagnosis in a defined population. Ann Intern Med. 1983 Jul;99(1):27–31. doi: 10.7326/0003-4819-99-1-27. [DOI] [PubMed] [Google Scholar]
- McGregor C. G., Fleck D. G., Nagington J., Stovin P. G., Cory-Pearce R., English T. A. Disseminated toxoplasmosis in cardiac transplantation. J Clin Pathol. 1984 Jan;37(1):74–77. doi: 10.1136/jcp.37.1.74. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nagington J., Martin A. L. Toxoplasmosis and heart transplantation. Lancet. 1983 Sep 17;2(8351):679–679. doi: 10.1016/s0140-6736(83)92553-9. [DOI] [PubMed] [Google Scholar]
- Naot Y., Luft B. J., Remington J. S. False-positive serological tests in heart transplant recipients. Lancet. 1981 Sep 12;2(8246):590–591. doi: 10.1016/s0140-6736(81)90986-7. [DOI] [PubMed] [Google Scholar]
- Payne R. A., Isaac M., Francis J. M. Enzyme-linked immunosorbent assay (ELISA) using antibody class capture for the detection of antitoxoplasma IgM. J Clin Pathol. 1982 Aug;35(8):892–896. doi: 10.1136/jcp.35.8.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stinson E. B., Bieber C. P., Griepp R. B., Clark D. A., Shumway N. E., Remington J. S. Infectious complications after cardiac transplantation in man. Ann Intern Med. 1971 Jan;74(1):22–36. doi: 10.7326/0003-4819-74-1-22. [DOI] [PubMed] [Google Scholar]
- Wreghitt T. G., Hicks J., Gray J. J., O'Connor C. Development of a competitive enzyme-linked immunosorbent assay for detecting cytomegalovirus antibody. J Med Virol. 1986 Feb;18(2):119–129. doi: 10.1002/jmv.1890180204. [DOI] [PubMed] [Google Scholar]