Abstract
AIMS: To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi. METHODS: A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used. RESULTS: Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens. CONCLUSIONS: The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.
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- Chart H., Montgomery S., Rowe B. A rapid immunoblotting procedure for detecting serum antibodies to the lipopolysaccharide of Escherichia coli O157. Lett Appl Microbiol. 1994 Feb;18(2):100–101. doi: 10.1111/j.1472-765x.1994.tb00815.x. [DOI] [PubMed] [Google Scholar]
- FELIX A. Standardization of diagnostic agglutination tests; typhoid and paratyphoid A and B fevers. Bull World Health Organ. 1950;2(4):643–649. [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Sippel J. E., Hanafy H. M., Diab A. S., Prato C., Arroyo R. Serodiagnosis of typhoid fever in paediatric patients by anti-LPS ELISA. Trans R Soc Trop Med Hyg. 1987;81(6):1022–1026. doi: 10.1016/0035-9203(87)90386-5. [DOI] [PubMed] [Google Scholar]
- Tsai C. M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem. 1982 Jan 1;119(1):115–119. doi: 10.1016/0003-2697(82)90673-x. [DOI] [PubMed] [Google Scholar]