Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1996 Feb;49(2):107–111. doi: 10.1136/jcp.49.2.107

Detection of Helicobacter pylori in gastric biopsy and resection specimens.

M Ashton-Key 1, T C Diss 1, P G Isaacson 1
PMCID: PMC500340  PMID: 8655673

Abstract

AIMS: To compare the sensitivity of detecting Helicobacter pylori in gastric biopsy and resection specimens using tinctorial and silver impregnation stains, immunohistochemistry and the polymerase chain reaction (PCR). METHODS: Formalin fixed, paraffin wax embedded tissue from 33 gastric biopsy specimens (26 showing chronic gastritis and seven showing low grade mucosa associated lymphoid tissue (MALT) lymphoma) together with blocks of uninvolved mucosa from gastrectomy specimens for MALT lymphoma (five cases) were studied. Consecutive sections were stained using haematoxylin and eosin, Giemsa, the Warthin-Starry silver stain, and a polyclonal antibody directed against H pylori using an immunoperoxidase technique following heat induced antigen retrieval. PCR analysis of DNA extracted from a further section was carried out using primers which amplified a 411 base pair fragment of the urease A gene. RESULTS: H pylori was detected in 14 (37%) sections stained with haematoxylin and eosin, 21 (55%) with Giemsa, 23 (61%) with Warthin-Starry, and 25 (66%) stained with the antibody. Seventeen (45%) cases were positive on PCR. Immunohistochemistry was positive in all cases in which H pylori was detected by other methods. CONCLUSION: Immunohistochemistry using an immunoperoxidase technique following heat induced antigen retrieval for detecting H pylori in gastric biopsy and resection specimens is highly sensitive and easy to use.

Full text

PDF
107

Images in this article

108Image
on p.108
109Image
on p.109
109Image
on p.109

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bashir M. S., Lewis F. A., Quirke P., Lee A., Dixon M. F. In situ hybridisation for the identification of Helicobacter pylori in paraffin wax embedded tissue. J Clin Pathol. 1994 Sep;47(9):862–864. doi: 10.1136/jcp.47.9.862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bode G., Mauch F., Malfertheiner P. The coccoid forms of Helicobacter pylori. Criteria for their viability. Epidemiol Infect. 1993 Dec;111(3):483–490. doi: 10.1017/s0950268800057216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cartun R. W., Kryzmowski G. A., Pedersen C. A., Morin S. G., Van Kruiningen H. J., Berman M. M. Immunocytochemical identification of Helicobacter pylori in formalin-fixed gastric biopsies. Mod Pathol. 1991 Jul;4(4):498–502. [PubMed] [Google Scholar]
  4. Clayton C. L., Kleanthous H., Coates P. J., Morgan D. D., Tabaqchali S. Sensitive detection of Helicobacter pylori by using polymerase chain reaction. J Clin Microbiol. 1992 Jan;30(1):192–200. doi: 10.1128/jcm.30.1.192-200.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clayton C., Kleanthous K., Tabaqchali S. Detection and identification of Helicobacter pylori by the polymerase chain reaction. J Clin Pathol. 1991 Jun;44(6):515–516. doi: 10.1136/jcp.44.6.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  7. Diss T. C., Pan L., Peng H., Wotherspoon A. C., Isaacson P. G. Sources of DNA for detecting B cell monoclonality using PCR. J Clin Pathol. 1994 Jun;47(6):493–496. doi: 10.1136/jcp.47.6.493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fabre R., Sobhani I., Laurent-Puig P., Hedef N., Yazigi N., Vissuzaine C., Rodde I., Potet F., Mignon M., Etienne J. P. Polymerase chain reaction assay for the detection of Helicobacter pylori in gastric biopsy specimens: comparison with culture, rapid urease test, and histopathological tests. Gut. 1994 Jul;35(7):905–908. doi: 10.1136/gut.35.7.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goodwin C. S., Armstrong J. A., Marshall B. J. Campylobacter pyloridis, gastritis, and peptic ulceration. J Clin Pathol. 1986 Apr;39(4):353–365. doi: 10.1136/jcp.39.4.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goodwin C. S., Blincow E. D., Warren J. R., Waters T. E., Sanderson C. R., Easton L. Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol. 1985 Oct;38(10):1127–1131. doi: 10.1136/jcp.38.10.1127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Graham D. Y., Klein P. D., Evans D. J., Jr, Evans D. G., Alpert L. C., Opekun A. R., Boutton T. W. Campylobacter pylori detected noninvasively by the 13C-urea breath test. Lancet. 1987 May 23;1(8543):1174–1177. doi: 10.1016/s0140-6736(87)92145-3. [DOI] [PubMed] [Google Scholar]
  12. Kolts B. E., Joseph B., Achem S. R., Bianchi T., Monteiro C. Helicobacter pylori detection: a quality and cost analysis. Am J Gastroenterol. 1993 May;88(5):650–655. [PubMed] [Google Scholar]
  13. Loffeld R. J., Stobberingh E., Flendrig J. A., Arends J. W. Helicobacter pylori in gastric biopsy specimens. Comparison of culture, modified giemsa stain, and immunohistochemistry. A retrospective study. J Pathol. 1991 Sep;165(1):69–73. doi: 10.1002/path.1711650111. [DOI] [PubMed] [Google Scholar]
  14. Marshall B. J., Warren J. R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984 Jun 16;1(8390):1311–1315. doi: 10.1016/s0140-6736(84)91816-6. [DOI] [PubMed] [Google Scholar]
  15. McNulty C. A., Dent J. C., Uff J. S., Gear M. W., Wilkinson S. P. Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients. Gut. 1989 Aug;30(8):1058–1062. doi: 10.1136/gut.30.8.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miller N. M., Sathar M. A., Naran A. D., van den Ende J., Simjee A. E., Manion G. Evaluation of various laboratory techniques to diagnose Helicobacter pylori in patients with upper gastro-intestinal tract symptoms. S Afr Med J. 1991 Dec 7;80(11-12):575–578. [PubMed] [Google Scholar]
  17. Negrini R., Lisato L., Cavazzini L., Maini P., Gullini S., Basso O., Lanza G., Jr, Garofalo M., Nenci I. Monoclonal antibodies for specific immunoperoxidase detection of Campylobacter pylori. Gastroenterology. 1989 Feb;96(2 Pt 1):414–420. doi: 10.1016/0016-5085(89)91565-5. [DOI] [PubMed] [Google Scholar]
  18. Norton A. J., Jordan S., Yeomans P. Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J Pathol. 1994 Aug;173(4):371–379. doi: 10.1002/path.1711730413. [DOI] [PubMed] [Google Scholar]
  19. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991 Oct 17;325(16):1127–1131. doi: 10.1056/NEJM199110173251603. [DOI] [PubMed] [Google Scholar]
  20. Van den Berg F. M., Zijlmans H., Langenberg W., Rauws E., Schipper M. Detection of Campylobacter pylori in stomach tissue by DNA in situ hybridisation. J Clin Pathol. 1989 Sep;42(9):995–1000. doi: 10.1136/jcp.42.9.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weiss J., Mecca J., da Silva E., Gassner D. Comparison of PCR and other diagnostic techniques for detection of Helicobacter pylori infection in dyspeptic patients. J Clin Microbiol. 1994 Jul;32(7):1663–1668. doi: 10.1128/jcm.32.7.1663-1668.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wotherspoon A. C., Doglioni C., Diss T. C., Pan L., Moschini A., de Boni M., Isaacson P. G. Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet. 1993 Sep 4;342(8871):575–577. doi: 10.1016/0140-6736(93)91409-f. [DOI] [PubMed] [Google Scholar]
  23. Wotherspoon A. C., Ortiz-Hidalgo C., Falzon M. R., Isaacson P. G. Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. Lancet. 1991 Nov 9;338(8776):1175–1176. doi: 10.1016/0140-6736(91)92035-z. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES