Skip to main content
. 2015 Aug;100(8):1051–1057. doi: 10.3324/haematol.2014.111989

Figure 3.

Figure 3.

Cellular consequences of BRCC3 knockdown. (A) Immunoblot analysis of Brcc3 levels in LSK cells at 96 h after infection with lentivirus carrying Brcc3 -specific or control (NC) shRNA. (B) Brcc3 knockdown significantly increased colony formation capability of murine lineage− Sca-1+c-kit+ (LSK) cells. Columns correspond to mean ± SD of colony numbers formed by 5000 puromycin-resistant murine LSK cells in the presence of SCF, IL-3, and IL-6 at 48 h after infection with lentivirus carrying Brcc3-specific or control shRNA and subsequent selection with puromycin. (C) Wright-Giemsa staining of LSK cells in the presence of SCF, IL-3, and IL-6 at 7 days after infection with lentivirus carrying Brcc3-specific or control shRNA (X200).