Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1996 Apr;49(4):309–312. doi: 10.1136/jcp.49.4.309

Parietal cells in the duodenal bulb and their relation to Helicobacter pylori infection.

A W Harris 1, M M Walker 1, A Smolka 1, J M Waller 1, J H Baron 1, J J Misiewicz 1
PMCID: PMC500457  PMID: 8655707

Abstract

AIM: To investigate the prevalence, and relation to Helicobacter pylori, of parietal cells in the duodenal bulb using a monoclonal antibody directed against H+,K(+)-ATPase (HK12.18). METHODS: Twenty six patients with duodenal ulcer disease and 16 healthy controls were studied. H pylori status was determined by gastric histology and culture and by the 13C-urea breath test. Four biopsy specimens were taken from the duodenal bulb and stained with HK12.18. The presence/absence and number of parietal cells in the duodenal bulb were assessed blindly by a histopathologist. RESULTS: The overall prevalence of parietal cells in the duodenal bulb was 31% (13/42) and was similar in patients with duodenal ulcer and in controls, and in H pylori positive and negative subjects. The median (range) number of parietal cells in the duodenal bulb was 7.5 (4-20) parietal cells/subject, and was similar in all four groups. CONCLUSIONS: The prevalence of parietal cells in the duodenal bulb (31%) is notably higher than previously reported in endoscopic studies, and is in keeping with reports from studies on necropsy/operative specimens. There was no difference in the prevalence or number of parietal cells in the duodenal bulb between patients with duodenal ulcer and controls, regardless of H pylori status. These findings suggest that parietal cells in the duodenal bulb do not contribute to the pathogenesis of duodenal ulcer.

Full text

PDF
309

Images in this article

310Image
on p.310
310Image
on p.310

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COX A. J. Stomach size and its relation to chronic peptic ulcer. AMA Arch Pathol. 1952 Nov;54(5):407–422. [PubMed] [Google Scholar]
  2. Carrick J., Lee A., Hazell S., Ralston M., Daskalopoulos G. Campylobacter pylori, duodenal ulcer, and gastric metaplasia: possible role of functional heterotopic tissue in ulcerogenesis. Gut. 1989 Jun;30(6):790–797. doi: 10.1136/gut.30.6.790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hoedemaeker P. J. Heterotopic gastric mucosa in the duodenum. Digestion. 1970;3(3):165–173. doi: 10.1159/000197027. [DOI] [PubMed] [Google Scholar]
  4. Johansen A., Hansen O. H. Heterotopic gastric epithelium in the duodenum and its correlation to gastric disease and acid level. Acta Pathol Microbiol Scand A. 1973 Sep;81(5):676–680. doi: 10.1111/j.1699-0463.1973.tb03560.x. [DOI] [PubMed] [Google Scholar]
  5. Karlsson F. A., Burman P., Löf L., Mårdh S. Major parietal cell antigen in autoimmune gastritis with pernicious anemia is the acid-producing H+,K+-adenosine triphosphatase of the stomach. J Clin Invest. 1988 Feb;81(2):475–479. doi: 10.1172/JCI113344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kelly E. J., Lagopoulos M., Primrose J. N. Immunocytochemical localisation of parietal cells and G cells in the developing human stomach. Gut. 1993 Aug;34(8):1057–1059. doi: 10.1136/gut.34.8.1057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kelly E. J., Lagopoulos M., Primrose J. N. Immunocytochemical localisation of parietal cells and G cells in the developing human stomach. Gut. 1993 Aug;34(8):1057–1059. doi: 10.1136/gut.34.8.1057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lawson H. H. Definition of gastroduodenal junction in healthy subjects. J Clin Pathol. 1988 Apr;41(4):393–396. doi: 10.1136/jcp.41.4.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leela K., Kanagasuntheram R. A microscopic study of the human pyloro-duodenal junction and proximal duodenum. Acta Anat (Basel) 1968;71(1):1–12. doi: 10.1159/000143164. [DOI] [PubMed] [Google Scholar]
  10. Logan R. P., Polson R. J., Misiewicz J. J., Rao G., Karim N. Q., Newell D., Johnson P., Wadsworth J., Walker M. M., Baron J. H. Simplified single sample 13Carbon urea breath test for Helicobacter pylori: comparison with histology, culture, and ELISA serology. Gut. 1991 Dec;32(12):1461–1464. doi: 10.1136/gut.32.12.1461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shousha S., Spiller R. C., Parkins R. A. The endoscopically abnormal duodenum in patients with dyspepsia: biopsy findings in 60 cases. Histopathology. 1983 Jan;7(1):23–34. doi: 10.1111/j.1365-2559.1983.tb02213.x. [DOI] [PubMed] [Google Scholar]
  12. Smolka A., Helander H. F., Sachs G. Monoclonal antibodies against gastric H+ + K+ ATPase. Am J Physiol. 1983 Oct;245(4):G589–G596. doi: 10.1152/ajpgi.1983.245.4.G589. [DOI] [PubMed] [Google Scholar]
  13. Soroka C. J., Chew C. S., Hanzel D. K., Smolka A., Modlin I. M., Goldenring J. R. Characterization of membrane and cytoskeletal compartments in cultured parietal cells: immunofluorescence and confocal microscopic examination. Eur J Cell Biol. 1993 Feb;60(1):76–87. [PubMed] [Google Scholar]
  14. Tominaga K. Distribution of parietal cells in the antral mucosa of human stomachs. Gastroenterology. 1975 Dec;69(6):1201–1207. [PubMed] [Google Scholar]
  15. Wyatt J. I., Rathbone B. J., Sobala G. M., Shallcross T., Heatley R. V., Axon A. T., Dixon M. F. Gastric epithelium in the duodenum: its association with Helicobacter pylori and inflammation. J Clin Pathol. 1990 Dec;43(12):981–986. doi: 10.1136/jcp.43.12.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. el-Omar E., Penman I., Dorrian C. A., Ardill J. E., McColl K. E. Eradicating Helicobacter pylori infection lowers gastrin mediated acid secretion by two thirds in patients with duodenal ulcer. Gut. 1993 Aug;34(8):1060–1065. doi: 10.1136/gut.34.8.1060. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES