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. 1996 May;49(5):365–368. doi: 10.1136/jcp.49.5.365

Significance of endotoxin in lethal synergy between bacteria associated with sudden infant death syndrome: follow up study.

N M Sayers 1, D B Drucker 1, J A Morris 1, D R Telford 1
PMCID: PMC500472  PMID: 8707947

Abstract

AIM: To investigate the role of endotoxin in synergy between bacterial toxins associated with sudden infant death syndrome (SIDS). METHODS: Extracellular toxins of 13 isolates of Staphylococcus from SIDS victims and matched healthy infants were tested for lethal toxicity in chick embryos with and without standard endotoxin (used at 1.00 ng/embryo). Endotoxin and toxins from staphylococci were used at dilutions with negligible lethality. RESULTS: Simultaneous injection of non-lethal levels of endotoxin and toxins from 11 of the 13 staphylococcal isolates tested produced lethal toxicity that was 111 to 613% greater than expected by an additive effect alone. This was highly significant and occurred even in the absence of staphylococcal enterotoxins or toxic shock syndrome toxin-1. CONCLUSION: Endotoxin enhancement of staphylococcal toxin lethality could be partly responsible for the clinical outcome in SIDS.

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Selected References

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  1. Beezhold D. H., Best G. K., Bonventre P. F., Thompson M. Synergistic induction of interleukin-1 by endotoxin and toxic shock syndrome toxin-1 using rat macrophages. Infect Immun. 1987 Dec;55(12):2865–2869. doi: 10.1128/iai.55.12.2865-2869.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bentele K. H., Albani M. Are there tests predictive for prolonged apnoea and SIDS? A review of epidemiological and functional studies. Acta Paediatr Scand Suppl. 1988;342:1–21. doi: 10.1111/j.1651-2227.1988.tb10792.x. [DOI] [PubMed] [Google Scholar]
  3. Bettelheim K. A., Goldwater P. N., Dwyer B. W., Bourne A. J., Smith D. L. Toxigenic Escherichia coli associated with sudden infant death syndrome. Scand J Infect Dis. 1990;22(4):467–476. doi: 10.3109/00365549009027079. [DOI] [PubMed] [Google Scholar]
  4. Collie M. H., Rushton D. I., Sweet C., Smith H. Studies of influenza virus infection in newborn ferrets. J Med Microbiol. 1980 Nov;13(4):561–571. doi: 10.1099/00222615-13-4-561. [DOI] [PubMed] [Google Scholar]
  5. Corriveau C. C., Danner R. L. Antiendotoxin therapies for septic shock. Infect Agents Dis. 1993 Feb;2(1):44–52. [PubMed] [Google Scholar]
  6. Drucker D. B., Aluyi H. S., Morris J. A., Telford D. R., Gibbs A. Lethal synergistic action of toxins of bacteria isolated from sudden infant death syndrome. J Clin Pathol. 1992 Sep;45(9):799–801. doi: 10.1136/jcp.45.9.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eichhorn E. A. A TECHNIQUE FOR THE INTRAVENOUS INOCULATION OF CHICK EMBRYOS. Science. 1940 Sep 13;92(2385):245–246. doi: 10.1126/science.92.2385.245. [DOI] [PubMed] [Google Scholar]
  8. Holland I. B., Kenny B., Blight M. Haemolysin secretion from E coli. Biochimie. 1990 Feb-Mar;72(2-3):131–141. doi: 10.1016/0300-9084(90)90138-7. [DOI] [PubMed] [Google Scholar]
  9. Husseini R. H., Collie M. H., Rushton D. I., Sweet C., Smith H. The role of naturally-acquired bacterial infection in influenza-related death in neonatal ferrets. Br J Exp Pathol. 1983 Oct;64(5):559–569. [PMC free article] [PubMed] [Google Scholar]
  10. Jakeman K. J., Rushton D. I., Smith H., Sweet C. Exacerbation of bacterial toxicity to infant ferrets by influenza virus: possible role in sudden infant death syndrome. J Infect Dis. 1991 Jan;163(1):35–40. doi: 10.1093/infdis/163.1.35. [DOI] [PubMed] [Google Scholar]
  11. Li D. K., Daling J. R. Maternal smoking, low birth weight, and ethnicity in relation to sudden infant death syndrome. Am J Epidemiol. 1991 Nov 1;134(9):958–964. doi: 10.1093/oxfordjournals.aje.a116180. [DOI] [PubMed] [Google Scholar]
  12. Marrack P., Kappler J. The staphylococcal enterotoxins and their relatives. Science. 1990 May 11;248(4956):705–711. doi: 10.1126/science.2185544. [DOI] [PubMed] [Google Scholar]
  13. Martich G. D., Boujoukos A. J., Suffredini A. F. Response of man to endotoxin. Immunobiology. 1993 Apr;187(3-5):403–416. doi: 10.1016/S0171-2985(11)80353-0. [DOI] [PubMed] [Google Scholar]
  14. McKendrick N., Drucker D. B., Morris J. A., Telford D. R., Barson A. J., Oppenheim B. A., Crawley B. A., Gibbs A. Bacterial toxins: a possible cause of cot death. J Clin Pathol. 1992 Jan;45(1):49–53. doi: 10.1136/jcp.45.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Milner A. D. Recent theories on the cause of cot death. Br Med J (Clin Res Ed) 1987 Nov 28;295(6610):1366–1368. doi: 10.1136/bmj.295.6610.1366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morris J. A., Haran D., Smith A. Hypothesis: common bacterial toxins are a possible cause of the sudden infant death syndrome. Med Hypotheses. 1987 Feb;22(2):211–222. doi: 10.1016/0306-9877(87)90145-9. [DOI] [PubMed] [Google Scholar]
  17. Oppenheim B. A., Barclay G. R., Morris J., Knox F., Barson A., Drucker D. B., Crawley B. A., Morris J. A. Antibodies to endotoxin core in sudden infant death syndrome. Arch Dis Child. 1994 Feb;70(2):95–98. doi: 10.1136/adc.70.2.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SUGIYAMA H., McKISSIC E. M., Jr, BERGDOLL M. S. Sensitivity of thorotrast-treated monkeys to staphylococcal enterotoxin. Proc Soc Exp Biol Med. 1963 Jun;113:468–470. doi: 10.3181/00379727-113-28398. [DOI] [PubMed] [Google Scholar]
  19. Sayers N. M., Drucker D. B., Morris J. A., Telford D. R. Lethal synergy between toxins of staphylococci and enterobacteria: implications for sudden infant death syndrome. J Clin Pathol. 1995 Oct;48(10):929–932. doi: 10.1136/jcp.48.10.929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schlievert P. M., Bettin K. M., Watson D. W. Purification and characterization of group A streptococcal pyrogenic exotoxin type C. Infect Immun. 1977 May;16(2):673–679. doi: 10.1128/iai.16.2.673-679.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Spapen H. D., Diltoer M., Huyghens L. P. Passive immunotherapy of gram-negative bacteremia, sepsis and septic shock. Acta Clin Belg. 1993;48(1):20–29. doi: 10.1080/17843286.1993.11718281. [DOI] [PubMed] [Google Scholar]
  22. Telford D. R., Morris J. A., Hughes P., Conway A. R., Lee S., Barson A. J., Drucker D. B. The nasopharyngeal bacterial flora in the sudden infant death syndrome. J Infect. 1989 Mar;18(2):125–130. doi: 10.1016/s0163-4453(89)91094-3. [DOI] [PubMed] [Google Scholar]
  23. Wieneke A. A. The detection of enterotoxin and toxic shock syndrome toxin-1 production by strains of Staphylococcus aureus with commercial RPLA kits. Int J Food Microbiol. 1988 Aug;7(1):25–30. doi: 10.1016/0168-1605(88)90069-4. [DOI] [PubMed] [Google Scholar]
  24. Williams A. L., Uren E. C., Bretherton L. Respiratory viruses and sudden infant death. Br Med J (Clin Res Ed) 1984 May 19;288(6429):1491–1493. doi: 10.1136/bmj.288.6429.1491. [DOI] [PMC free article] [PubMed] [Google Scholar]

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