Table 2.
D10 purification. Protein assays and PTPRQ targeted proteomics assays were carried out on two preparations, each of ~1000 chick inner ears. Total PTPRQ PRM intensity was determined by multiplying the PTPRQ intensity from PRM targeted-proteomics experiments (per µg protein) by the total amount of protein (in µg) in each sample.
| Fraction | Description | Total protein (µg), ± range | PTPRQ PRM intensity, total | PTPRQ PRM intensity, fraction of PNS |
|---|---|---|---|---|
| S1 | Post nuclear supernatant | 109,000 ± 4,000 | 2.63 ± 0.40 × 1010 | 100 ± 15% |
| S2 | 7000 rpm supernatant | 100,000 ± 4,000 | 0.35 ± 0.05 | 13 ± 3% |
| P2 | Dense membranes | 36,400 ± 700 | 2.56 ± 0.17 | 97 ± 16% |
| M3 | D10 bound | 2680 ± 90 | 0.34 ± 0.04 | 13 ± 2% |
| S6 | Cytoplasm | 29,200 ± 100 | 0.32 ± 0.07 | 12 ± 2% |
| P6 | Membranes | 984 ± 1 | 0.28 ± 0.01 | 11 ± 2% |
| S7 | RIPA-soluble | 1037 ± 0 | 1.13 ± 0.11 | 43 ± 8% |