Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1996 Jun;49(6):474–477. doi: 10.1136/jcp.49.6.474

Expression of CD15 in normal and metaplastic Paneth cells of the digestive tract.

A Ariza 1, D López 1, E M Castellà 1, C Muñoz 1, M J Zújar 1, J L Mate 1
PMCID: PMC500537  PMID: 8763261

Abstract

AIMS: To substantiate that incubation with monoclonal antibody CD15 (C3D-1) elicits a distinctive immunoreaction in normal small intestinal Paneth cells, normal and metaplastic Paneth cells along the digestive tract were assessed to determine whether they are also immunoreactive to CD15. METHODS: Paneth cells in paraffin wax embedded specimens of normal small intestine, appendix and proximal ascending colon, and from cases of chronic gastritis and ulcerative colitis were investigated immunohistochemically for lysozyme and CD15 antigen expression by means of the avidin-biotin peroxidase complex method. RESULTS: CD15 antibody reacted with a high proportion of both normal and metaplastic Paneth cells. Paneth cell immunoreactivity to CD15, however, was less intense and less extensive than to antilysozyme antibody, though the latter also stained many other cell types and was more commonly associated with nonspecific background staining. CONCLUSIONS: CD15 seems to be a valuable adjuvant for the study of Paneth cells in the normal and diseased digestive tract. Furthermore, as CD15 has been shown to be involved in activation of phagocytes, its expression in Paneth cells reinforces their proposed role as antimicrobial agents and regulators of the intestinal flora.

Full text

PDF
474

Images in this article

475Image
on p.475

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adlakha H., Bostwick D. G. Paneth cell-like change in prostatic adenocarcinoma represents neuroendocrine differentiation: report of 30 cases. Hum Pathol. 1994 Feb;25(2):135–139. doi: 10.1016/0046-8177(94)90268-2. [DOI] [PubMed] [Google Scholar]
  2. Caruso R. A., La Spada F., Casablanca G., Rigoli L. Lysozyme and mucins in gastric adenomas. J Clin Pathol. 1989 Aug;42(8):827–833. doi: 10.1136/jcp.42.8.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Evans G. S., Chwalinski S., Owen G., Booth C., Singh A., Potten C. S. Expression of pokeweed lectin binding in murine intestinal Paneth cells. Epithelial Cell Biol. 1994 Jan;3(1):7–15. [PubMed] [Google Scholar]
  4. Ganz T., Lehrer R. I. Defensins. Curr Opin Immunol. 1994 Aug;6(4):584–589. doi: 10.1016/0952-7915(94)90145-7. [DOI] [PubMed] [Google Scholar]
  5. Geller S. A., Thung S. N. Morphologic unity of Paneth cells. Arch Pathol Lab Med. 1983 Sep;107(9):476–479. [PubMed] [Google Scholar]
  6. Gibbs N. M. Incidence and significance of argentaffin and paneth cells in some tumours of the large intestine. J Clin Pathol. 1967 Nov;20(6):826–831. doi: 10.1136/jcp.20.6.826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jones D. E., Bevins C. L. Defensin-6 mRNA in human Paneth cells: implications for antimicrobial peptides in host defense of the human bowel. FEBS Lett. 1993 Jan 4;315(2):187–192. doi: 10.1016/0014-5793(93)81160-2. [DOI] [PubMed] [Google Scholar]
  8. Kimura W., Futakawa N., Yamagata S., Wada Y., Kuroda A., Muto T., Esaki Y. Different clinicopathologic findings in two histologic types of carcinoma of papilla of Vater. Jpn J Cancer Res. 1994 Feb;85(2):161–166. doi: 10.1111/j.1349-7006.1994.tb02077.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lacasse J., Martin L. H. Detection of CD1 mRNA in Paneth cells of the mouse intestine by in situ hybridization. J Histochem Cytochem. 1992 Oct;40(10):1527–1534. doi: 10.1177/40.10.1382091. [DOI] [PubMed] [Google Scholar]
  10. Lee K. R., Trainer T. D. Adenocarcinoma of the uterine cervix of small intestinal type containing numerous Paneth cells. Arch Pathol Lab Med. 1990 Jul;114(7):731–733. [PubMed] [Google Scholar]
  11. Lev R., DeNucci T. D. Neoplastic Paneth cells in the stomach. Report of two cases and review of the literature. Arch Pathol Lab Med. 1989 Feb;113(2):129–133. [PubMed] [Google Scholar]
  12. Lund-Johansen F., Olweus J., Horejsi V., Skubitz K. M., Thompson J. S., Vilella R., Symington F. W. Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65). J Immunol. 1992 May 15;148(10):3221–3229. [PubMed] [Google Scholar]
  13. MORSON B. C. Intestinal metaplasia of the gastric mucosa. Br J Cancer. 1955 Sep;9(3):365–376. doi: 10.1038/bjc.1955.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mathan M., Hughes J., Whitehead R. The morphogenesis of the human Paneth cell. An immunocytochemical ultrastructural study. Histochemistry. 1987;87(1):91–96. doi: 10.1007/BF00518730. [DOI] [PubMed] [Google Scholar]
  15. Murata K., Egami H., Kiyohara H., Oshima S., Kurizaki T., Ogawa M. Expression of group-II phospholipase A2 in malignant and non-malignant human gastric mucosa. Br J Cancer. 1993 Jul;68(1):103–111. doi: 10.1038/bjc.1993.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nevalainen T. J., Grönroos J. M., Kallajoki M. Expression of group II phospholipase A2 in the human gastrointestinal tract. Lab Invest. 1995 Feb;72(2):201–208. [PubMed] [Google Scholar]
  17. Nistal M., Garcia-Rodeja E., Paniagua R. Granular transformation of Sertoli cells in testicular disorders. Hum Pathol. 1991 Feb;22(2):131–137. doi: 10.1016/0046-8177(91)90034-m. [DOI] [PubMed] [Google Scholar]
  18. Odze R., Gallinger S., So K., Antonioli D. Duodenal adenomas in familial adenomatous polyposis: relation of cell differentiation and mucin histochemical features to growth pattern. Mod Pathol. 1994 Apr;7(3):376–384. [PubMed] [Google Scholar]
  19. Ohtsuka A., Kikuta A., Taguchi T., Murakami T. A hydrophilic resin-embedding method for light and electron microscopic detection of tissue anionic sites with cationic colloidal iron: as applied to mouse Paneth cells. Arch Histol Cytol. 1993 Oct;56(4):423–430. doi: 10.1679/aohc.56.423. [DOI] [PubMed] [Google Scholar]
  20. Otis C. N., Carter D., Cole S., Battifora H. Immunohistochemical evaluation of pleural mesothelioma and pulmonary adenocarcinoma. A bi-institutional study of 47 cases. Am J Surg Pathol. 1987 Jun;11(6):445–456. doi: 10.1097/00000478-198706000-00005. [DOI] [PubMed] [Google Scholar]
  21. Sandow M. J., Whitehead R. The Paneth cell. Gut. 1979 May;20(5):420–431. doi: 10.1136/gut.20.5.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sawada M., Horiguchi Y., Abujiang P., Miyake N., Kitamura Y., Midorikawa O., Hiai H. Monoclonal antibodies to a zinc-binding protein of rat Paneth cells. J Histochem Cytochem. 1994 Apr;42(4):467–472. doi: 10.1177/42.4.8126373. [DOI] [PubMed] [Google Scholar]
  23. Sawada M., Horiguchi Y., Abujiang P., Miyake N., Kitamura Y., Midorikawa O., Hiai H. Monoclonal antibodies to a zinc-binding protein of rat Paneth cells. J Histochem Cytochem. 1994 Apr;42(4):467–472. doi: 10.1177/42.4.8126373. [DOI] [PubMed] [Google Scholar]
  24. Schienle H. W., Stein N., Müller-Ruchholtz W. Neutrophil granulocytic cell antigen defined by a monoclonal antibody--its distribution within normal haemic and non-haemic tissue. J Clin Pathol. 1982 Sep;35(9):959–966. doi: 10.1136/jcp.35.9.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Schmauder-Chock E. A., Chock S. P., Patchen M. L. Ultrastructural localization of tumour necrosis factor-alpha. Histochem J. 1994 Feb;26(2):142–151. doi: 10.1007/BF00157963. [DOI] [PubMed] [Google Scholar]
  26. Stein H., Gerdes J., Schwab U., Lemke H., Mason D. Y., Ziegler A., Schienle W., Diehl V. Identification of Hodgkin and Sternberg-reed cells as a unique cell type derived from a newly-detected small-cell population. Int J Cancer. 1982 Oct 15;30(4):445–459. doi: 10.1002/ijc.2910300411. [DOI] [PubMed] [Google Scholar]
  27. Stein H., Uchánska-Ziegler B., Gerdes J., Ziegler A., Wernet P. Hodgkin and Sternberg-Reed cells contain antigens specific to late cells of granulopoiesis. Int J Cancer. 1982 Mar 15;29(3):283–290. doi: 10.1002/ijc.2910290310. [DOI] [PubMed] [Google Scholar]
  28. Symonds D. A. Paneth cell metaplasia in diseases of the colon and rectum. Arch Pathol. 1974 Jun;97(6):343–347. [PubMed] [Google Scholar]
  29. Tan X., Hsueh W., Gonzalez-Crussi F. Cellular localization of tumor necrosis factor (TNF)-alpha transcripts in normal bowel and in necrotizing enterocolitis. TNF gene expression by Paneth cells, intestinal eosinophils, and macrophages. Am J Pathol. 1993 Jun;142(6):1858–1865. [PMC free article] [PubMed] [Google Scholar]
  30. Weaver M. G., Abdul-Karim F. W., Srigley J., Bostwick D. G., Ro J. Y., Ayala A. G. Paneth cell-like change of the prostate gland. A histological, immunohistochemical, and electron microscopic study. Am J Surg Pathol. 1992 Jan;16(1):62–68. doi: 10.1097/00000478-199201000-00009. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES