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. 1996 Aug;49(8):660–663. doi: 10.1136/jcp.49.8.660

Expression of IL-2R, IL-4R, IL-6R on peripheral blood lymphocytes in systemic lupus erythematosus and correlation with disease activity: a prospective study.

E Y Chan 1, C S Lau 1, H Zola 1
PMCID: PMC500611  PMID: 8881918

Abstract

AIMS: To study the expression of interleukin-2 receptor (IL-2R), interleukin-4 receptor (IL-4R) and interleukin-6 receptor (IL-6R) on peripheral blood lymphocytes (PBL) in patients with systemic lupus erythematosus (SLE); to correlate the level of expression of these receptors with SLE disease activity. METHODS: Peripheral blood lymphocytes were studied by a high sensitivity flow cytometry technique using monoclonal antibodies directed against CD25 (IL-2R alpha chain), CD122 (IL-2R beta chain), CD124 (IL-4R), and CD126 (IL-6R). SLE disease activity was scored using the SLE Disease Activity Index, C3 and C4 concentrations, anti-dsDNA level, and absolute lymphocyte count. RESULTS: Compared with normal controls, PBL from patients with SLE had a higher percentage of CD25+ cells (median 20.8% v 16.5%) and a lower percentage of CD122+ cells (median 13.1% v 22.4%). The difference in CD122+ cells was greater in the CD122weak population than the CD122strong (natural killer cell) population. The percentages of CD124+ and CD126+ PBLs in patients with SLE and controls were similar. On CD25+ cells, the relative antigenic level of the IL-2R alpha chain was significantly higher in patients with SLE (median 2.01 v 1.81). The relative antigenic levels of CD122+, CD124+ and CD126+ cells were similar in patients and controls. Neither the percentages nor the relative antigenic levels of all of these cytokine receptors were correlated with any of the parameters of disease activity. CONCLUSION: Lymphocyte activation in patients with SLE was evident from the increase in CD25 expression on PBL, with a reciprocal decrease in CD122 expression. As the expression of IL-2R, IL-4R, IL-6R did not correlate with disease activity, it seems that these cytokine/receptor systems do not play a direct role in disease activation in SLE.

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Selected References

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  1. Alcocer-Varela J., Alarcón-Riquelme M., Laffón A., Sánchez-Madrid F., Alarcón-Segovia D. Activation markers on peripheral blood T cells from patients with active or inactive systemic lupus erythematosus. Correlation with proliferative responses and production of IL-2. J Autoimmun. 1991 Dec;4(6):935–945. doi: 10.1016/0896-8411(91)90056-i. [DOI] [PubMed] [Google Scholar]
  2. Alcocer-Varela J., Alarcón-Segovia D. Decreased production of and response to interleukin-2 by cultured lymphocytes from patients with systemic lupus erythematosus. J Clin Invest. 1982 Jun;69(6):1388–1392. doi: 10.1172/JCI110579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Amasaki Y., Kobayashi S., Takeda T., Ogura N., Jodo S., Nakabayashi T., Tsutsumi A., Fujisaku A., Koike T. Up-regulated expression of Fas antigen (CD95) by peripheral naive and memory T cell subsets in patients with systemic lupus erythematosus (SLE): a possible mechanism for lymphopenia. Clin Exp Immunol. 1995 Feb;99(2):245–250. doi: 10.1111/j.1365-2249.1995.tb05540.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bombardier C., Gladman D. D., Urowitz M. B., Caron D., Chang C. H. Derivation of the SLEDAI. A disease activity index for lupus patients. The Committee on Prognosis Studies in SLE. Arthritis Rheum. 1992 Jun;35(6):630–640. doi: 10.1002/art.1780350606. [DOI] [PubMed] [Google Scholar]
  5. Cuadrado M. J., Marubayashi M., Ortega C., Fernandez-Arcas N., Garcia-Cozar F., Pena J., Sanchez-Guijo P., Santamaria M. Relationship of IL-2, IL-2R (CD25+), soluble IL-2R and IL-4 with disease activity in SLE patients. Lupus. 1993 Aug;2(4):257–260. doi: 10.1177/096120339300200113. [DOI] [PubMed] [Google Scholar]
  6. Emlen W., Niebur J., Kadera R. Accelerated in vitro apoptosis of lymphocytes from patients with systemic lupus erythematosus. J Immunol. 1994 Apr 1;152(7):3685–3692. [PubMed] [Google Scholar]
  7. Huang Y. P., Perrin L. H., Miescher P. A., Zubler R. H. Correlation of T and B cell activities in vitro and serum IL-2 levels in systemic lupus erythematosus. J Immunol. 1988 Aug 1;141(3):827–833. [PubMed] [Google Scholar]
  8. Ishida H., Kumagai S., Umehara H., Sano H., Tagaya Y., Yodoi J., Imura H. Impaired expression of high affinity interleukin 2 receptor on activated lymphocytes from patients with systemic lupus erythematosus. J Immunol. 1987 Aug 15;139(4):1070–1074. [PubMed] [Google Scholar]
  9. Ishii N., Takeshita T., Kimura Y., Tada K., Kondo M., Nakamura M., Sugamura K. Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood. Int Immunol. 1994 Aug;6(8):1273–1277. doi: 10.1093/intimm/6.8.1273. [DOI] [PubMed] [Google Scholar]
  10. Janossy G., Borthwick N., Lomnitzer R., Medina E., Squire S. B., Phillips A. N., Lipman M., Johnson M. A., Lee C., Bofill M. Lymphocyte activation in HIV-1 infection. I. Predominant proliferative defects among CD45R0+ cells of the CD4 and CD8 lineages. AIDS. 1993 May;7(5):613–624. doi: 10.1097/00002030-199305000-00002. [DOI] [PubMed] [Google Scholar]
  11. Linker-Israeli M., Deans R. J., Wallace D. J., Prehn J., Ozeri-Chen T., Klinenberg J. R. Elevated levels of endogenous IL-6 in systemic lupus erythematosus. A putative role in pathogenesis. J Immunol. 1991 Jul 1;147(1):117–123. [PubMed] [Google Scholar]
  12. Nagafuchi H., Suzuki N., Mizushima Y., Sakane T. Constitutive expression of IL-6 receptors and their role in the excessive B cell function in patients with systemic lupus erythematosus. J Immunol. 1993 Dec 1;151(11):6525–6534. [PubMed] [Google Scholar]
  13. Raziuddin S., al-Janadi M. A., al-Wabel A. A. Soluble interleukin 2 receptor levels in serum and its relationship to T cell abnormality and clinical manifestations of the disease in patients with systemic lupus erythematosus. J Rheumatol. 1991 Jun;18(6):831–836. [PubMed] [Google Scholar]
  14. Sheldon A., Flego L., Zola H. Coexpression of IL-2 receptor p55 and p75 by circulating blood lymphocytes. J Leukoc Biol. 1993 Aug;54(2):161–167. doi: 10.1002/jlb.54.2.161. [DOI] [PubMed] [Google Scholar]
  15. Spronk P. E., vd Gun B. T., Limburg P. C., Kallenberg C. G. B cell activation in clinically quiescent systemic lupus erythematosus (SLE) is related to immunoglobulin levels, but not to levels of anti-dsDNA, nor to concurrent T cell activation. Clin Exp Immunol. 1993 Jul;93(1):39–44. doi: 10.1111/j.1365-2249.1993.tb06494.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]
  17. Tanaka T., Saiki O., Negoro S., Igarashi T., Kuritani T., Hara H., Suemura M., Kishimoto S. Decreased expression of interleukin-2 binding molecules (p70/75) in T cells from patients with systemic lupus erythematosus. Arthritis Rheum. 1989 May;32(5):552–559. doi: 10.1002/anr.1780320507. [DOI] [PubMed] [Google Scholar]
  18. Via C. S., Handwerger B. S. B-cell and T-cell function in systemic lupus erythematosus. Curr Opin Rheumatol. 1993 Sep;5(5):570–574. doi: 10.1097/00002281-199305050-00004. [DOI] [PubMed] [Google Scholar]
  19. Wigfall D. R., Sakai R. S., Wallace D. J., Jordan S. C. Interleukin-2 receptor expression in peripheral blood lymphocytes from systemic lupus erythematosus patients: relationship to clinical activity. Clin Immunol Immunopathol. 1988 Jun;47(3):354–362. doi: 10.1016/s0090-1229(88)80012-6. [DOI] [PubMed] [Google Scholar]
  20. Zola H., Flego L., Sheldon A. Detection of cytokine receptors by high-sensitivity immunofluorescence/flow cytometry. Immunobiology. 1992 Aug;185(2-4):350–365. doi: 10.1016/S0171-2985(11)80652-2. [DOI] [PubMed] [Google Scholar]
  21. al-Janadi M., Raziuddin S. B cell hyperactivity is a function of T cell derived cytokines in systemic lupus erythematosus. J Rheumatol. 1993 Nov;20(11):1885–1891. [PubMed] [Google Scholar]
  22. ter Borg E. J., Horst G., Limburg P. C., Kallenberg C. G. Changes in plasma levels of interleukin-2 receptor in relation to disease exacerbations and levels of anti-dsDNA and complement in systemic lupus erythematosus. Clin Exp Immunol. 1990 Oct;82(1):21–26. doi: 10.1111/j.1365-2249.1990.tb05398.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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