Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1996 Aug;49(8):686–687. doi: 10.1136/jcp.49.8.686

Isolation of Mycoplasma hominis using the BACTEC 9000 series blood culture system.

K A Kitson 1, K C Wright 1
PMCID: PMC500619  PMID: 8881926

Abstract

Mycoplasma hominis has been implicated as an important cause of septicaemia. There have been reported variances in the ability of blood culture systems to support the growth of this organism. In this study the ability of the BACTEC 9000 series automated system to grow and detect M hominis was assessed. Three of five wild M hominis strains grew in the BACTEC Anaerobic Plus/F medium but growth was not flagged by the detection mechanism of the system. It is recommended that users of the BACTEC 9000 series should use a seven day protocol and perform terminal subculture for suspected cases of M hominis septicaemia.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davies S., Eggington R. Recovery of Mycoplasma hominis from blood culture media. Med Lab Sci. 1991 Apr;48(2):110–113. [PubMed] [Google Scholar]
  2. Davies S., Spencer R. C. Effect of blood culture media on the in vitro recovery of Mycoplasma hominis. J Infect. 1988 Nov;17(3):215–220. doi: 10.1016/s0163-4453(88)96474-2. [DOI] [PubMed] [Google Scholar]
  3. Kelly V. N., Garland S. M., Gilbert G. L. Isolation of genital mycoplasmas from the blood of neonates and women with pelvic infection using conventional SPS-free blood culture media. Pathology. 1987 Jul;19(3):277–280. doi: 10.3109/00313028709066563. [DOI] [PubMed] [Google Scholar]
  4. McMahon D. K., Dummer J. S., Pasculle A. W., Cassell G. Extragenital Mycoplasma hominis infections in adults. Am J Med. 1990 Sep;89(3):275–281. doi: 10.1016/0002-9343(90)90338-e. [DOI] [PubMed] [Google Scholar]
  5. Smaron M. F., Boonlayangoor S., Zierdt C. H. Detection of Mycoplasma hominis septicemia by radiometric blood culture. J Clin Microbiol. 1985 Mar;21(3):298–301. doi: 10.1128/jcm.21.3.298-301.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. al-Zahawi M. F., Kearns A. M., Sprott M. S., Joudeh M., Snodgrass C. A. A study of three blood culture media for isolating genital mycoplasmas from obstetrical and gynaecological patients. J Infect. 1990 Sep;21(2):143–150. doi: 10.1016/0163-4453(90)91623-l. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES