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. 2015 Dec 8;37(2):119–128. doi: 10.1093/carcin/bgv169

Figure 3.

Figure 3.

JMJD6 increases self-renewal capacity and anchorage independent growth of SCC4. (A) JMJD6 expression was forced in SCC4 by infecting with retroviral vector expressing Flag-JMJD6, and its ectopic expression was confirmed by Western blot. (B) Effect of JMJD6 overexpression on self-renewal capacity of SCC4 was determined by tumor sphere formation assay. Data are means ± SD of triplicate experiments. *P < 0.05, unpaired two-tailed Student’s t test. (C) Representative image of tumor spheres formed by SCC4 infected with control empty virus (SCC4/EV) and virus expressing Flag-JMJD6 (SCC4/JMJD6). (D) Effect of JMJD6 overexpression on anchorage-independent growth of SCC4 was determined by soft agar assay. **P < 0.01, unpaired two-tailed Student’s t test.