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. 1998 Jun;51(6):444–449. doi: 10.1136/jcp.51.6.444

In situ localisation of Yersinia enterocolitica by catalysed reported deposition signal amplification.

P T Odinot 1, J F Meis 1, J A Hoogkamp-Korstanje 1, W J Melchers 1
PMCID: PMC500747  PMID: 9771443

Abstract

AIM: The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH). METHODS: Y enterocolitica infected cell lines, rat spleens, and patient biopsy specimens were used to compare conventional ISH, immune fluorescence assay (IFA) detection, and catalysed reporter deposition (CARD) signal amplification ISH. RESULTS: CARD-ISH was shown to be more sensitive then conventional ISH and had a comparable sensitivity to IFA. In contrast to IFA, CARD-ISH preserved good tissue morphology. CONCLUSIONS: CARD-ISH appeared to be a fast and sensitive ISH method for detecting Y enterocolitica in routinely processed tissue sections. Application of this method allows the combination of routine detection and cellular localisation of the pathogen within the infected tissue.

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Selected References

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