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. 1996 Nov;49(11):892–896. doi: 10.1136/jcp.49.11.892

Oncogenic human papillomaviruses and ploidy in cervical lesions.

S Rihet 1, M Lorenzato 1, C Clavel 1
PMCID: PMC500827  PMID: 8944607

Abstract

AIM: To compare ploidy measurements obtained on tissue sections of selected low and high grade squamous intraepithelial lesions containing oncogenic HPV (types 16, 18 or 33) detected by in situ hybridisation (ISH) or PCR. METHODS: DNA ploidy was assessed by image cytometry after Feulgen staining of contiguous serial sections of eight lesions exhibiting atypical squamous cells or squamous atypia and 53 low and 63 high grade squamous intraepithelial lesions in which HPV had been detected by ISH or PCR. RESULTS: Aneuploidy was strongly associated with the presence of oncogenic HPV, being detected in 50% of lesions with squamous atypia and 75.5% of the low and 95.2% of the high grade squamous intraepithelial lesions. The multiploid profile was highly associated with high grade lesions and with the pattern of HPV DNA integration. CONCLUSIONS: The presence of aneuploidy is strongly suggestive of the presence of oncogenic HPV types. Combining the detection of HPV by ISH and PCR with DNA image cytometry may provide the pathologist and the physician with important prognostic information about low grade lesions, especially when these lesions have a multiploid DNA profile and contain oncogenic HPV.

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Selected References

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  1. Bibbo M., Dytch H. E., Alenghat E., Bartels P. H., Wied G. L. DNA ploidy profiles as prognostic indicators in CIN lesions. Am J Clin Pathol. 1989 Sep;92(3):261–265. doi: 10.1093/ajcp/92.3.261. [DOI] [PubMed] [Google Scholar]
  2. Binninger I., Clavel C., Polette M., Boutterin M. C., Chypre C., Alpha B., Birembaut P. A new nonisotopic detection of human papillomavirus DNA using polymerase chain reaction. Diagn Mol Pathol. 1992 Dec;1(4):239–245. [PubMed] [Google Scholar]
  3. Bosch F. X., Manos M. M., Muñoz N., Sherman M., Jansen A. M., Peto J., Schiffman M. H., Moreno V., Kurman R., Shah K. V. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group. J Natl Cancer Inst. 1995 Jun 7;87(11):796–802. doi: 10.1093/jnci/87.11.796. [DOI] [PubMed] [Google Scholar]
  4. Chacho M. S., Eppich E., Wersto R., Koss L. G. Influence of human papillomavirus on DNA ploidy determination in genital condylomas. Cancer. 1990 May 15;65(10):2291–2294. doi: 10.1002/1097-0142(19900515)65:10<2291::aid-cncr2820651021>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  5. Clavel C., Binninger I., Boutterin M. C., Polette M., Birembaut P. Comparison of four non-radioactive and 35S-based methods for the detection of human papillomavirus DNA by in situ hybridization. J Virol Methods. 1991 Aug;33(3):253–266. doi: 10.1016/0166-0934(91)90025-u. [DOI] [PubMed] [Google Scholar]
  6. Clavel C., Zerat L., Binninger I., Boutterin M. C., Polette M., Monsonego J., Birembaut P. DNA content measurement and in situ hybridization in condylomatous cervical lesions. Diagn Mol Pathol. 1992 Sep;1(3):180–184. [PubMed] [Google Scholar]
  7. Cooper K., Herrington C. S., Stickland J. E., Evans M. F., McGee J. O. Episomal and integrated human papillomavirus in cervical neoplasia shown by non-isotopic in situ hybridisation. J Clin Pathol. 1991 Dec;44(12):990–996. doi: 10.1136/jcp.44.12.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cox J. T., Lorincz A. T., Schiffman M. H., Sherman M. E., Cullen A., Kurman R. J. Human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance. Am J Obstet Gynecol. 1995 Mar;172(3):946–954. doi: 10.1016/0002-9378(95)90026-8. [DOI] [PubMed] [Google Scholar]
  9. Cuzick J., Terry G., Ho L., Hollingworth T., Anderson M. Human papillomavirus type 16 in cervical smears as predictor of high-grade cervical intraepithelial neoplasia [corrected]. Lancet. 1992 Apr 18;339(8799):959–960. doi: 10.1016/0140-6736(92)91532-d. [DOI] [PubMed] [Google Scholar]
  10. De Vita R., Calugi A., Maggi F., Mauro F., Montevecchi L., Vecchione A. Flow cytometric DNA analysis of the human cervix affected by human papillomavirus and/or intraepithelial neoplasia. Anal Quant Cytol Histol. 1990 Oct;12(5):306–313. [PubMed] [Google Scholar]
  11. Del Mistro A., Koss L. G., Braunstein J., Bennett B., Saccomano G., Simons K. M. Condylomata acuminata of the urinary bladder. Natural history, viral typing, and DNA content. Am J Surg Pathol. 1988 Mar;12(3):205–215. doi: 10.1097/00000478-198803000-00006. [DOI] [PubMed] [Google Scholar]
  12. Downey G. P., Bavin P. J., Deery A. R., Crow J., Griffiths P. D., Emery V. C., Walker P. G. Relation between human papillomavirus type 16 and potential for progression of minor-grade cervical disease. Lancet. 1994 Aug 13;344(8920):432–435. doi: 10.1016/s0140-6736(94)91768-x. [DOI] [PubMed] [Google Scholar]
  13. Dudzinski M. R., Haskill S. J., Fowler W. C., Currie J. L., Walton L. A. DNA content in cervical neoplasia and its relationship to prognosis. Obstet Gynecol. 1987 Mar;69(3 Pt 1):373–377. [PubMed] [Google Scholar]
  14. Evans A. S., Monaghan J. M. Nuclear DNA content of normal, neoplastic and "wart-affected" cervical biopsies. Anal Quant Cytol. 1983 Jun;5(2):112–116. [PubMed] [Google Scholar]
  15. Fu Y. S., Braun L., Shah K. V., Lawrence W. D., Robboy S. J. Histologic, nuclear DNA, and human papillomavirus studies of cervical condylomas. Cancer. 1983 Nov 1;52(9):1705–1711. doi: 10.1002/1097-0142(19831101)52:9<1705::aid-cncr2820520926>3.0.co;2-d. [DOI] [PubMed] [Google Scholar]
  16. Fu Y. S., Reagan J. W., Richart R. M. Definition of precursors. Gynecol Oncol. 1981 Oct;12(2 Pt 2):S220–S231. doi: 10.1016/0090-8258(81)90076-7. [DOI] [PubMed] [Google Scholar]
  17. Fujii T., Crum C. P., Winkler B., Fu Y. S., Richart R. M. Human papillomavirus infection and cervical intraepithelial neoplasia: histopathology and DNA content. Obstet Gynecol. 1984 Jan;63(1):99–104. [PubMed] [Google Scholar]
  18. Gilles C., Piette J., Rombouts S., Laurent C., Foidart J. M. Immortalization of human cervical keratinocytes by human papillomavirus type 33. Int J Cancer. 1993 Mar 12;53(5):872–879. doi: 10.1002/ijc.2910530527. [DOI] [PubMed] [Google Scholar]
  19. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  20. Herman C. J. Cytometric DNA analysis in the management of cancer. Clinical and laboratory considerations. Cancer. 1992 Mar 15;69(6 Suppl):1553–1556. doi: 10.1002/1097-0142(19920315)69:6+<1553::aid-cncr2820691308>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  21. Hurlin P. J., Kaur P., Smith P. P., Perez-Reyes N., Blanton R. A., McDougall J. K. Progression of human papillomavirus type 18-immortalized human keratinocytes to a malignant phenotype. Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):570–574. doi: 10.1073/pnas.88.2.570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Koutsky L. A., Holmes K. K., Critchlow C. W., Stevens C. E., Paavonen J., Beckmann A. M., DeRouen T. A., Galloway D. A., Vernon D., Kiviat N. B. A cohort study of the risk of cervical intraepithelial neoplasia grade 2 or 3 in relation to papillomavirus infection. N Engl J Med. 1992 Oct 29;327(18):1272–1278. doi: 10.1056/NEJM199210293271804. [DOI] [PubMed] [Google Scholar]
  23. Lorenzato M., Lallemand A., Visseaux-Coletto B., Gaillard D. Quantification de l'ADN par analyse d'image sur coupes en paraffine. Ann Pathol. 1994;14(4):257–260. [PubMed] [Google Scholar]
  24. Lorincz A. T., Reid R., Jenson A. B., Greenberg M. D., Lancaster W., Kurman R. J. Human papillomavirus infection of the cervix: relative risk associations of 15 common anogenital types. Obstet Gynecol. 1992 Mar;79(3):328–337. doi: 10.1097/00006250-199203000-00002. [DOI] [PubMed] [Google Scholar]
  25. Pei X. F., Gorman P. A., Watt F. M. Two strains of human keratinocytes transfected with HPV16 DNA: comparison with the normal parental cells. Carcinogenesis. 1991 Feb;12(2):277–284. doi: 10.1093/carcin/12.2.277. [DOI] [PubMed] [Google Scholar]
  26. Remmink A. J., Walboomers J. M., Helmerhorst T. J., Voorhorst F. J., Rozendaal L., Risse E. K., Meijer C. J., Kenemans P. The presence of persistent high-risk HPV genotypes in dysplastic cervical lesions is associated with progressive disease: natural history up to 36 months. Int J Cancer. 1995 May 4;61(3):306–311. doi: 10.1002/ijc.2910610305. [DOI] [PubMed] [Google Scholar]
  27. Shevchuk M. M., Richart R. M. DNA content of condyloma acuminatum. Cancer. 1982 Feb 1;49(3):489–492. doi: 10.1002/1097-0142(19820201)49:3<489::aid-cncr2820490316>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  28. Syrjänen K. J. Epidemiology of human papillomavirus (HPV) infections and their associations with genital squamous cell cancer. Review article. APMIS. 1989 Nov;97(11):957–970. doi: 10.1111/j.1699-0463.1989.tb00504.x. [DOI] [PubMed] [Google Scholar]
  29. zur Hausen H. Human papillomaviruses in the pathogenesis of anogenital cancer. Virology. 1991 Sep;184(1):9–13. doi: 10.1016/0042-6822(91)90816-t. [DOI] [PubMed] [Google Scholar]

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