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. 1996 Nov;49(11):938–941. doi: 10.1136/jcp.49.11.938

HTLV Tax gene expression in patients with lymphoproliferative disorders.

E A Cardoso 1, N Miranda 1, P Gameiro 1, M J Frade 1, M Figueiredo 1, A Parreira 1
PMCID: PMC500836  PMID: 8944616

Abstract

AIMS: To study the expression of the human T lymphotropic virus (HTLV) Tax gene in peripheral blood mononuclear cells. METHODS: Blood was collected from 72 patients with lymphoproliferative disorders. Serum from all patients was assayed for antibodies directed against HTLV-I structural proteins by ELISA and western blotting. RNA was purified from fresh blood cells and amplified by reverse transcription polymerase chain reaction (RT-PCR). After Southern blotting, the PCR products were hybridised with a 32P end-labelled probe specific for the Tax gene. RESULTS: All samples were seronegative. A specific band for the Tax gene was found in five samples. Each of the patients positive for Tax gene expression had a different type of lymphoproliferative disorder. CONCLUSIONS: Infection by HTLV-I cannot be assessed solely by immunological assays, particularly when only disrupted virions are used. Sensitive molecular biology assays are essential for detecting viral gene expression in fresh blood cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banki K., Maceda J., Hurley E., Ablonczy E., Mattson D. H., Szegedy L., Hung C., Perl A. Human T-cell lymphotropic virus (HTLV)-related endogenous sequence, HRES-1, encodes a 28-kDa protein: a possible autoantigen for HTLV-I gag-reactive autoantibodies. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1939–1943. doi: 10.1073/pnas.89.5.1939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blattner W. A., Gibbs W. N., Saxinger C., Robert-Guroff M., Clark J., Lofters W., Hanchard B., Campbell M., Gallo R. C. Human T-cell leukaemia/lymphoma virus-associated lymphoreticular neoplasia in Jamaica. Lancet. 1983 Jul 9;2(8341):61–64. doi: 10.1016/s0140-6736(83)90056-9. [DOI] [PubMed] [Google Scholar]
  3. Cardoso E. A., Robert-Guroff M., Franchini G., Gartner S., Moura-Nunes J. F., Gallo R. C., Terrinha A. M. Seroprevalence of HTLV-I in Portugal and evidence of double retrovirus infection of a healthy donor. Int J Cancer. 1989 Feb 15;43(2):195–200. doi: 10.1002/ijc.2910430204. [DOI] [PubMed] [Google Scholar]
  4. Dalgleish A., Richardson J., Matutes E., Cruickshank K., Newell A., Sinclair A., Thorpe R., Brasher M., Weber J., Catovsky D. HTLV-1 infection in tropical spastic paraparesis: lymphocyte culture and serologic response. AIDS Res Hum Retroviruses. 1988 Dec;4(6):475–485. doi: 10.1089/aid.1988.4.475. [DOI] [PubMed] [Google Scholar]
  5. Dittmer J., Gitlin S. D., Reid R. L., Brady J. N. Transactivation of the P2 promoter of parathyroid hormone-related protein by human T-cell lymphotropic virus type I Tax1: evidence for the involvement of transcription factor Ets1. J Virol. 1993 Oct;67(10):6087–6095. doi: 10.1128/jvi.67.10.6087-6095.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duggan D. B., Ehrlich G. D., Davey F. P., Kwok S., Sninsky J., Goldberg J., Baltrucki L., Poiesz B. J. HTLV-I-induced lymphoma mimicking Hodgkin's disease. Diagnosis by polymerase chain reaction amplification of specific HTLV-I sequences in tumor DNA. Blood. 1988 Apr;71(4):1027–1032. [PubMed] [Google Scholar]
  7. Ehrlich G. D., Glaser J. B., Abbott M. A., Slamon D. J., Keith D., Sliwkowski M., Brandis J., Keitelman E., Teramoto Y., Papsidero L. Detection of anti-HTLV-I Tax antibodies in HTLV-I enzyme-linked immunosorbent assay-negative individuals. Blood. 1989 Aug 15;74(3):1066–1072. [PubMed] [Google Scholar]
  8. Ensoli B., Barillari G., Salahuddin S. Z., Gallo R. C., Wong-Staal F. Tat protein of HIV-1 stimulates growth of cells derived from Kaposi's sarcoma lesions of AIDS patients. Nature. 1990 May 3;345(6270):84–86. doi: 10.1038/345084a0. [DOI] [PubMed] [Google Scholar]
  9. Franchini G., Mann D. L., Popovic M., Zicht R. R., Gallo R. C., Wong-Staal F. HTLV-I infection of T and B cells of a patient with adult T-cell leukemia-lymphoma (ATLL) and transmission of HTLV-I from B cells to normal T cells. Leuk Res. 1985;9(11):1305–1314. doi: 10.1016/0145-2126(85)90116-x. [DOI] [PubMed] [Google Scholar]
  10. Franchini G., Wong-Staal F., Gallo R. C. Human T-cell leukemia virus (HTLV-I) transcripts in fresh and cultured cells of patients with adult T-cell leukemia. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6207–6211. doi: 10.1073/pnas.81.19.6207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fujii M., Tsuchiya H., Chuhjo T., Minamino T., Miyamoto K., Seiki M. Serum response factor has functional roles both in indirect binding to the CArG box and in the transcriptional activation function of human T-cell leukemia virus type I Tax. J Virol. 1994 Nov;68(11):7275–7283. doi: 10.1128/jvi.68.11.7275-7283.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Garin B., Gosselin S., de Thé G., Gessain A. HTLV-I/II infection in a high viral endemic area of Zaire, Central Africa: comparative evaluation of serology, PCR, and significance of indeterminate western blot pattern. J Med Virol. 1994 Sep;44(1):104–109. doi: 10.1002/jmv.1890440119. [DOI] [PubMed] [Google Scholar]
  13. Gessain A., Barin F., Vernant J. C., Gout O., Maurs L., Calender A., de Thé G. Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis. Lancet. 1985 Aug 24;2(8452):407–410. doi: 10.1016/s0140-6736(85)92734-5. [DOI] [PubMed] [Google Scholar]
  14. Gitlin S. D., Dittmer J., Shin R. C., Brady J. N. Transcriptional activation of the human T-lymphotropic virus type I long terminal repeat by functional interaction of Tax1 and Ets1. J Virol. 1993 Dec;67(12):7307–7316. doi: 10.1128/jvi.67.12.7307-7316.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Grassmann R., Dengler C., Müller-Fleckenstein I., Fleckenstein B., McGuire K., Dokhelar M. C., Sodroski J. G., Haseltine W. A. Transformation to continuous growth of primary human T lymphocytes by human T-cell leukemia virus type I X-region genes transduced by a Herpesvirus saimiri vector. Proc Natl Acad Sci U S A. 1989 May;86(9):3351–3355. doi: 10.1073/pnas.86.9.3351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Inoue J., Seiki M., Taniguchi T., Tsuru S., Yoshida M. Induction of interleukin 2 receptor gene expression by p40x encoded by human T-cell leukemia virus type 1. EMBO J. 1986 Nov;5(11):2883–2888. doi: 10.1002/j.1460-2075.1986.tb04583.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kalyanaraman V. S., Sarngadharan M. G., Robert-Guroff M., Miyoshi I., Golde D., Gallo R. C. A new subtype of human T-cell leukemia virus (HTLV-II) associated with a T-cell variant of hairy cell leukemia. Science. 1982 Nov 5;218(4572):571–573. doi: 10.1126/science.6981847. [DOI] [PubMed] [Google Scholar]
  18. Koyanagi Y., Itoyama Y., Nakamura N., Takamatsu K., Kira J., Iwamasa T., Goto I., Yamamoto N. In vivo infection of human T-cell leukemia virus type I in non-T cells. Virology. 1993 Sep;196(1):25–33. doi: 10.1006/viro.1993.1451. [DOI] [PubMed] [Google Scholar]
  19. LaGrenade L., Hanchard B., Fletcher V., Cranston B., Blattner W. Infective dermatitis of Jamaican children: a marker for HTLV-I infection. Lancet. 1990 Dec 1;336(8727):1345–1347. doi: 10.1016/0140-6736(90)92896-p. [DOI] [PubMed] [Google Scholar]
  20. Longo D. L., Gelmann E. P., Cossman J., Young R. A., Gallo R. C., O'Brien S. J., Matis L. A. Isolation of HTLV-transformed B-lymphocyte clone from a patient with HTLV-associated adult T-cell leukaemia. Nature. 1984 Aug 9;310(5977):505–506. doi: 10.1038/310505a0. [DOI] [PubMed] [Google Scholar]
  21. Mann D. L., DeSantis P., Mark G., Pfeifer A., Newman M., Gibbs N., Popovic M., Sarngadharan M. G., Gallo R. C., Clark J. HTLV-I--associated B-cell CLL: indirect role for retrovirus in leukemogenesis. Science. 1987 May 29;236(4805):1103–1106. doi: 10.1126/science.2883731. [DOI] [PubMed] [Google Scholar]
  22. Nagy K., Clapham P., Cheingsong-Popov R., Weiss R. A. Human T-cell leukemia virus type I: induction of syncytia and inhibition by patients' sera. Int J Cancer. 1983 Sep 15;32(3):321–328. doi: 10.1002/ijc.2910320310. [DOI] [PubMed] [Google Scholar]
  23. Pfisterer M., Burkart F., Jockers G., Meyer B., Regenass S., Burckhardt D., Schmitt H. E., Müller-Brand J., Skarvan K., Stulz P. Trial of low-dose aspirin plus dipyridamole versus anticoagulants for prevention of aortocoronary vein graft occlusion. Lancet. 1989 Jul 1;2(8653):1–7. doi: 10.1016/s0140-6736(89)90253-5. [DOI] [PubMed] [Google Scholar]
  24. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. doi: 10.1073/pnas.77.12.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Setoyama M., Fujiyoshi T., Mizoguchi S., Katahira Y., Yashiki S., Tara M., Kanzaki T., Sonoda S. HTLV-I messenger RNA is expressed in vivo in adult T-cell leukemia/lymphoma patients: an in situ hybridization study. Int J Cancer. 1994 Jun 1;57(5):760–764. doi: 10.1002/ijc.2910570525. [DOI] [PubMed] [Google Scholar]
  26. Shaw G. M., Gonda M. A., Flickinger G. H., Hahn B. H., Gallo R. C., Wong-Staal F. Genomes of evolutionarily divergent members of the human T-cell leukemia virus family (HTLV-I and HTLV-II) are highly conserved, especially in pX. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4544–4548. doi: 10.1073/pnas.81.14.4544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sugimoto M., Nakashima H., Watanabe S., Uyama E., Tanaka F., Ando M., Araki S., Kawasaki S. T-lymphocyte alveolitis in HTLV-I-associated myelopathy. Lancet. 1987 Nov 21;2(8569):1220–1220. doi: 10.1016/s0140-6736(87)91362-6. [DOI] [PubMed] [Google Scholar]
  28. Suzuki T., Hirai H., Murakami T., Yoshida M. Tax protein of HTLV-1 destabilizes the complexes of NF-kappa B and I kappa B-alpha and induces nuclear translocation of NF-kappa B for transcriptional activation. Oncogene. 1995 Mar 16;10(6):1199–1207. [PubMed] [Google Scholar]
  29. Vernant J. C., Buisson G., Magdeleine J., De Thore J., Jouannelle A., Neisson-Vernant C., Monplaisir N. T-lymphocyte alveolitis, tropical spastic paresis, and Sjögren syndrome. Lancet. 1988 Jan 23;1(8578):177–177. doi: 10.1016/s0140-6736(88)92744-4. [DOI] [PubMed] [Google Scholar]
  30. Wano Y., Feinberg M., Hosking J. B., Bogerd H., Greene W. C. Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9733–9737. doi: 10.1073/pnas.85.24.9733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yamaguchi K., Mochizuki M., Watanabe T., Miyata N., Tajima K., Mori S., Takatsuki K. Human T-lymphotropic virus type I uveitis. Leukemia. 1994 Apr;8 (Suppl 1):S88–S90. [PubMed] [Google Scholar]
  32. Yoshida M., Miyoshi I., Hinuma Y. Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2031–2035. doi: 10.1073/pnas.79.6.2031. [DOI] [PMC free article] [PubMed] [Google Scholar]

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