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(A–F) ZO-1 immunofluorescence staining of 3D-RPE and SD-RPE cells at day 40, day 60 and day 100 after differentiation. (G) TER measurements of 3D-RPE and SD-RPE cells (bars: mean + s.d.; n = 4). (H–M) The location of Na, K-ATPase in 3D-RPE and SD-RPE cells at day 40, day 60 and day 100 after differentiation.
