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. 2016 Mar 18;7(16):22819–22833. doi: 10.18632/oncotarget.8185

Figure 8. 3D-RPE cells rescued retinal degeneration when grafted into the subretinal space of RCS rats.

Figure 8

(A) Immunofluorescence staining showed colocalisation of human mitochondria and RPE65 in Dil-labeled transplanted 3D-RPE cells. Arrows indicated viable transplanted 3D-RPE cells which were human mitochondria, RPE65 and Dil positive. (B) Immunofluorescence staining showed the absence of Ki67 in transplanted 3D-RPE cells. Arrows indicated viable transplanted 3D-RPE cells with human mitochondria and Dil positive. (C) Representative immunofluorescence images of Rhodopsin in central transplanted area of 3D-RPE cells and SD-RPE cells and opposite transplanted area. (D) Thickened ONL (ONL thickness of central transplanted area subtracted ONL thickness in opposite transplanted area) of 3D-RPE and SD-RPE cells (bars: mean + s.d.). (E) Representative ERG traces at 4, 8 and 12 weeks after transplantation. (F) The mean B-wave amplitudes at 0 db showed similar visual function rescue after 3D-RPE and SD-RPE cells transplantation (bars: mean + s.d.). Single asterisk (*) indicated p value < 0.05. Double asterisk (**) indicated p value < 0.01. Abbreviations: outer nuclear layer (ONL); inner nuclear layer (INL); ganglion cell layer (GCL).