Abstract
AIM: To investigate whether human herpesvirus 6 (HHV-6) can cause latent infection of liver tissue. METHODS: Peripheral blood and liver tissue were collected from 25 living related liver transplant recipients at the time of transplantation. An avidin-biotin complex peroxidase method was used to identify HHV-6 antigen in the liver tissue. A nested polymerase chain reaction (PCR) was used to detect HHV-6 DNA in the liver tissue and mononuclear cells. Variant of HHV-6 was determined by the presence of the Hind III site in a second PCR product. RESULTS: Immunohistochemical analysis for HHV-6 antigen was negative in all the liver specimens. HHV-6 DNA was not detected in liver tissue. Virus DNA was detected in peripheral blood mononuclear cells in nine of 25 recipients. All nine HHV-6 identified in the mononuclear cells were variant B. CONCLUSIONS: HHV-6 variant B latently infects mononuclear cells but not liver tissue.
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- Drobyski W. R., Knox K. K., Majewski D., Carrigan D. R. Brief report: fatal encephalitis due to variant B human herpesvirus-6 infection in a bone marrow-transplant recipient. N Engl J Med. 1994 May 12;330(19):1356–1360. doi: 10.1056/NEJM199405123301905. [DOI] [PubMed] [Google Scholar]
- Pellett P. E., Sánchez-Martínez D., Dominguez G., Black J. B., Anton E., Greenamoyer C., Dambaugh T. R. A strongly immunoreactive virion protein of human herpesvirus 6 variant B strain Z29: identification and characterization of the gene and mapping of a variant-specific monoclonal antibody reactive epitope. Virology. 1993 Aug;195(2):521–531. doi: 10.1006/viro.1993.1403. [DOI] [PubMed] [Google Scholar]
- Salahuddin S. Z., Ablashi D. V., Markham P. D., Josephs S. F., Sturzenegger S., Kaplan M., Halligan G., Biberfeld P., Wong-Staal F., Kramarsky B. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986 Oct 31;234(4776):596–601. doi: 10.1126/science.2876520. [DOI] [PubMed] [Google Scholar]
- Schmidt C. A., Wilbron F., Weiss K., Brinkmann V., Oettle H., Lohmann R., Langrehr J. M., Neuhaus P., Siegert W. A prospective study of human herpesvirus type 6 detected by polymerase chain reaction after liver transplantation. Transplantation. 1996 Feb 27;61(4):662–664. doi: 10.1097/00007890-199602270-00027. [DOI] [PubMed] [Google Scholar]
- Singh N., Carrigan D. R., Gayowski T., Singh J., Marino I. R. Variant B human herpesvirus-6 associated febrile dermatosis with thrombocytopenia and encephalopathy in a liver transplant recipient. Transplantation. 1995 Dec 15;60(11):1355–1357. [PubMed] [Google Scholar]
- Suga S., Yazaki T., Kajita Y., Ozaki T., Asano Y. Detection of human herpesvirus 6 DNAs in samples from several body sites of patients with exanthem subitum and their mothers by polymerase chain reaction assay. J Med Virol. 1995 May;46(1):52–55. doi: 10.1002/jmv.1890460112. [DOI] [PubMed] [Google Scholar]
- Ward K. N., Gray J. J., Efstathiou S. Brief report: primary human herpesvirus 6 infection in a patient following liver transplantation from a seropositive donor. J Med Virol. 1989 Jun;28(2):69–72. doi: 10.1002/jmv.1890280203. [DOI] [PubMed] [Google Scholar]
- Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet. 1988 May 14;1(8594):1065–1067. doi: 10.1016/s0140-6736(88)91893-4. [DOI] [PubMed] [Google Scholar]
- Yoshikawa T., Suga S., Asano Y., Nakashima T., Yazaki T., Sobue R., Hirano M., Fukuda M., Kojima S., Matsuyama T. Human herpesvirus-6 infection in bone marrow transplantation. Blood. 1991 Sep 1;78(5):1381–1384. [PubMed] [Google Scholar]
- Yoshikawa T., Suga S., Asano Y., Yazaki T., Kodama H., Ozaki T. Distribution of antibodies to a causative agent of exanthem subitum (human herpesvirus-6) in healthy individuals. Pediatrics. 1989 Oct;84(4):675–677. [PubMed] [Google Scholar]