Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1999 Mar;52(3):225–227. doi: 10.1136/jcp.52.3.225

Evaluation of the BBL Crystal MRSA ID System for detection of oxacillin resistance in Staphylococcus aureus.

G Kampf 1, C Lecke 1, A K Cimbal 1, K Weist 1, H Rüden 1
PMCID: PMC501084  PMID: 10450184

Abstract

AIMS: To evaluate the BBL Crystal MRSA ID System for detection of oxacillin resistance in Staphylococcus aureus. METHODS: 52 methicillin resistant S aureus (MRSA) from five different countries and 85 methicillin susceptible S aureus (MSSA) were included. The species was confirmed by tube coagulation and detection of the clumping factor using the Staphaurex Plus. Clonal non-identity of the MRSA isolates was shown by pulsed field gel electrophoresis. MIC values (oxacillin) were determined using the Etest. Polymerase chain reaction was carried out to detect the mecA gene. The BBL Crystal MRSA ID System was carried out according to the manufacturer's instructions. RESULTS: The BBL Crystal MRSA ID System showed fluorescence in 45 of 52 MRSA (sensitivity 86.5%; negative predicitive value 92.2%), and the specificity was 97.6% (positive predicitive value 95.7%). Two of seven MRSA that failed to show fluorescence had MIC values (oxacillin) of 4 mg/litre. CONCLUSIONS: The BBL Crystal MRSA ID System is a valuable test for detecting oxacillin resistance in S aureus. Its major advantage is the short time (4-5 hours) required to perform the test when organisms are grown on tryptic soy agar or sheep blood agar. Difficulties may arise in borderline resistant isolates.

Full text

PDF
225

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker C. N., Huang M. B., Tenover F. C. Optimizing testing of methicillin-resistant Staphylococcus species. Diagn Microbiol Infect Dis. 1994 Jul;19(3):167–170. doi: 10.1016/0732-8893(94)90061-2. [DOI] [PubMed] [Google Scholar]
  2. Baker C. N., Huang M. B., Tenover F. C. Optimizing testing of methicillin-resistant Staphylococcus species. Diagn Microbiol Infect Dis. 1994 Jul;19(3):167–170. doi: 10.1016/0732-8893(94)90061-2. [DOI] [PubMed] [Google Scholar]
  3. Dutka-Malen S., Evers S., Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol. 1995 Jan;33(1):24–27. doi: 10.1128/jcm.33.1.24-27.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hiramatsu K., Kihara H., Yokota T. Analysis of borderline-resistant strains of methicillin-resistant Staphylococcus aureus using polymerase chain reaction. Microbiol Immunol. 1992;36(5):445–453. doi: 10.1111/j.1348-0421.1992.tb02043.x. [DOI] [PubMed] [Google Scholar]
  5. Jernigan J. A., Clemence M. A., Stott G. A., Titus M. G., Alexander C. H., Palumbo C. M., Farr B. M. Control of methicillin-resistant Staphylococcus aureus at a university hospital: one decade later. Infect Control Hosp Epidemiol. 1995 Dec;16(12):686–696. doi: 10.1086/647042. [DOI] [PubMed] [Google Scholar]
  6. Kampf G., Weist K., Swidsinski S., Kegel M., Rüden H. Comparison of screening methods to identify methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 1997 Apr;16(4):301–307. doi: 10.1007/BF01695635. [DOI] [PubMed] [Google Scholar]
  7. Knapp C. C., Ludwig M. D., Washington J. A. Evaluation of BBL crystal MRSA ID system. J Clin Microbiol. 1994 Oct;32(10):2588–2589. doi: 10.1128/jcm.32.10.2588-2589.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maple P. A., Hamilton-Miller J. M., Brumfitt W. World-wide antibiotic resistance in methicillin-resistant Staphylococcus aureus. Lancet. 1989 Mar 11;1(8637):537–540. doi: 10.1016/s0140-6736(89)90076-7. [DOI] [PubMed] [Google Scholar]
  9. Qadri S. M., Ueno Y., Imambaccus H., Almodovar E. Rapid detection of methicillin-resistant Staphylococcus aureus by Crystal MRSA ID System. J Clin Microbiol. 1994 Jul;32(7):1830–1832. doi: 10.1128/jcm.32.7.1830-1832.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sperber W. H., Tatini S. R. Interpretation of the tube coagulase test for identification of Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):502–505. doi: 10.1128/am.29.4.502-505.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995 Sep;33(9):2233–2239. doi: 10.1128/jcm.33.9.2233-2239.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wallet F., Roussel-Delvallez M., Courcol R. J. Choice of a routine method for detecting methicillin-resistance in staphylococci. J Antimicrob Chemother. 1996 May;37(5):901–909. doi: 10.1093/jac/37.5.901. [DOI] [PubMed] [Google Scholar]
  13. Wilkerson M., McAllister S., Miller J. M., Heiter B. J., Bourbeau P. P. Comparison of five agglutination tests for identification of Staphylococcus aureus. J Clin Microbiol. 1997 Jan;35(1):148–151. doi: 10.1128/jcm.35.1.148-151.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Witte Wolfgang, Kresken Michael, Braulke Christine, Cuny Christa. Increasing incidence and widespread dissemination of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals in central Europe, with special reference to German hospitals. Clin Microbiol Infect. 1997 Aug;3(4):414–422. doi: 10.1111/j.1469-0691.1997.tb00277.x. [DOI] [PubMed] [Google Scholar]
  15. Zambardi G., Fleurette J., Schito G. C., Auckenthaler R., Bergogne-Berezin E., Hone R., King A., Lenz W., Lohner C., Makristhatis A. European multicentre evaluation of a commercial system for identification of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 1996 Sep;15(9):747–749. doi: 10.1007/BF01691964. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES