Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1993 Jan;46(1):10–17. doi: 10.1136/jcp.46.1.10

ACP Broadsheet 135: January 1993. Isolation and identification methods for Escherichia coli O157 and other Vero cytotoxin producing strains.

H R Smith 1, S M Scotland 1
PMCID: PMC501094  PMID: 8432882

Full text

PDF
10

Images in this article

11Image
on p.11
11Image
on p.11
14Image
on p.14

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acheson D. W., Keusch G. T., Lightowlers M., Donohue-Rolfe A. Enzyme-linked immunosorbent assay for Shiga toxin and Shiga-like toxin II using P1 glycoprotein from hydatid cysts. J Infect Dis. 1990 Jan;161(1):134–137. doi: 10.1093/infdis/161.1.134. [DOI] [PubMed] [Google Scholar]
  2. Ahmed R., Bopp C., Borczyk A., Kasatiya S. Phage-typing scheme for Escherichia coli O157:H7. J Infect Dis. 1987 Apr;155(4):806–809. doi: 10.1093/infdis/155.4.806. [DOI] [PubMed] [Google Scholar]
  3. Basta M., Karmali M., Lingwood C. Sensitive receptor-specified enzyme-linked immunosorbent assay for Escherichia coli verocytotoxin. J Clin Microbiol. 1989 Jul;27(7):1617–1622. doi: 10.1128/jcm.27.7.1617-1622.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Borczyk A. A., Lior H., Thompson S. Sorbitol-negative Escherichia coli O157 other than H7. J Infect. 1989 Mar;18(2):198–199. doi: 10.1016/s0163-4453(89)91486-2. [DOI] [PubMed] [Google Scholar]
  5. Chapman P. A., Siddons C. A., Zadik P. M., Jewes L. An improved selective medium for the isolation of Escherichia coli O157. J Med Microbiol. 1991 Aug;35(2):107–110. doi: 10.1099/00222615-35-2-107. [DOI] [PubMed] [Google Scholar]
  6. Chart H., Scotland S. M., Rowe B. Serum antibodies to Escherichia coli serotype O157:H7 in patients with hemolytic uremic syndrome. J Clin Microbiol. 1989 Feb;27(2):285–290. doi: 10.1128/jcm.27.2.285-290.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Downes F. P., Green J. H., Greene K., Strockbine N., Wells J. G., Wachsmuth I. K. Development and evaluation of enzyme-linked immunosorbent assays for detection of shiga-like toxin I and shiga-like toxin II. J Clin Microbiol. 1989 Jun;27(6):1292–1297. doi: 10.1128/jcm.27.6.1292-1297.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Farmer J. J., 3rd, Davis B. R. H7 antiserum-sorbitol fermentation medium: a single tube screening medium for detecting Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol. 1985 Oct;22(4):620–625. doi: 10.1128/jcm.22.4.620-625.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gunzer F., Böhm H., Rüssmann H., Bitzan M., Aleksic S., Karch H. Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome. J Clin Microbiol. 1992 Jul;30(7):1807–1810. doi: 10.1128/jcm.30.7.1807-1810.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karmali M. A. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989 Jan;2(1):15–38. doi: 10.1128/cmr.2.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Karmali M. A., Petric M., Lim C., Cheung R., Arbus G. S. Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin. J Clin Microbiol. 1985 Oct;22(4):614–619. doi: 10.1128/jcm.22.4.614-619.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Konowalchuk J., Speirs J. I., Stavric S. Vero response to a cytotoxin of Escherichia coli. Infect Immun. 1977 Dec;18(3):775–779. doi: 10.1128/iai.18.3.775-779.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lior H., Borczyk A. A. False positive identifications of Escherichia coli O157. Lancet. 1987 Feb 7;1(8528):333–333. doi: 10.1016/s0140-6736(87)92065-4. [DOI] [PubMed] [Google Scholar]
  14. Padhye N. V., Doyle M. P. Rapid procedure for detecting enterohemorrhagic Escherichia coli O157:H7 in food. Appl Environ Microbiol. 1991 Sep;57(9):2693–2698. doi: 10.1128/aem.57.9.2693-2698.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Scotland S. M., Rowe B., Smith H. R., Willshaw G. A., Gross R. J. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol. 1988 Apr;25(4):237–243. doi: 10.1099/00222615-25-4-237. [DOI] [PubMed] [Google Scholar]
  16. Scotland S. M., Willshaw G. A., Smith H. R., Rowe B. Properties of strains of Escherichia coli belonging to serogroup O157 with special reference to production of Vero cytotoxins VT1 and VT2. Epidemiol Infect. 1987 Dec;99(3):613–624. doi: 10.1017/s0950268800066462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thomas A., Smith H. R., Willshaw G. A., Rowe B. Non-radioactively labelled polynucleotide and oligonucleotide DNA probes, for selectively detecting Escherichia coli strains producing Vero cytotoxins VT1, VT2 and VT2 variant. Mol Cell Probes. 1991 Apr;5(2):129–135. doi: 10.1016/0890-8508(91)90007-7. [DOI] [PubMed] [Google Scholar]
  18. Thompson J. S., Hodge D. S., Borczyk A. A. Rapid biochemical test to identify verocytotoxin-positive strains of Escherichia coli serotype O157. J Clin Microbiol. 1990 Oct;28(10):2165–2168. doi: 10.1128/jcm.28.10.2165-2168.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wells J. G., Davis B. R., Wachsmuth I. K., Riley L. W., Remis R. S., Sokolow R., Morris G. K. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol. 1983 Sep;18(3):512–520. doi: 10.1128/jcm.18.3.512-520.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Willshaw G. A., Smith H. R., Scotland S. M., Field A. M., Rowe B. Heterogeneity of Escherichia coli phages encoding Vero cytotoxins: comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. J Gen Microbiol. 1987 May;133(5):1309–1317. doi: 10.1099/00221287-133-5-1309. [DOI] [PubMed] [Google Scholar]
  21. Willshaw G. A., Smith H. R., Scotland S. M., Rowe B. Cloning of genes determining the production of vero cytotoxin by Escherichia coli. J Gen Microbiol. 1985 Nov;131(11):3047–3053. doi: 10.1099/00221287-131-11-3047. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES