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. 1993 Jan;46(1):45–47. doi: 10.1136/jcp.46.1.45

High incidence of type II autoantibodies in pernicious anaemia.

H M Waters 1, D W Dawson 1, J E Howarth 1, C G Geary 1
PMCID: PMC501112  PMID: 8432887

Abstract

AIMS: To investigate the incidence of type II autoantibodies to intrinsic factor in pernicious anaemia. METHODS: Three hundred and forty four serum samples submitted for intrinsic factor antibody (IFAB) analysis on clinical or laboratory grounds were tested by an established radioassay and a new enzyme linked immunosorbent assay (ELISA) method for type I and total IFAB, respectively. Sixty of these were found to be positive by ELISA; this method was used to test further, 40 samples of adequate volume for types I and II antibodies. RESULTS: Type II antibodies were detected in 39 of the 40 sera tested. A comparative analysis indicated that seven samples contained pure type II antibody, being positive for total and type II by ELISA, but negative for type I by both the ELISA and radioassay technique. CONCLUSIONS: The occurrence of type II antibody, both alone and in combination with type I, seems to be more common than has previously been recognised, and emphasises the advantage of using a technique which will detect both types of antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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