A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

Abstract

β-lactamases are the primary cause of resistance to β-lactams among members of the family Enterobacteriaceae. SHV enzymes have emerged in Enterobacteriaceae causing infections in health care in the last decades of the Twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. Likely originated from a chromosomal penicillinase of Klebsiella pneumoniae, SHV β-lactamases currently encompass a large number of allelic variants including extended-spectrum β-lactamases (ESBL), non-ESBL and several not classified variants. SHV enzymes have evolved from a narrow- to an extended-spectrum of hydrolyzing activity, including monobactams and carbapenems, as a result of amino acid changes that altered the configuration around the active site of the β -lactamases. SHV-ESBLs are usually encoded by self-transmissible plasmids that frequently carry resistance genes to other drug classes and have become widespread throughout the world in several Enterobacteriaceae, emphasizing their clinical significance.

Introduction

Thanks to their ability to inhibit cell wall biosynthesis, β-lactams remained the first-line defense against bacterial infections for over 20 years, before resistant bacteria appeared in clinical practice.

Resistance to this class of drugs can be the result of antibiotic target site alteration, prevention of antibiotic access by altered permeability or forced efflux, or antibiotic degradation (Wilke et al., 2005). The latter, represents the primary resistance mechanism in Gram-negative bacteria producing β-lactamase enzymes able to covalently bind the carbonyl moiety of the β-lactam ring and hydrolyze its amide bond (Fisher et al., 2005). Naturally occurring chromosomally located β-lactamases are quite common in Gram-negative bacteria; likely evolved from penicillin-binding proteins, when produced in small quantity they do not significantly contribute to antibiotic resistance. It was the appearance of the first plasmid-mediated β-lactamase TEM-1 (Datta and Kontomichalou, 1965) to designate the beginning of an unstoppable phenomenon in the 1960s. Ever since, the introduction of new natural or synthetic drugs to replace old ones in an attempt to limit the insurgence of antibiotic resistant bacteria triggered a chain reaction providing bacteria with a constant selective pressure driving the expansion of different resistance mechanisms (Medeiros, 1997).

In recent years β-lactamases have extensively diversified in response to the clinical use of new generations of β-lactams (penicillin, cephalosporins, carbapenems, and monobactams) leading to the need of classification schemes. Based on primary structure (Ambler, 1980), enzymatic properties and biochemical attributes (Bush et al., 1995), and the increasingly available amino acid sequences (Bush and Jacoby, 2010) four major classes (A, B, C, D) can be acknowledged. Serine β-lactamases belonging to class A are the most abundant (Philippon et al., 2016), with more than 500 enzymes, including the most clinically significant extended spectrum β-lactamases (ESBL) variants, i.e., CTX-M-, TEM-, and SHV-type enzymes (Bush and Fisher, 2011).

Although, SHV enzymes did not undergo the explosive dissemination observed for CTX-M-type variants (Canton et al., 2012), in recent years they have been found in several Enterobacteriaceae outside of the typical clinical hosts Klebsiella pneumoniae and Escherichia coli, with a rising allele variability (http://www.lahey.org/studies), and in different environmental niches. Many admirable works describing the biochemistry, the genetics and the evolution of SHV β-lactamases have appeared over the last years. The aim of this review is to provide the readers with an updated overview on SHV β-lactamases, their amino acid variants and spectrum of activity, and to describe the occurrence of plasmid-associated SHV enzymes in Enterobacteriaceae and their epidemiological significance.

Origin and diversity of the SHV family

The first bla_SHV-1 gene was identified in the 1970s in E. coli (Pitton, 1972). The encoded enzyme SHV−1 (sulfhydryl reagent variable) proved its activity against penicillins and first generation cephalosporins (Matthew et al., 1979) and was confirmed part of the conjugative plasmid p453 (Barthélémy et al., 1988; Table 1). The most likely ancestor of the plasmid-mediated SHV−1 is a chromosomal species-specific penicillinase detected in fecal K. pneumoniae isolates from neonates (Haeggman et al., 1997). The enzyme showed a typical antibiogram with penicillin rather than cephalosporin resistance and a marked inhibition by clavulanic acid. How bla_SHV-1 moved from the chromosome to the plasmid does not have a conclusive explanation since the proposed association with a transposable element (Nugent and Hedges, 1979) has not been confirmed.

Table 1.

SHV-type extended-spectrum β-lactamases.

Gene§	Accession Number	pI	Isolation		Bacterial Species	Genetic background				References
			Location	Year*		Genetic Location¥	Conjugative plasmid	Plasmid (Kb)	Other Ab genes
blaSHV-1**	AF148850	7.6	NA	1972	E. coli	p453	Yes	ND	ND	Pitton, 1972; Matthew et al., 1979
blaSHV-2	AF148851	7.6	Germany	1983	K. ozaenae	pBP60	Yes	45	ND	Kliebe et al., 1985
blaSHV-2a	X98102	7.6	Germany	1987–1988	K. pneumoniae	pZMP1	Yes	66	ND	Podbielski et al., 1991
blaSHV-3	KX092356	7.0	France	1986	K. pneumoniae	pUD18	Yes	180	ND	Nicolas et al., 1989
blaSHV-4	NA	7.8	France	1987	K. pneumoniae	P	Yes	180	ND	Péduzzi et al., 1989; Arlet et al., 1990
blaSHV-5	X55640	8.2	Chile	1987	K. pneumoniae	pAFF1	No	150	ND	Gutmann et al., 1989
blaSHV-6	Y11069.1	7.6	France	1991	K. pneumoniae	pSLH06	Yes	180	ND	Arlet et al., 1991
blaSHV-7	U20270	7.6	USA	1993	E. coli	P	Yes	10	ND	Bradford et al., 1995
blaSHV-8	U92041	7.6	USA	1990	E. coli	C	–	–	–	Rasheed et al., 1997
blaSHV-9	S82452.1	8.2	Greece	1995	E. coli; K. pneumoniae; S. marcescens	pK318-1; pE77-1; pS24-1	Yes	ND	ND	Prinarakis et al., 1996
blaSHV-11**	X98101	8.2	Switzerland	1993–1995	K. pneumoniae	P	Yes	80	ND	Nüesch-Inderbinen et al., 1997
blaSHV-12	JX268741	8.2	Switzerland	1993–1995	E. coli; K. pneumoniae	P	Yes	80	ND	Nüesch-Inderbinen et al., 1997
blaSHV-13	AF164577	7.6	Netherlands	1994	K. pneumoniae	P	Yes	170	ND	Yuan et al., 2000
blaSHV-15	AJ011428.2	ND	India	1998	E. coli	ND	ND	ND	ND	http://www.lahey.org/studies/
blaSHV-16	AF072684.2	7.6	France	1996	K. pneumoniae	P	Yes	>100	–	Arpin et al., 2001
blaSHV-18	AF132290	7.8	USA	1994	K. pneumoniae	P	Yes	80	ND	Rasheed et al., 2000
blaSHV-23	AF117747	ND	South Africa	1990	K. pneumoniae	ND	ND	ND	ND	Essack et al., 2004
blaSHV-24	AB023477	7.5	Japan	1996	E. coli	pCAZR001	Yes	150	ND	Kurokawa et al., 2000
blaSHV-27	AF293345.1	8.2	Brazil	1999	K. pneumoniae	C	–	–	ND	Corkill et al., 2001
blaSHV-30	AY661885	6.7	USA	2003	E. cloacae	P	ND	9.4	AmpC, blaTEM-1 and blaSHV-7	Szabó et al., 2005
blaSHV-31	AY277255	7.8	Netherlands	2001	K. pneumoniae	C	–	–	–	Mazzariol et al., 2007
blaSHV-34	AY036620	ND	USA	1998–2000	C. koseri; E. coli; K. pneumoniae	pOZ185	Yes	>100	ND	Heritage et al., 2003
blaSHV-38	AY079099	7.6	France	2001	K. pneumoniae	C	–	–	–	Poirel et al., 2003
blaSHV-40	AF535128	7.6	Canada	1999–2000	K. pneumoniae	ND	ND	ND	ND	Mulvey et al., 2004
blaSHV-41	AF535129	7.6	Canada	1999–2000	K. pneumoniae	ND	ND	ND	ND	Mulvey et al., 2004
blaSHV-42	AF535130	7.6	Canada	1999–2000	K. pneumoniae	ND	ND	ND	ND	Mulvey et al., 2004
blaSHV-45	AF547625	8.2	Brazil	NA	K. pneumoniae	IncA/C	ND	97-145	blaCTX-M-2 and blaSHV-27	Dropa et al., 2015
blaSHV-46	AY210887	8.2	New York	1998	K. oxytoca	P	Yes	70	blaTEM-1; blaOXY-2; blaKPC-2; blaOXA (?)	Yigit et al., 2003
blaSHV-55	DQ054528	ND	Portugal	NA	K. pneumoniae	ND	No	–	TEM1	Mendonça et al., 2006
blaSHV-57	AY223863	8.3	Taiwan	1998	E. coli	pMTY512	Yes	40–60	ND	Ma et al., 2005
blaSHV-64	DQ174304	ND	China	2000–2002	K. pneumoniae	ND	ND	ND	ND	Zuo et al., 2006
blaSHV-66	DQ174306	ND	China	2000–2002	K. pneumoniae	ND	ND	ND	ND	Zuo et al., 2006
blaSHV-70	DQ013287	7.6	China	2003–2004	E. cloacae	pEC04	Yes	ND	ND	Ling et al., 2006
blaSHV-86	DQ328802	8.2	Colombia	2003	K. pneumoniae	P	Yes	ND	ND	Espinal et al., 2010
blaSHV-90	NA	8.2	Portugal	2003	K. pneumoniae	ND	ND	ND	ND	Machado et al., 2007
blaSHV-91	NA	7.6	Portugal	2003	K. pneumoniae	ND	ND	ND	ND	Machado et al., 2007
blaSHV-98	AM941844	7.6	Algeria	2005	K. pneumoniae	ND	ND	ND	ND	Ramdani-Bouguessa et al., 2011
blaSHV-99	AM941845	7.8	Algeria	2005	K. pneumoniae	ND	ND	ND	ND	Ramdani-Bouguessa et al., 2011
blaSHV-100	AM941846	7.2	Algeria	2005	K. pneumoniae	ND	ND	ND	ND	Ramdani-Bouguessa et al., 2011
blaSHV-102	EU024485	ND	Spain	2003–2004	E. coli	ND	ND	ND	ND	Vinué et al., 2008
blaSHV-104	EU274581	7,3/8,6	Tunisia	2004	K. pneumoniae	pML2011	Yes	50	ND	Ben Achour et al., 2014
blaSHV-105	FJ194944	ND	USA	NA	K. pneumoniae	ND	ND	ND	blaSHV-1; blaSHV-5	Jones et al., 2009
blaSHV-106	AM941847	7.6	Portugal	1999	K. pneumoniae	ND	ND	ND	blaTEM-1; blaCTX-M-32	Mendonça et al., 2009
blaSHV-128	GU932590	8.6	Tunisia	2009	E. cloacae	IncFII (IS26)	Yes	100	ND	Bourouis et al., 2015
blaSHV-129	GU827715	ND	Italy	2008	E. coli	pEc6-66	ND	ND	ND	Lascols et al., 2012
blaSHV-134	HM559945	ND	Spain	2009	K. pneumoniae	IncFIIA (IS26)	Yes	75	blaVIM-1; aac(6′)-Ib; dhfrII; aadA1; catB2; blaTEM-1; aac(3′)-Iia	Sánchez-Romero et al., 2012
blaSHV-183	HG934764	ND	NA	NA	E. cloacae	ND	ND	ND	ND	http://www.lahey.org/studies/

^§Gene bla_SHV−115 was not included in the table because no information is available (http://www.lahey.org/studies).

^*Isolation or first description.

^**Non ESBL genes bla_SHV-1 and bla_SHV-11 are provided as reference.

^¥P, plasmid; C, Chromosome; when known plasmid name or Inc/rep group, and Insertion Sequences are indicated.

NA, not available; ND, not determined.

As of today, 189¹ SHV allelic variants have been described, having developed resistance to 3rd generation cephalosporin (Tzouvelekis and Bonomo, 1999), monobactam and carbapenems (Poirel et al., 2003). Only a small proportion is biochemically and/or genetically characterized (http://www.lahey.org/studies). SHV β-lactamases can be divided into three subgroups on the basis of molecular characteristics or functional properties: (i) subgroup 2b (n = 37), able to hydrolyze penicillins and early cephalosporins (cephaloridine and cephalothin) and strongly inhibited by clavulanic acid and tazobactam; (ii) subgroup 2br (n = 7), broad-spectrum β-lactamases that acquired resistance to clavulanic acid; and (iii) subgroup 2be (n = 46), comprises ESBLs that can also hydrolyze one or more oxyimino β-lactams (cefotaxime, ceftazidime, and aztreonam). More than half of these variants (n = 99) has not been classified yet due to absence of biochemical characterization.

Figure 1 illustrates a phylogenetic analysis of 149 out of the 189 SHV β-lactamase variants whose amino acid sequences were available online (http://www.lahey.org/studies), as of July 2016. Unlike other β-lactamase families (D'andrea et al., 2013; Evans and Amyes, 2014), there is no clear clustering of the different subgroups, as also mirrored by gene based analysis (Supplementary Figure S1). Among the majority of unclassified variants, subgroup 2b and the few 2br variants are scattered all over the tree. Subgroup 2be showed clustering of most of the ESBL variants (including SHV-2a, SHV-5, and SHV-12), together with few non-classified enzymes (SHV−29, SHV−152, SHV−153, SHV−160, and SHV−165). It has been proposed that SHV β-lactamases descended from an unidentified ancestor holding an extended spectrum phenotype (2be) and that subgroup 2b derived from it (Hall and Barlow, 2004). Our analysis showed that several of SHV ESBL variants were scattered along the tree with short branch lengths with neighboring 2b or unknown variants within the SHV phylogeny (i.e., SHV−40, SHV−11, and SHV−35; Figure 1), supporting the hypothesis that they evolved from multiple variants, probably within the antibiotic era. Among the non-ESBL variants, bla_SHV-11 represents one of the most successful and, together with bla_SHV-1, the likely source of evolution for the existing SHV ESBL variants. bla_SHV-11 was first identified as plasmid-encoded in clinical K. pneumoniae from Switzerland (Nüesch-Inderbinen et al., 1997) and ever since has been isolated worldwide.

Figure 1.

Maximum likelihood amino acid tree of 149 SHV-type β-lactamases. Variants whose sequence has not been released in GenBank as of July 2016, that show partial sequence or are identical to others (http://www.lahey.org/studies/) were not included in the analysis. SHV-180 and SHV-181 share the same sequence as well as SHV-121 and SHV-136. The tree was implemented in Mega version 6.06 (Tamura et al., 2013). Solid circles represent: red, extended-spectrum β-lactamases (2be; n = 46); green, broad-spectrum β-lactamases (2br, n = 5); and blue, penicillinases (2b, n = 30). Unclassified alleles are reported in black (n = 68).

Although, nearly displaced, together with TEM, by CTX-M enzymes over the years (Canton et al., 2012), 46 ESBL bla_SHV genes have been described so far (Table 1). The first report of SHV-mediated resistance to third-generation cephalosporins was in 1983 with the isolation and characterization of bla_SHV-2, encoded by plasmid pBP60 in a German clinical isolate of Klebsiella ozaenae and showing only a few nucleotide mismatches with bla_SHV-1 (Kliebe et al., 1985). In a few years four other ESBL variants were identified as plasmid-encoded in clinical K. pneumoniae, showing variable gene homologies with the bla_SHV-1 and bla_SHV-2 sequences (50–90%): bla_SHV-2a encoded by conjugative plasmid pZMP1 (Podbielski et al., 1991); bla_SHV-3 on pUD18 (Nicolas et al., 1989); bla_SHV-4, widely disseminated from France as a result of a single K. pneumoniae clone diffusion (Arlet et al., 1990, 1994); and bla_SHV-5 able to hydrolyze broad-spectrum cephalosporins and monobactams (Gutmann et al., 1989). Of these first variants, the most epidemiologically successful were bla_SHV-2a and bla_SHV-5, which will be further discussed, together with bla_SHV-2 and bla_SHV-12, in a dedicated paragraph (Section Expansion toward New Ecological Niches). Interestingly, bla_SHV-3 and bla_SHV-4 have been only sporadically detected since their first description. bla_SHV-3 seems to be geographically restricted to the USA where it was detected in E. coli of animal origin, associated with other antibiotic resistance genes such as bla_CTX-M-15, bla_CTX-M-24, bla_CMY-2, and/or bla_TEM-1 (Shaheen et al., 2011). bla_SHV-4 was identified also in Enterobacter aerogenes and Citrobacter diversus in different countries (Arpin et al., 1996; El Harrif-Heraud et al., 1997; Pitout et al., 1998).

The last two decades witnessed the appearance of several new variants (bla_SHV-7, bla_SHV-8, bla_SHV-9, bla_SHV-31, bla_SHV-38, bla_SHV-40, bla_SHV-41, and bla_SHV-42) whose dissemination was restricted to limited cases (Supplementary Table S1). A few variants seem to be geographically constrained: (i) bla_SHV-106, only described in Portuguese isolates of K. pneumoniae together with bla_TEM-1, and/or bla_CTX-M-32 (Mendonça et al., 2009); (ii) bla_SHV-55, in Portugal (Mendonça et al., 2006; Machado et al., 2007) and recently in Brazil (Dropa et al., 2015); and (iii) bla_SHV-57, in E. coli isolates from Taiwan and China (Ma et al., 2005; Tian et al., 2012). A variant worth to mention is bla_SHV-27 (Corkill et al., 2001), that has been detected on different plasmids in E. coli, K. pneumoniae and Enterobacter cloacae, associated with a vast array of antibiotic resistance genes (bla_DHA−1, bla_TEM-1, bla_TEM−1b, bla_CMY-2, bla_IMP, bla_CTX-M-14, bla_CTX-M-15, bla_SHV-12, bla_SHV-45, bla_OXA-1, dfrA5, ereA2; Muratani et al., 2006; Abbassi et al., 2008; Kiratisin et al., 2008; Duval et al., 2009; Hammami et al., 2011).

Most of SHV ESBLs (25 out of 46) are unique cases, with only one report so far. Seventeen variants are exclusively found in clinical K. pneumoniae: bla_SHV-6, bla_SHV-13, bla_SHV-16, bla_SHV-18, bla_SHV-23, bla_SHV-45, bla_SHV-64,bla_SHV-66, bla_SHV-86, bla_SHV-90, bla_SHV-91, bla_SHV-98, bla_SHV-99, bla_SHV-100,bla_SHV−104,bla_SHV-105, and bla_SHV-134. These variants have been described worldwide (Brazil, Portugal, Algeria, USA, Tunisia, Netherlands, France, South Africa, Colombia, and China) and are mostly associated to plasmids (Table 1). Some of these variants are sporadically accompanied by other antibiotic resistance genes like in the case of: (i) bla_SHV-45 encoded by an IncA/C plasmid together with bla_CTX-M-2 and bla_SHV-27 (Dropa et al., 2015); (ii) bla_SHV-134 encoded by an IncFII plasmid accompanied by a second plasmid carrying bla_VIM−1 (Sánchez-Romero et al., 2012); (iii) and bla_SHV-105, conferring reduced susceptibility to ceftazidime, ceftriaxone, and aztreonam together with bla_SHV-1, and bla_SHV-5 (Jones et al., 2009). One of the oldest variants, bla_SHV-6, was only described in France in 1991 in a K. pneumoniae clinical case (Arlet et al., 1991). It might be speculated that the 180 kb plasmid encoding bla_SHV-6 and conferring decreased susceptibility to ceftazidime and aztreonam was not stable or it reduced bacterial strain fitness preventing a successful dissemination.

Four variants have been described only in clinical E. coli: (i) bla_SHV-15, described together with bla_CMY-2 in a strain imported from India into the United Kingdom (http://www.lahey.org/studies/); (ii) bla_SHV-24, identified in Japan on a transferable 150 Kb plasmid conferring high-level resistance to ceftazidime but not cefotaxime and cefazolin (Kurokawa et al., 2000); emergence of SHV-24 might have been driven by the extensive use of ceftazidime in Japan, enabling bacterial survival in high concentrations of this drug; (iii) bla_SHV-102, recovered in a Spanish hospital and hydrolyzing cefotaxime and ceftazidime (Vinué et al., 2008); (iv) and bla_SHV-129, detected in an abscess specimen from a patient hospitalized in Italy in 2008 (Lascols et al., 2012).

bla_SHV-46 was only described on a 70 Kb conjugative plasmid also carrying bla_TEM-1 and bla_KPC−2 in a carbapenem-resistant strain of Klebsiella oxytoca from the urine of a hospitalized patient in New York (USA) in 1998 (Yigit et al., 2003). Finally, bla_SHV-34 is an interesting example of extended-spectrum β-lactamase encoded by an epidemic plasmid circulating among Citrobacter koseri, E. coli, and K. pneumoniae in the same US hospital between 1998 and 2000 (Heritage et al., 2003).

Majority of SHV ESBLs have been detected in K. pneumoniae or E. coli (Table 1). bla_SHV-30 was the first variant to be detected in an E. cloacae isolate from a blood culture from a solid-organ transplant recipient in the USA in 2003 (Szabó et al., 2005). The gene, previously described in K. pneumoniae and Salmonella (Mulvey et al., 2004; Whichard et al., 2007), was located on a 9.4 Kb plasmid and contributed together with chromosomal ampC, bla_SHV-7, and bla_TEM-1 to the antibiotic resistance profile of the E. cloacae isolate, the first of its kind producing two different SHV enzymes. Three other novel ESBL variants have been solely identified as plasmid-encoded in clinical E. cloacae: (i) bla_SHV-70, from a Chinese patient with history of ceftazidime treatment (Ling et al., 2006) and observed in other clinical Chinese settings (Liu et al., 2008); (ii) bla_SHV-128, isolated in Tunisia in 2009, located on an IncFII conjugative plasmid, and conferring resistance to all β-lactams except imipenem (Bourouis et al., 2015); (iii) and bla_SHV-183, for which additional description is not available (http://www.lahey.org/studies/).

SHV extended-spectrum β-lactamases: catalytic properties and resistance phenotype

Extended-spectrum SHV β-lactamases belong to functional group 2be, while very recently they were assigned to subclass A1 of serine β-lactamases, clustering with TEM and CTX-M enzymes among other clinically relevant β-lactamases (Bush, 2013; Philippon et al., 2016). SHV ESBLs consist of two subdomains: an α/β that includes an antiparallel five-stranded β-sheet flanked by α-helices, and an all-α-helical subdomain (Matagne et al., 1998). Similar to TEM β-lactamases (Jelsch et al., 1993), the active site is located within the cleft created by the subdomains and it contains the Ser⁷⁰ residue that mediates the nucleophilic attack on the carbonyl group of the β-lactam ring. In the vicinity of this serine residue, several conserved structural and functional amino acid motifs have been identified. These include the Ser⁷⁰XXLys (“SXXK” motif, with X representing variable amino acids), the Ser¹³⁰AspAsn (“SDN” motif), the Glu¹⁶⁶XXLysAsn (“EXXLN” motif), and the Lys²³⁴Thr/SerGly (“KTG” motif) (Bush, 2013).

Each SHV ESBL has one (SHV-2, SHV-6, SHV-8, SHV-24, SHV-27, SHV-38, SHV-41, SHV-57, SHV-98, SHV-99, SHV-102, and SHV-104) to six (SHV-128) amino acid substitutions when compared to SHV-1 (Table 2), indicating that even a single amino acid substitution is enough to convey an extended-spectrum phenotype. Therefore, we can speculate that other SHV ESBLs may still evolve from a parental SHV β-lactamase due to single spectrum-extending substitutions, although the majority of them have possibly emerged through a stepwise acquisition of several mutations (substitutions, deletions and/or insertions) from pre-existing extended-spectrum SHV variants.

Table 2.

Amino acid polymorphisms in SHV-type extended-spectrum β-lactamases.

	Amino acid position
	7	8	10	14	18	20	25	35	43	54	61	64	75	80	89	96	97	104	122	123	129	140	142	146	148	154	156	163	169	179	186	187	188	192	193	195	202	205	238	240	243	271	274	275	276	282	286
SHV-1	Y	I	L	S	T	P	A	L	R	G	R	E	V	V	E	H	Y	D	L	C	M	A	V	A	L	Q	G	D	L	D	M	A	A	K	L	T	R	R	G	E	A	S	E	R	N	I	L
SHV-2	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.
SHV-2A	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.
SHV-3	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	L	S	.	.	.	.	.	.	.	.
SHV-4	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	L	S	K	.	.	.	.	.	.	.
SHV-5	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-6	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	A	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-7	.	F	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-8	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	N	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-9	.	.	.	.	.	.	.	.	.	Del	.	.	.	.	.	.	.	.	.	.	.	R	.	.	.	.	.	.	.	.	.	.	.	N	V	.	.	.	S	K	.	.	.	.	.	.	.
SHV-11**	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-12	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-13	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	A	.	.	.	.	.	.	.	.
SHV-15	.	.	.	.	.	.	.	Q	.	.	.	.	.	M	K	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-16	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	T	H	.	.	.	.	.	.	.	.	.	.	Ins	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-18	.	F	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	A	K	.	.	.	.	.	.	.
SHV-23*	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	F	.	.	.	.	.	.	.	.	.	.	.	.	.	G	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-24	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	G	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-27	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	D	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-30	.	F	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.
SHV-31	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	K	.	.	.	.	.	.	.
SHV-34	.	F	.	.	.	.	.	.	S	.	.	G	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.
SHV-38	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	V	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-40	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	G	.	.	.	.	.	.
SHV-41	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	F	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-42	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.	.	.	.	.	.	V	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-45	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	D	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-46	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	N	.	.	S	K	.	.	.	.	.	.	.
SHV-55	F	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-57	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	R	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-64	.	.	.	.	.	.	.	Q	.	.	.	.	L	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-66	.	.	.	.	.	.	.	Q	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-70	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	V	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-86	F	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	R	.	.	.	.	.	.	.
SHV-90	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	T	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-91	.	.	.	.	.	S	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	K	.	.	.	.	.	.	.
SHV-98	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	I	.	.	.	.	.
SHV-99	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	G	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-100	F	.	.	.	.	.	.	Ins	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
SHV-102	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	A	.	.	.	.	.	.	.	.
SHV-104	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.	.	.
SHV-105	.	F	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	D	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-106*	F	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	.	.	.	.	.	.	.	.
SHV-115	.	.	.	.	.	.	.	.	.	.	H	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	K	.	.	K	.	.	.	.
SHV-128	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	R	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	T	P
SHV-129	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	L	D	.	.
SHV-134	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	E	.	.	.	.	.	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.
SHV-183	.	.	.	.	.	.	.	Q	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	Ins	.	.	.	.	.	.	.	S	K	.	.	.	.	.	.	.

Amino acid numbering is according to SHV-1 (Ambler numbering system, upper row). Dots indicate identical amino acids.

Amino acid positions for SHV-16 (96, 97, 163), SHV-86 (7), SHV-100 (7, 35), SHV-106 (7, 8) and SHV-183 (186) have been updated from what reported in the Lahey Clinic Website (http://www.lahey.org/studies/) according to GenBank sequences: bla_SHV-16, AF072684.2; bla_SHV-86, DQ328802; bla_SHV-100, AM941846; bla_SHV-106, AM941847; bla_SHV-183, HG934764.

^*not confirmed as belonging to subgroup 2be;

^**SHV-11 (Subgroup 2b) is provided as reference.

Among SHV ESBLs, amino acid substitutions are predominantly located at positions Leu³⁵, Gly²³⁸, and Glu²⁴⁰, while other less frequent but critical substitutions for the extended-spectrum phenotype occur on several amino acids including Ile⁸, Arg⁴³, Glu⁶⁴, Gly¹⁵⁶, Asp¹⁷⁹, and Arg²⁰⁵ (Table 2). Although, most of these residues are not involved directly in β-lactams hydrolysis, they result in the enhancement or relaxation of the active site, enabling it to accommodate and to efficiently react with oxyimino-β-lactams (Tzouvelekis and Bonomo, 1999). Amino acid substitutions on some of these positions (Arg⁴³, Asp¹⁷⁹, Arg²⁰⁵, Gly²³⁸, and Glu²⁴⁰) have been also associated with the expansion toward an ESBL phenotype among TEM enzymes (Knox, 1995).

Residue Leu³⁵ is located further away from the active site of class A β-lactamases and its substitution to Gln (e.g., SHV-2a, SHV-12) has been suggested to have an indirect role in enhancing the extended-spectrum capability of SHV β-lactamases (Nüesch-Inderbinen et al., 1997). In contrast, Gly²³⁸ and Glu²⁴⁰ amino acids are part of the active site lying near the R1 side chain of the β-lactam (Huletsky et al., 1993). Substitutions in Gly²³⁸ either to Ser (e.g., SHV-2, SHV-2a) or Ala (e.g., SHV-13, SHV-18) displace the β3-strand from the reactive Ser⁷⁰, resulting in a slightly expanded active site. This conformational change improves the binding to and the accommodation of newer cephalosporins with large C7 substituents, thereby expanding the substrate spectrum of these SHV ESBLs to include cefotaxime and to a lesser extent to ceftazidime (Huletsky et al., 1993; Matagne et al., 1998; Nukaga et al., 2003). It has been suggested that Glu²⁴⁰ substitutions to Arg (SHV-86) or Lys (e.g., SHV-4, SHV-5) cause the ammonium group of the long side-chains of these residues to form an electrostatic bond with the carboxylic acid group on the oxyimino-substituents of ceftazidime and aztreonam (Knox, 1995). This interaction has a dual effect on the hydrolysis of ceftazidime by improving initial binding and facilitating proper positioning within the SHV β-lactamase, whereas the hydrolysis of other β-lactams is less affected (Huletsky et al., 1993). Gly²³⁸Ser and Glu²⁴⁰Lys amino acid substitutions characterize the majority of SHV ESBLs (Table 2) and mirror those seen in extended-spectrum TEM β-lactamases. Interestingly, a plethora of extended-spectrum SHV and TEM β-lactamases exhibit higher levels of hydrolytic activity against ceftazidime than against cefotaxime (ceftazidimases) (Table 3). This phenotype was attributed to the Glu²⁴⁰Lys substitution, in contrast with most CTX-M β-lactamases lacking this critical substitution and only showing a cefotaximase activity, (Bonnet, 2004).

Table 3.

Kinetic parameters of available SHV-type extended-spectrum β-lactamases.

Enzyme	Parameter	PEN	AMP	AMX	TIC	PIP	CER	CEF	CAZ	CTX	FEP	ATM	CLA	SUL	TZB
SHV-1**	Kcat	455		900	60	570	170	10	NH	NH	>100	NH
	Km	20		90	22	60	110	26	ND	ND	>3000	ND
	Kcat/Km	23,000		10,000	2700	10,000	1500	400	ND	ND	>35	ND
	Vmax/Km	100					4	1
	Ki												0.19	1.70	0.057
	IC50												0.057	7.50	0.150
SHV-2	Ki												0.16	0.36	0.04
	IC50												0.020	0.57	0.049
	Vmax	100							6.5	70		1
	Km	3.5	12			ND		ND	24	18	NA	10
	Kcat		206			ND		ND		11	NA
	Kcat/Km		17			ND		ND		0.6	0.008
SHV-2a	Ki		13					5	72	4		3	0.08	0.47	0.027
	IC50		100					53	1	10			0.018	0.68	0.038
SHV-4	Vmax	100							52	115		5
	Km	3.5							60	25		0.5
SHV-5	Ki												0.10	0.18	0.036
	IC50												0.005	0.40	0.022
	Km	15	11					3	23	7		0.02
	Vmax/Km	100	100					51	4	7
SHV-7	Km							2.7	24	11		13
	Vmax							35	13	30		3.3
SHV-9	Vmax	100	215					58	10	24
	Km	18	12					5	18	9
	IC50												0.14	0.43
SHV-13	Km	10	28			18			91	11		77
	Vmax	100	178			136			0.38	12		0.66
	Vmax/Km	100	64			76			0.42	11		0.86
SHV-18	Vmax	100					200		13.5	26.9		< 1
	Vmax/Km	100					53		1.5	24		ND
SHV-24	Vmax		2				2.37		0.043			0.735
	Km		32				210		30			500
	Vmax/Km		0.0625				0.0113		0.000143			0.00147
	Ki		57				ND		37			ND
SHV-38$	Kcat	100		1800	10	100	40	5	110	1	3	3
	Km	13		35	14	80	150	100	3800	800	1600	5500
	Kcat/Km	7700		51,000	700	1300	270	50	30	1	2	0.5
SHV-55*	Km	5 ± 0.51		10 ± 0.14	6 ± 0.02	8 ± 0.37		9 ± 0.68	58 ± 7.40	21 ± 0.13	149 ± 2.61	5 ± 0.62
	Kcat	23 ± 0.76		23 ± 0.17	8 ± 0.00	27 ± 1.53		38 ± 3.94	9 ± 0.21	24 ± 0.34	30 ± 3.10	< 0.1
	Kcat/Km	5.3 ± 0.42		2.5 ± 0.002	1.5 ± 0.00	3.7 ± 0.03		4.4 ± 0.78	0.2 ± 0.02	1.1 ± 0.01	0.2 ± 0.02	ND
	IC50												0.02
SHV-57	Km	67							30.9
	Kcat	3.8 × 10−3							8.6 × 10−4
	Kcat/Km	5.67 × 10−5							2.78 × 10−5
	Ki												27 × 103		1.16 × 103
SHV-99*	Km	12 ± 0.11		11 ± 0.26	5 ± 0.93	13 ± 1.43		102 ± 11.38	136 ± 4.09	183 ± 0.72		196 ± 0.60
	Kcat	778 ± 616		563 ± 8	58 ± 2	563 ± 13		37 ± 2	< 0.1	< 0.1		0.5 ± 0.001
	Kcat/Km	62.3 ± 4.4		49.6 ± 1.8	13 ± 2.4	43.5 ± 6.5		0.37 ± 0.04	< 0.001	< 0.001		0.003
	IC50												0.02		0.03
SHV-104	Kcat	55			80			30		>1.8
	Km	94			10			68		>600
	Kcat/Km	0.6			8			0.44		0.003
SHV-129#*	Kcat		22.8 ± 11			1688 ± 4		26 ± 1	3.1 ± 1.5	4.8 ± 3.4	4.5 ± 0.5
	Km		46.8 ± 24			25 ± 9		12.1 ± 3.7	24 ± 3	26.7 ± 5.5	52 ± 3.5
	Kcat/Km		0.5 ± 0.7			7 ± 0.4		2.2 ± 0.3	0.13 ± 0.5	0.2 ± 0.5	0.09 ± 0.01
	Ki												0.4	0.4	0.04

Antibiotic: Penicillin (PEN); Ampicillin (AMP); Amoxicillin (AMX); Ticarcillin (TIC); Piperacillin (PIP); Cephaloridine (CER); Cephalothin (CEF); Ceftazidime (CAZ); Cefotaxime (CTX); Cefepime (FEP); Aztreonam (ATM); Clavulanic Acid (CLA); Sulbactam (SUL); Tazobactam (TZB).

Parameters are expressed as μM (K_i, IC₅₀, K_m), s⁻¹(K_cat), and μM/min (K_cat/K_m, V_max). Only antibiotics for which 3 or more SHV enzyme values were available are reported.

NA, not able to determine the rate of hydrolysis and affinity; NH, not hydrolyzed; ND, not determined.

^**Non ESBL SHV-1 is provided as reference.

^$K_cat/K_m values are expressed as mM/s.

^#K_cat/K_m values are expressed as μM/s.

^*Values (Except IC₅₀) represent mean ± standard deviation.

References: SHV-1 (Gutmann et al., 1989; Poirel et al., 2003); SHV-2 (Gutmann et al., 1989; Bradford et al., 1995; Winkler and Bonomo, 2016); SHV-2a (Podbielski et al., 1991); SHV-4 (Péduzzi et al., 1989); SHV-5 (Gutmann et al., 1989); SHV-7 (Bradford et al., 1995); SHV-9 (Prinarakis et al., 1996); SHV-13 (Yuan et al., 2000); SHV-18 (Rasheed et al., 2000); SHV-24 (Kurokawa et al., 2000); SHV-38 (Poirel et al., 2003); SHV-55 (Mendonça et al., 2006); SHV-57 (Ma et al., 2005): SHV-99 (Ramdani-Bouguessa et al., 2011); SHV-104 (Ben Achour et al., 2014); SHV-129 (Winkler and Bonomo, 2016).

Among the less frequent but critical substitutions, Ile⁸Phe in the signal sequence of the precursor of SHV ESBLs (e.g., SHV-7, SHV-18) has been associated with a more efficient β-lactamase transfer into the periplasm (Randegger et al., 2000), a proof that, beside enzymatic structure and gene expression, also the rate of transfer plays a role in resistance phenotype. On the contrary, Arg⁴³Ser (e.g., SHV-7, SHV-18) and Gly¹⁵⁶Asp (SHV-27, SHV-45, SHV-105) substitutions affect the structural arrangement of the conserved residues 64–69 and 166–170, respectively. These changes, opposite to the active site cavity (Ser⁷⁰) for the hydrolysis of the β-lactam molecules, expand the active site to accommodate bulkier cephalosporins (Knox, 1995; Corkill et al., 2001). Asp¹⁷⁹ amino acid is highly conserved among subclass A1 of serine β-lactamases and together with Arg¹⁶⁴ form a salt bridge that links the two ends of the Ω loop. Substitutions Asp¹⁷⁹Ala (SHV-6), Asp¹⁷⁹Asn (SHV-8) and Asp¹⁷⁹Gly (SHV-24) result in the elimination of the salt bridge with subsequent increase in ceftazidime resistance (Sowek et al., 1991). Several other amino acid substitutions (Table 2) have been described as either responsible for or possibly contributing to the ESBL phenotype, the detailed description of which exceeds the scope of this review.

Apart from point mutations leading to amino acid substitutions, frame shift mutations have been observed with very low occurrence among SHV ESBLs resulting in amino acid insertions (Arpin et al., 2001; Ramdani-Bouguessa et al., 2011) or deletions (Prinarakis et al., 1996). However, their role in the rising of the extended-spectrum phenotype remains unclear. SHV ESBL variants falling in this category are: (i) SHV-9, with the deletion of Gly⁵⁴ (Prinarakis et al., 1996); (ii) SHV-16, with a 5-amino acid sequence duplication (Asp^163aArgGluTrpGluThr-Asp^163bArgGluTrpGluThr) of the amino acids between 163 and 167, including Glu¹⁶⁶ in the Ω loop (Arpin et al., 2001); (iii) SHV-100, with a 13-amino acid insertion (SerGluSerGlnLeuSerGlyArgValGlyMetIleGlu) between amino acids 35 and 36 (Ramdani-Bouguessa et al., 2011); and (iv) SHV-183, with an Ala insertion between amino acids 186 and 187 (http://www.lahey.org/studies). Of note, the duplication observed in SHV-16 was shown to increase the conformational flexibility of the catalytic region facilitating the access of bulkier cephalosporins, such as ceftazidime, but resulted in enzymatic instability (Arpin et al., 2001). This finding could explain the low incidence of frame shift mutations among extended-spectrum SHV β-lactamases, due to a deleterious effect on the enzymes.

Overall, the available SHV ESBL kinetic parameters show that most of the substitutions lead to more efficient hydrolysis of oxyimino-β-lactams than penicillins, as depicted by the low K_cat values for penicillins (Table 3). While they retain their ability to hydrolyze penicillins, they are not catalytically so efficient compared to SHV-1 (Bush and Singer, 1989) and this is due to the decreased strength of the crucial hydrogen-bonding network needed for penicillin catalysis (turnover). As a consequence, since β-lactam inhibitors (clavulanic acid, sulbactam, and tazobactam) are structurally very similar to penicillin substrates, SHV ESBLs also exhibit increased susceptibility to β-lactam inhibitors compared to SHV-1 (Table 3) leading to less inhibitor required for inactivation (lower K_i and IC₅₀s; Tzouvelekis and Bonomo, 1999).

Detection

There are at least 46 known SHV-ESBL genes together with more than 150 non-ESBL or unclassified alleles to date (http://www.lahey.org/studies/). Accurate identification of these variants is essential for surveillance and for epidemiological studies of transmission mode, particularly in clinical setting, where appropriate antimicrobial therapy is critical.

A panel of different phenotypic confirmatory tests is available to determine the presence of extended-spectrum β-lactamases, including SHV-variants: minimum inhibitory concentration (MIC) determination of β-lactam with and without clavulanic acid, double disk synergy test (DDST), inhibitor potentiated disk diffusion test (IPDDT), three-dimensional test (TDT) and commercially available methods (Etest for ESBLs, Vitek ESBL cards, MicroScan panels, and BD Phoenix Automated Microbiology System (Bradford, 2001; Paterson and Bonomo, 2005). Standard microbiological procedures can take up to several days for culture, isolation and characterization and many comparative studies have shown that PCR-based methods have higher sensitivity (Bedenic et al., 2001, 2007; Singh et al., 2012), mostly due to variable levels of gene expression. Therefore, PCR and nucleotide sequence analysis (Stürenburg et al., 2003), together with various PCR-based methods, remain the gold standard for extended-spectrum β-lactamase SHV-variants identification.

Chanawong and colleagues developed a PCR-restriction fragment length polymorphism (PCR-RFLP) method to allow the identification of new SHV β-lactamases variants through detection of known mutations that alter recognition sites of restriction endonucleases (Chanawong et al., 2000). PCR-RFLP complements pre-existing PCR-single strand conformational polymorphism (PCR-SSCP) limited by partial gene amplification, thus missing potential mutation sites (M'Zali et al., 1998). PCR-RFLP can also be used in combination with restriction site insertion-PCR (RSI-PCR), a method based on primers mismatches, allowing the unambiguous identification of up to 27 SHV variants by point mutation (Chanawong et al., 2001a). Fluorescently labeled hybridization probes followed by melting curve analysis can also be used to discriminate between ESBL and non-ESBL bla_SHV genes (Randegger and Hächler, 2001). This method, termed the SHV melting curve mutation detection method, is also able to categorize SHV ESBL producers into phenotypically relevant subgroups: (i) weak ceftazidime resistance (SHV-6 and SHV-8); (ii) significant resistance to cefotaxime and ceftriaxone and moderate resistance to ceftazidime (SHV-2, SHV-2a, and SHV-3); and (iii), most effective against all expanded-spectrum cephalosporins (SHV-4, SHV-5, SHV-9, and SHV-12). Combined systems can also be developed ad hoc to rapidly screen local epidemiological settings (Chia et al., 2005). A modified SHV melting-curve mutation detection method able to distinguish between prevalent Taiwanese bla_SHV genes (SHV-1, SHV-2, SHV-2a, SHV-5, SHV-11, and SHV-12) was combined with a multiplex PCR to identify different β-lactamases genes (bla_SHV, bla_CTX-M-3-like, and bla_CTX-M-14). The design of this method can be easily adapted to other geographic areas where different ESBLs are prevalent. Multiplex real-time PCR assays for the fast detection of extended-spectrum β-lactamase and carbapenemase genes were developed with differential melting curves able to recognize up to 120 different SHV allelic variants (Singh et al., 2016).

New techniques for ESBL detection are employed alongside PCR-based methods these days. Loop-mediated isothermal amplification (LAMP) was applied to detect SHV- and other ESBL-producing bacteria in meat and proved to be more specific and sensitive than MacConkey agar or cefpodoxime disc methods (Anjum et al., 2013). Commercial DNA microarrays are also proving themselves to be accurate, with sensitivity and specificity values for ESBL detection being high. Up to 53 SHV-variants can be covered on a same array (Leinberger et al., 2010), but on the other hand some alleles may fail to be detected (i.e., SHV-12), as previously reported (Stuart et al., 2012). Because arrays have major limitations to detect novel genes or variants, PCR and sequencing remains essential. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is routinely used for bacteria identification and has been recently applied to detect ESBL-producing Enterobacteriaceae from positive blood cultures in clinical practice (Jung et al., 2014; Oviaño et al., 2014). Although, this methodology has yet to be fully validated, preliminary results show 99% sensitivity and 100% specificity, and denote a novel approach to categorize bacteria as ESBL producers.

Pyrosequencing combines standard PCR and sequencing by synthesis to rapidly determine the sequence of a target DNA region; it has been extensively used for the detection of bacterial resistance genes and bacterial community composition (Tang et al., 2016; Tian et al., 2016). This technique has been used to perform mutation analysis of bla_SHV to resolve heterogeneous sequences in clinical isolates of K. pneumoniae containing more than one SHV variant (Haanperä et al., 2008). An alternative protocol for pyrosequencing is the single-nucleotide polymorphism (SNP), ideal for the sequencing of mixed templates and determination of SNPs at the position of interest. This protocol has been applied to discriminate between eight bla_SHV variants from clinical isolates of E. coli and K. pneumoniae, reporting great reproducibility and ability to discriminate between sequences (Jones et al., 2009). A multiplex pyrosequencing assay coupled with qPCR amplification has also been recently developed to enable rapid and accurate detection of bla_SHV and bla_TEM -producing Enterobacteriaceae (Deccache et al., 2015). Overall, pyrosequencing can be a useful epidemiological tool for the exact identification of bla_SHV as a prerequisite for analyzing the spread of certain SHV variants.

Finally, the advent of whole genome sequencing (WGS) has taken differentiation of bacterial strains and identification of the associated antibiotic resistance gene cargo to another level. Aside from the phylogenetic analysis that WGS provides, the complete resistome of a strain can be unraveled as well as its mobilome, i.e., the mobile genetic elements that are associated with antibiotic resistance diffusion. Only this information can provide us with full understanding of complex genomic structures as observed, for example, in clinical K. pneumoniae genomes carrying (i) nineteen antibiotic resistance genes including bla_OXA-1 and bla_SHV-28 in the chromosome, bla_NDM-1 in a plasmid, and bla_OXA-232 in a second plasmid (Kwon et al., 2016); (ii) β-lactamase genes bla_KPC−2, bla_SHV-11, bla_TEM−169, and bla_OXA-9, together with aac(6′-)Ib, aadA2, and aph(3′-)Ia as aminoglycoside resistance encoding genes, mph(A) for macrolides, oqxA and oqxB for quinolone, catA1 for phenicol, sul1 for sulfonamide, and dfrA12 for trimethoprim (Lee et al., 2014); or (iii) six different plasmids, adding up to 0.43 Mbp, coding for six β-lactamases (bla_SHV-12, bla_OXA-9, bla_TEM-1, bla_CTX-M-2, and bla_KPC−2), together with bla_SHV-110 and adhesin-related gene clusters on the chromosome (Perreira Ramos et al., 2014).

Expansion toward new ecological niches

Over the last years the presence of antibiotics as well as antibiotic resistant bacteria has been shown outside the clinical environment, including water, soil and, most notably, food producing animals. When looking at SHV-variants distribution it is evident that in recent years, as for most extended-spectrum β-lactamases (Canton et al., 2012), their presence has been confirmed in virtually all ecological niches (Supplementary Table S1), making it more challenging to restrain antibiotic resistance diffusion. The most representative cases and variants will be discussed.

Aquatic environment

In an effort to control the release of antibiotics and antibiotic resistant bacteria in the environment, aquatic environments are being investigated worldwide, whether they be natural, drinking or wastewaters. The latter are particularly worrisome given the high prevalence of bla_SHV alleles, as observed in untreated hospital wastewater in Australia (Gündogdu et al., 2013), their possible association with determinants of quinolone and other β-lactamase resistance (Calhau et al., 2015; Osinska et al., 2016), and their relatively easy transmission to surface water through waste water treatment plant discharges (Marti et al., 2013). Studies showed that SHV types, together with CTX-M and OXA genes can be significantly decreased by biological treatments such as activated sludge processing and anaerobic digestion, although not all can be effectively eliminated (Yi et al., 2015).

Urban waters are also exposed to relatively high population densities and therefore are often unprotected from biological contaminants, with people playing a crucial role in antibiotic resistance dissemination in the environment. Unusual finding of SHV-producing Stenotrophomonas maltophilia in a swimming recreational Serbian lake and its transient presence during summer months can be considered as a proof of its anthropogenic origin, given its nature of emerging nosocomial pathogen (Novovic et al., 2015). Similar conclusions can be drawn for SHV-producing K. pneumoniae and E. cloacae isolated from a Bangladeshi lake, which receives waste water from surrounding residents, commercial buildings and clinics in Dhaka city (Haque et al., 2014), as well as for artificial water reservoirs in Poland (Wolny-Koladka and Lenart-Boron, 2016), or urban surface waters in Malaysia (Tissera and Lee, 2013). In recent surveillance studies of different rivers and lakes in Switzerland, bla_SHV-12-producing Enterobacteriaceae were isolated only in 4% of the cases (Zurfluh et al., 2013), although this variant is predominant in clinical Swiss isolates (Nüesch-Inderbinen et al., 1997). bla_SHV-12 was also detected in Enterobacteriaceae from seawater, together with tet(A) and sul2 in Portugal (Alves et al., 2014), and plasmid-encoded together with bla_TEM-1 and/or bla_CTX-M-1 in Croatia (Maravic et al., 2015). Finally, data on ESBL-producing Enterobacteriaceae isolated from drinking water is also increasing, reporting SHV alleles in rural water reservoirs in China (Zhang et al., 2015), or drinking water sources for First Nation communities in Canada (Fernando et al., 2016).

Food producing animals

Food producing animals have become subject of increasing interest after several studies demonstrated that resistant strains of animal origin can be associated to human infections, possibly through the food chain (Hasman et al., 2005). Majority of SHV variants in this reservoir belong to bla_SHV-2, bla_SHV-2a, bla_SHV-5, and bla_SHV-12 (Supplementary Table S1) owing to their successful association with conjugative plasmids (see Section Plasmid epidemiology of bla_SHV-2, bla_SHV-2a, bla_SHV-5, and bla_SHV-12).

Surveillance activities in healthy animals worldwide are generating a tremendous amount of data on ESBL distribution. Most SHV β-lactamase producers are E. coli from swine and broiler fecal samples as observed in China (Tian et al., 2012); in Spain, with bla_SHV-2 associated with bla_CTX-M-9 and bla_SHV-12 with bla_CTX-M-1, in pigs and broilers respectively (Blanc et al., 2006); in layers, cattle, and broilers but not in swine in Japan (Hiki et al., 2013; Kameyama et al., 2013); and in the Netherlands, where healthy broilers carried bla_SHV-2 in combination with bla_TEM-1 or bla_TEM−135 (Dierikx et al., 2010). Other Enterobacteriaceae like K. pneumoniae and Citrobacter freundii were positive for bla_SHV-2 or bla_SHV-12 from poultry and swine, respectively (Machado et al., 2008).

Finding ESBL producers in food producing animals is also mirrored by positive food samples worldwide, mostly retail chicken meat, as reported in Tunisia, with E. coli carrying bla_SHV-5 isolated from different butcheries, supermarkets, and local markets (Jouini et al., 2013), or Salmonella enterica carrying bla_SHV-12 in Japan (Noda et al., 2015). The cross-contamination between food producing animals and retail meat has been internationally demonstrated due to the detection of plasmid-borne SHV variants, such as bla_SHV-2 and bla_SHV-2a from Canadian chicken meat and abattoir chicken cecum (Pouget et al., 2013) or bla_SHV-2 and bla_TEM-1 in Japan (Hiroi et al., 2011), presenting the potential for horizontal transfer between Enterobacteriaceae as a high public health concern.

SHV β-lactamase producing Enterobacteriaceae have been detected also in diseased animals, as reported for septicemic broilers due to avian pathogenic E. coli encoding a remarkable array of antibiotic resistance genes (dfrA17-aadA5, bla_TEM-1, bla_CTX-M-15, bla_OXA-1, bla_SHV-2, tet(A), tet(E), qnrB2, aac(6)-Ib-cr) (Ahmed et al., 2013); for K. pneumoniae isolated from bovine mastitis in the United Kingdom (Timofte et al., 2014) and Egypt (Ahmed and Shimamoto, 2011); and for multidrug resistant S. enterica serotypes Enteritidis and Typhimurium isolated from diarrheic calves (Ahmed et al., 2009).

Finally, bla_SHV-27 is the only other SHV variant frequently reported as chromosomally located in K. pneumoniae from swine, in association with bla_SHV-11 and bla_CTX-M-1 in China (Zou et al., 2011); in E. coli isolated from farmed fish together with non ESBLs bla_SHV-1, bla_SHV-11, bla_SHV-25, and bla_SHV-26 (Jiang et al., 2012); and in opportunistic pathogens asymptomatically colonizing healthy milk cows (Hammad and Shimamoto, 2011).

Wildlife, companion animals, and vegetables

ESBL diffusion has been studied extensively in Enterobacteriaceae from humans and livestock, whereas information on antibiotic resistance in the environment is still limited. Yet, the dissemination success of bla_SHV-12 is confirmed by its introduction into the wildlife, notably in birds, as reported in Spain (Alcalá et al., 2015), the Netherlands (Veldman et al., 2013), Poland (Literak et al., 2010), and the Czech Republic (Dolejská et al., 2009). This success is likely associated to predominant avian clones and to efficient plasmids (Table 4, Figure 3) of the IncN incompatibility group, described to be more frequent in pathogenic than in commensal avian and human E. coli strains (Johnson et al., 2007). bla_SHV-5 was also detected in E. coli from several birds of prey in Portugal, alone or in associations with bla_TEM−1b (Pinto et al., 2010).

Table 4.

Plasmid epidemiology of SHV-type extended-spectrum β-lactamases.

Inc Group	Plasmid Size (Kb)*	blaSHV allele§	Other Antibiotic Resistance Genes	Bacterial Species#	Country	References
IncA/C	ND (C)	SHV-12 or SHV-2a	ND	E. coli (H)	Tunisia	Mnif et al., 2013
	150 (C)	SHV-12	blaVIM−1, aac(6′)-Ib', aadA1b, catB2, sul1, dfrA14	A. caviae (H)	Italy	Antonelli et al., 2016
	150 (NC)	SHV-12 (IS26)	blaCTX-M-14, blaDHA−1	P. mirabilis (H)	Korea	Song et al., 2011
	ND	SHV-2, SHV-5 or SHV-12	ND	E. coli (H)	France	Marcadé et al., 2009
	130 (C)	SHV-5 (IS26)	blaVEB−1, blaVIM−1, aacA7, dfrA1, aadA1, blaOXA-1, blaTEM-1, aadB, arr2, cmlA5	P. stuartii (H)	Greece	Giakkoupi et al., 2015
	97–145	SHV-45	blaCTX-M-2; blaSHV-27	K. pneumoniae (H)	Brazil	Dropa et al., 2015
	63.5–209	SHV-55	blaCTX-M-2; blaSHV-28	K. pneumoniae (H)	Brazil	Dropa et al., 2015
IncA/C-IncR	220 (C)	SHV-5	blaVEB−1, blaVIM−1, rmtB, aacA7, dfrA1, aadA1	P. stuartii (H)	Greece	Oikonomou et al., 2016
IncF	125	SHV-5 (IS26)	ND	E. coli (H)	Poland	Zienkiewicz et al., 2013
IncFIA-FIB	ND (C)	SHV-12	ND	E. coli (H)	Tunisia	Mnif et al., 2013
IncFIB	ND	SHV-12	sul3	E. coli (A)	Italy	Bortolaia et al., 2010
	95–200 (C)	SHV-2	aadA1	E. coli (A)	Canada	Pouget et al., 2013
	>23	SHV-2	ND	K. pneumoniae (H)	China	Wang et al., 2012
	ND	SHV-2	ND	E. coli (H)	France	Marcadé et al., 2009
	ND (C)	SHV-5	-	E. coli (H)	Uruguay
IncFIB10	ND	SHV-12 (IS26)	blaTEM-1	E. coli (H)	UK	Doumith et al., 2012
IncFIC	ND	SHV-5	aac(6′)-Ib', aadA1	S. marcescens (H)	Uruguay	García-Fulgueiras et al., 2011
IncF-N	ND	SHV-2	aac(6′)-Ib'	K. pneumoniae (H)	Uruguay	García-Fulgueiras et al., 2011
IncFII	ND	SHV-2 or SHV-12	ND	E. coli (H)	France	Marcadé et al., 2009
	70–80 (C)	SHV-2a (IS26)	ND	K. pneumoniae (H)	Tunisia	Elhani et al., 2010
	100 (C)	SHV-128 (IS26)	ND	E. cloacae (H)	Tunisia	Bourouis et al., 2015
IncFII-FIA	ND (C)	SHV-12	ND	E. coli (H)	Tunisia	Mnif et al., 2013
IncFII-FIA-FIB	ND (C)	SHV-12	ND	E. coli (H)	Tunisia	Mnif et al., 2013
IncFII-FIB	ND	SHV-2	ND	E. coli (H)	France	Marcadé et al., 2009
	ND (C)	SHV-2a	ND	E. coli (H)	Tunisia	Mnif et al., 2013
IncFIIk1	200–220	SHV-2, SHV-55 or SHV-106	ND	K. pneumoniae (H)	Portugal	Rodrigues et al., 2014
IncFIIk5	220	SHV-55	ND	K. pneumoniae (H)	Portugal	Rodrigues et al., 2014
IncHI2	ND (C)	SHV-2a or SHV-12	ND	E. coli (H)	Tunisia	Mnif et al., 2013
	95 (C)	SHV-12 (IS26)	ND	K. pneumoniae (H)	Tunisia	Elhani et al., 2010
	310 (C)	SHV-12	qnrB2, blaTEM-1, sul1, dfrA19, tet(D), strA, strB, aac(60)-1b	S. Senftenberg (H)	Netherlands	Veldman et al., 2010
	200 (NC)	SHV-12	tet(D)	S. Concord (H)	Netherlands	Veldman et al., 2010
	290 (C)	SHV-12	qnrB2, blaTEM−1, sul1, sul2, dfrA19, tet(D), strA, strB,	S. Concord (H)	Netherlands	Veldman et al., 2010
	180, 350, 380	SHV-12	ND	K. pneumoniae (H)	Portugal	Rodrigues et al., 2014
	400	SHV-12	ND	E. cloacae (H)	Portugal	Rodrigues et al., 2014
	320 (C)	SHV-12	qnrB2, strA/B, tet(D), clmA, sul1	S. Bredeney (H)	Spain	Herrera-Leon et al., 2011
	ND (C)	SHV-12 (IS26)	blaCTX-M-14	E. cloacae (H)	Taiwan	Chen C. M. et al., 2015
	ND (C)	SHV-12 (IS26)	blaCTX-M-3	E. cloacae(H)	Taiwan	Chen C. M. et al., 2015
IncHI2 (ST1)	300 (C)	SHV-2	ND	S. Agona or Keurmassar (H)	Senegal	Harrois et al., 2014
IncI1	ND (C)	SHV-12	ND	E. coli (H)	Bulgaria	Markovska et al., 2014
	ND	SHV-12	ND	E. coli (H)	France	Marcadé et al., 2009
	ND	SHV-12	sul3	E. coli (A)	Italy	Bortolaia et al., 2010
	19 (C)	SHV-12	–	E. coli (A)	Italy	Bortolaia et al., 2011
	340 (C)	SHV-12	ND	S. Concord (H)	Norway (Ethiopia)	Fabre et al., 2009
	95 (C)	SHV-12	–	E. coli (A)	Poland	Literak et al., 2010
	10 (NC)	SHV-12	–	S. enteritidis (H)	Spain	de Toro et al., 2013
	60 (C)	SHV-12	blaVIM−1-aacA4-dfrII- aadA1-catB2	K. pneumoniae, E. coli (H)	Spain	Tato et al., 2007
	ND (C)	SHV-12 (IS26)	blaCTX-M-3	E. cloacae (H)	Taiwan	Chen C. M. et al., 2015
	95–200 (C)	SHV-2	aadA1	E. coli, S. Heidelberg (A)	Canada	Pouget et al., 2013
	95–200 (C)	SHV-2	–	E. coli (A)	Canada	Pouget et al., 2013
	95–200 (C)	SHV-2a	aadA1, dfrA1	E. coli, S. Kiambu (A)	Canada	Pouget et al., 2013
	95–200 (C)	SHV-2a	aadA1	E. coli (A)	Canada	Pouget et al., 2013
IncI1 (ST26)	ND	SHV-12	ND	E. coli (H)	Italy	Accogli et al., 2013
	ND	SHV-12 (IS26)	ND	E. coli (A)	Portugal	Jones-Dias et al., 2016
IncI1 (ST27, CC26)	115 (C)	SHV-2	aadA2	S. Livingstone (H)	Spain	de Toro et al., 2013
IncI1 (ST29/CC26)	ND (C)	SHV-12 (IS26)	ND	E. coli (E)	Portugal	Jones-Dias et al., 2016
IncI1 (ST3)	ND	SHV-12	ND	E. coli (A)	Italy	Accogli et al., 2013
	104 (C)	SHV-12	-	E. coli (A)	Italy	Bortolaia et al., 2011
IncK	ND (NC)	SHV-12	ND	K. pneumoniae (A)	England	Timofte et al., 2014
	155	SHV-2	–	E. coli (A)	Netherlands	Dierikx et al., 2010
IncL/M	ND (C)	SHV-12	ND	E. coli (H)	Tunisia	Mnif et al., 2013
	65	SHV-12	blaKPC−2, rmtB	K. pneumoniae (H)	China	Liu et al., 2015
	65	SHV-2	ND	K. pneumoniae (H)	Portugal	Rodrigues et al., 2014
	ND (C)	SHV-2a	ND	E. coli (H)	Tunisia	Mnif et al., 2013
	60–70 (C)	SHV-2a (IS26)	ND	K. pneumoniae (H)	Tunisia	Elhani et al., 2010
	ND (C)	SHV-5 (IS26)	aacA4, aacC1, aadA1, sul1	S. Typhimurium (H)	Italy	Villa et al., 2000
	90 (C)	SHV-5	tet(A), aadA1, aacC1, aacA4, dfrA1	K. oxytoca (H)	USA	Preston et al., 2014
IncN	ND (C)	SHV-12	ND	E. coli (H)	Tunisia	Mnif et al., 2013
	ND (C)	SHV-12	ND	K. pneumoniae (H)	Bulgaria	Markovska et al., 2014
	50	SHV-12	blaVIM−1, qnrS	K. pneumoniae, E. coli (H)	Norway	Naseer et al., 2012
	50 (C)	SHV-12	blaVIM−1, qnrS	K. pneumoniae (H)	Norway	Samuelsen et al., 2011
	>23	SHV-2 (IS26)	ND	K. pneumoniae (H)	China	Wang et al., 2012
	ND (C)	SHV-2a	ND	E. coli (H)	Tunisia	Mnif et al., 2013
IncN (ST1)	ND	SHV-12	aadA2	E. coli (H)	Netherlands	Dierikx et al., 2013
IncN (ST16)	50 (C)	SHV-2	ND	S. Miami (U)	Senegal	Harrois et al., 2014
IncP	ND (C)	SHV-12 (IS26)	–	E. cloacae (H)	Taiwan	Chen C. M. et al., 2015
	95–200 (C)	SHV-2a	aadA1, dfrA1	E. coli (A)	Canada	Pouget et al., 2013
IncX3	50 (C)	SHV-12	blaKPC−2	K. pneumoniae (H)	Australia	Partridge et al., 2015
	54 (C)	SHV-12	blaNDM-1	K. pneumoniae (H)	China	Wang et al., 2014
	54 (C)	SHV-12 (IS26)	blaNDM-1	K. pneumoniae, C. freundii, E. aerogenes, E. cloacae, E. coli (H)	China	Ho et al., 2012
	60 (C)	SHV-12	blaNDM-1, blaTEM-1	E. coli (H)	China	Huang et al., 2016
	60 (C)	SHV-12	blaNDM-1	E. coli (H)	China	Huang et al., 2016
	54 (C)	SHV-12 (IS26)	blaNDM-1	E. coli (H)	China	Feng et al., 2015
	54 (C)	SHV-12 (IS26)	blaNDM−1	C. freundii (H)	China	Du et al., 2013
	50	SHV-12	qnrB7	E. coli (A)	Czech Republic	Dobiasova and Dolejska, 2016
	40	SHV-12	qnrS1	E. coli (E)	Czech Republic	Dobiasova and Dolejska, 2016
	53	SHV-12 (IS26)	blaKPC−2	K. pneumoniae (H)	France	Kassis-Chikhani et al., 2013
	50 (C)	SHV-12	blaNDM-1	E. cloacae (H)	UAE	Sonnevend et al., 2013
	50 (C)	SHV-12	blaNDM-1	E. coli (H)	UAE	Sonnevend et al., 2013
	50 (C)	SHV-12	blaNDM-1	C. freundii (H)	UAE	Sonnevend et al., 2013
	43 (C)	SHV-12 (IS26)	-	E. cloacae (H)	USA	Hargreaves et al., 2015
IncX3-N	80	SHV-12	blaTEM-1, qnrS1	E. coli (A)	Germany	Dobiasova and Dolejska, 2016
ColETp	10 (NC)	SHV-12	qnrS1	S. Typhimurium (H)	Spain	Herrera-Leon et al., 2011
R	70	SHV-12	ND	K. pneumoniae (H)	Portugal	Rodrigues et al., 2014
R+IncFIIk1	300	SHV-2	ND	K. pneumoniae (H)	Portugal	Rodrigues et al., 2014
Untypable	90–140 (C)	SHV-12 (IS26)	ND	K. pneumoniae (H)	Tunisia	Elhani et al., 2010
	ND	SHV-12 (IS26)	-	E. coli (H)	UK	Doumith et al., 2012
	ND	SHV-12 (IS26)	blaTEM−1	E. coli (H)	UK	Doumith et al., 2012
	ND	SHV-12 (IS26)	blaTEM-1, blaOXA-1, qnrS1	E. coli (H)	UK	Doumith et al., 2012
	50 (C)	SHV-12	blaNDM-1	K. pneumoniae (H)	UAE	Sonnevend et al., 2013

^*C, conjugative; NC, non-conjugative; when blank is because not determined.

^§When present, IS26 is indicated in parenthesis.

^#H, human; A, animal (mostly poultry, turkey and broilers; check reference for full description); E, environment. ND, not determined.

Emergence of Enterobacteriaceae producing β-lactamases in companion animals have been gradually reported, with CTX-M enzymes being prevalent as observed in the human scenario (Rubin and Pitout, 2014). Few studies, on both healthy and diagnostic clinical canine and feline samples, report finding other ESBL variants including bla_SHV-3 in the USA (Shaheen et al., 2011), bla_SHV-2 in Mexico (Rocha-Gracia et al., 2015), bla_SHV-12 in Italy and Poland (Carattoli et al., 2005b; Rzewuska et al., 2015) and bla_SHV-12 in association with bla_OXA-48, bla_CMY-2, bla_TEM-1, aac(6′)-Ib-cr, and qnrB2 in Germany (Stolle et al., 2013).

Lastly, SHV variants have been detected in imported vegetables in Switzerland together with bla_SHV-12 for the first time in the opportunistic foodborne pathogen Cronobacter sakazakii whose potential to cause bacteremia and meningitis is an actual concern (Zurfluh et al., 2015). Similar results were observed in vegetables collected in South Korea (Kim et al., 2015), salads in the Netherlands (Reuland et al., 2014), and Spain (Egea et al., 2011), displaying a new route of introduction for ESBLs and pathogenic Enterobacteriaceae.

Plasmid epidemiology of bla_SHV−2, bla_SHV−2a, bla_SHV−5, and bla_SHV−12

The role of plasmids in the successful spread of β–lactamase genes has been extensively described (Carattoli, 2009, 2013) and, among the SHV family, it finds its best examples in bla_SHV-2, bla_SHV-2a, bla_SHV-5, and bla_SHV-12. Combination of these alleles with different dissemination machineries has brought the enzymes to reach diverse niches worldwide (Figure 2). Plasmids belonging to seven replicon types (A/C, F, HI2, I1, L/M, N, X3) have been shown to drive the epidemiology of these four predominant SHV ESBLs, although their distribution varies on the plasmid families (Table 4). Other rep families that have been only incidentally associated with extended-spectrum SHV β–lactamases include the ColE, K, P, and R (Table 4).

Figure 2.

Worldwide distribution of plasmid families encoding bla_SHV-2, bla_SHV-2a, bla_SHV-5, and bla_SHV-12. Inc/rep types are represented in different colors; diverse symbols depict human, bovine, porcine, poultry, wildlife, soil, or wastewater sources. For a complete full reference list see Table 4.

IncA/C

bla_SHV-12 has been identified on mostly conjugative broad-host range IncA/C plasmids in a variety of bacterial species, including E. coli, Proteus mirabilis and Aeromonas caviae, isolated from clinical samples in Tunisia, France, Korea and Italy (Marcadé et al., 2009; Song et al., 2011; Mnif et al., 2013; Antonelli et al., 2016). E. coli isolates recovered from clinical specimens encoding bla_SHV-2, bla_SHV-2a, and bla_SHV-5 have been also identified in Tunisia and France (Marcadé et al., 2009; Mnif et al., 2013), whereas Providencia stuartii isolates encoding bla_SHV-5 on either IncA/C or multireplicon IncA/C-R plasmids have been reported from different outbreaks in Greece (Giakkoupi et al., 2015; Oikonomou et al., 2016). Interestingly, these IncA/C plasmids (130–220 Kb) often carried multiple resistance genes, conferring multidrug resistant phenotypes (Giakkoupi et al., 2015; Antonelli et al., 2016; Oikonomou et al., 2016), resulting in the proliferation of the SHV ESBLs by co-selection.

IncF

Plasmids belonging to the narrow-host range IncF group, including plasmids with fused replicons, have been reported to accommodate bla_SHV-12 among clinical E. coli isolates from France (IncFII), Tunisia (IncFIA-FIB, IncFII-FIA, IncFII-FIA-FIB) and United Kingdom (IncFIB), but also among food-producing animals from Italy (IncFIB) (Marcadé et al., 2009; Bortolaia et al., 2010; Doumith et al., 2012; Mnif et al., 2013). IncF plasmids account for the dissemination of bla_SHV-2 gene among E. coli from both clinical specimens in France (IncFIB, IncFII, IncFII-FIB) and food-producing animals (avian and porcine sources) in Canada (IncFIB), as well as in clinical K. pneumoniae isolates belonging to ST654 and ST15 from China (IncFIB) and Portugal (IncFII), respectively (Marcadé et al., 2009; Wang et al., 2012; Pouget et al., 2013; Rodrigues et al., 2014). Finally, clinical E. coli and K. pneumoniae from Tunisia were found to encode bla_SHV-2a (Elhani et al., 2010; Mnif et al., 2013), clinical E. coli from Poland encoded bla_SHV-5 on IncF plasmids, as well as clinical K. pneumoniae and Serratia marcescens from Uruguay (García-Fulgueiras et al., 2011; Zienkiewicz et al., 2013), whereas the same plasmids have been associated with less prevalent SHV ESBLs (bla_SHV-55 and bla_SHV-106) in clinical K. pneumoniae isolates from Portugal (Rodrigues et al., 2014).

IncHI2

In contrast with the IncA/C and IncF plasmids, the broad-host range IncHI2 group is responsible mainly for the dissemination of bla_SHV-12, although this group has been found incidentally to also accommodate bla_SHV-2a (Mnif et al., 2013). Plasmids of this group varying in sizes (95–400 Kb) have been reported to encode bla_SHV-12 in various bacterial species, such as E. coli, K. pneumoniae, E. cloacae, and at least three S. enterica serotypes (Bredeney, Concord, and Senftenberg) from human specimens with diverse geographical origin (Netherlands, Portugal, Spain, Taiwan, Tunisia; Elhani et al., 2010; Veldman et al., 2010; Herrera-Leon et al., 2011; Mnif et al., 2013; Rodrigues et al., 2014; Chen C. M. et al., 2015). Apart from bla_SHV-12, some of these conjugative plasmids have been reported to co-encode for other resistance genes, including additional SHV ESBLs (bla_CTX-M-3, bla_CTX-M-14; Veldman et al., 2010; Chen C. M. et al., 2015).

IncI1

The IncI1 group, consisting of narrow-host range mostly conjugative plasmids, ranks amongst the top facilitators of bla_SHV-2, bla_SHV-2a, and bla_SHV-12 genes. The range of bacterial species they have encountered is limited to E. coli, K. pneumoniae, E. cloacae, and the S. enterica serotypes Concord, Enteritidis, Heidelberg and Kiambu. Nevertheless, IncI1 plasmids (19–340 Kb) occur in very diverse settings: bla_SHV-2- and bla_SHV-12-encoding isolates from human infections (Bulgaria, France, Italy, Spain, Taiwan; Tato et al., 2007; Marcadé et al., 2009; Accogli et al., 2013; de Toro et al., 2013; Markovska et al., 2014; Chen C. M. et al., 2015) and colonization (Ethiopia) (Fabre et al., 2009); bla_SHV-2-, bla_SHV-2a–, and bla_SHV-12-encoding isolates from poultry (Canada, Italy, Portugal; Bortolaia et al., 2010, 2011; Accogli et al., 2013; Pouget et al., 2013; Jones-Dias et al., 2015), bla_SHV-2- and bla_SHV-2a-encoding isolates from pigs (Canada) (Pouget et al., 2013); bla_SHV-12-encoding isolates from aquatic birds (Poland) (Literak et al., 2010); and bla_SHV-12-encoding isolates from farming soil (Portugal) (Jones-Dias et al., 2016). Remarkably, bla_SHV-12 on IncI1 plasmids belonging to pST26 have been identified among E. coli isolates of human and animal origin (Accogli et al., 2013; Jones-Dias et al., 2015), indicating the potential transmission of these bla_SHV-12-encoding vehicles from human to animals and/or vice versa.

IncL/M and IncN

The broad-host range IncL/M and IncN plasmids contribute to a lesser extent to the epidemiology of bla_SHV-2, bla_SHV-2a, bla_SHV-5, and bla_SHV-12 than the above-mentioned families. IncL/M plasmids (60–90 Kb) carrying SHV ESBL genes have been reported only among E. coli, K. pneumoniae, K. oxytoca, and S. enterica serotype Typhimurium of human origin in Portugal (bla_SHV-2), Tunisia (bla_SHV-2a, bla_SHV-12), Italy (bla_SHV-5), USA (bla_SHV-5), and recently in China (bla_SHV-12) (Villa et al., 2000; Elhani et al., 2010; Mnif et al., 2013; Preston et al., 2014; Rodrigues et al., 2014; Liu et al., 2015). The same bacterial species mostly from human sources carry IncN plasmids (~50 Kb) encoding bla_SHV-2 (China, Senegal), bla_SHV-2a (Tunisia) or bla_SHV-12 (Bulgaria, Netherlands, Norway, Tunisia; Samuelsen et al., 2011; Naseer et al., 2012; Wang et al., 2012; Dierikx et al., 2013; Mnif et al., 2013; Harrois et al., 2014; Markovska et al., 2014). Interestingly, the presence of IncN (pST1) plasmids encoding bla_SHV-12 has been reported among E. coli from human and animal sources (Dierikx et al., 2013), mirroring the situation for IncI1 plasmids and underscoring the contribution of this plasmid family in the transmission of bla_SHV-12 within or between these niches.

IncX3

The IncX3 plasmid subgroup consists of narrow-host range plasmids and plays an important role in the exclusive dissemination of bla_SHV-12. Conjugative plasmids (40–60 Kb) of this subgroup have been identified in diverse bacterial species (E. coli, K. pneumoniae, C. freundii, E. aerogenes, E. cloacae), sources (human, animal, environment) and geographical areas (Australia, China, Czech Republic, France, United Arab Emirates, US; Ho et al., 2012; Du et al., 2013; Kassis-Chikhani et al., 2013; Sonnevend et al., 2013; Wang et al., 2014; Feng et al., 2015; Hargreaves et al., 2015; Partridge et al., 2015; Dobiasova and Dolejska, 2016; Huang et al., 2016). Interestingly, the majority of these plasmids appear to co-harbor carbapenemase genes (bla_KPC-2, bla_NDM-1), whereas the co-localization of SHV ESBL and carbapenemase genes was reported only on IncA/C or IncA/C-R (bla_VIM−1), IncL/M (bla_KPC−2), and IncN (bla_VIM−1) plasmids (Samuelsen et al., 2011; Naseer et al., 2012; Giakkoupi et al., 2015; Oikonomou et al., 2016), enhancing the plasmid potential maintenance among bacterial populations and the subsequent preservation and dissemination of the SHV ESBL genes.

Miscellaneous plasmids

bla_SHV-12 has been incidentally found on: (i) a ColE plasmid from S. enterica serotype Typhimurium DT104b in Spain (Herrera-Leon et al., 2011); (ii) an IncK plasmid from K. pneumoniae in the United Kingdom (Timofte et al., 2014); (iii) an IncP plasmid from E. cloacae in Taiwan (Chen C. M. et al., 2015); and (iv) a plasmid assigned to the R replicon type from K. pneumoniae in Portugal (Rodrigues et al., 2014). E. coli and K. pneumoniae encoding bla_SHV-2 on IncK plasmids were recovered from animal and human sources in the Netherlands and in Uruguay, respectively (Dierikx et al., 2010; García-Fulgueiras et al., 2011). IncP plasmids encoding bla_SHV-2a from animals in Canada and bla_SHV-5 from human in Uruguay have also been reported (García-Fulgueiras et al., 2011; Pouget et al., 2013). Finally, a number of reports highlight the presence of bla_SHV-12 on mostly conjugative non-typeable plasmids, according to the PCR-based replicon-typing scheme (Carattoli et al., 2005a). These plasmids of human origin, varying between 50 and 140 Kb in size, were mostly detected among E. coli from the United Kingdom (Doumith et al., 2012) and K. pneumoniae from Tunisia (Elhani et al., 2010) and United Arab Emirates (Sonnevend et al., 2013), underscoring that their dissemination is wider than we know.

IS26 role in bla_SHV mobilization

Analysis of the sequences bracketing several SHV ESBL genes (bla_SHV-2, bla_SHV-2a, bla_SHV-5, bla_SHV-12, bla_SHV-106, and bla_SHV-134) among Gram-negative bacteria, including Enterobacteriaceae and non-fermenters, revealed that these β-lactamase genes are mostly associated with the IS26 element (Table 4, Supplementary Table S1). Beside SHV ESBLs, this member of the IS6 insertion sequence family (Mahillon and Chandler, 1998), has been associated with a plethora of resistance genes (Allard et al., 1993; Miriagou et al., 2005; Post and Hall, 2009; Cain et al., 2010; Hordijk et al., 2011) and has been found to contribute to their expression by supplying a promoter -35 box that can be coupled with a -10 box in the adjacent DNA (Lee et al., 1990; Cain and Hall, 2011). In contrast to other insertions sequences, it has been suggested that IS26 transposes preferentially within plasmids rather than into the chromosome (He et al., 2015), possibly explaining the linkage of IS26 and the four predominant SHV ESBL genes with IncA/C (bla_SHV-5, bla_SHV-12), IncF (bla_SHV-2a, bla_SHV-5, bla_SHV-12), IncHI2 (bla_SHV-12), IncI1 (bla_SHV-12), IncL/M (bla_SHV-2a, bla_SHV-5), IncN (bla_SHV-2), IncP (bla_SHV-12), IncX3 (bla_SHV-12), and non-typeable (bla_SHV-12) plasmids (Table 4). Similarly to most antibiotic resistance genes, IS26-mediated mobilization of SHV ESBL genes on conjugative plasmids facilitated their subsequent intra- and inter-species dissemination (Table 4). Available sequences of transposons flanked by copies of intact and/or truncated IS26 elements (Figure 3) and coding for SHV ESBL genes show the presence of other co-linear genes originating from the chromosome of K. pneumoniae (i.e., fucA, ygbI, ygbK, ygbJ, ygbM, deoR; Ho et al., 2012; Wang et al., 2012; Du et al., 2013; Kassis-Chikhani et al., 2013; Preston et al., 2014; Chen C. M. et al., 2015; Feng et al., 2015; Giakkoupi et al., 2015), likely underscoring the involvement of IS26 in the mobilization of bla_SHV from the chromosome of K. pneumoniae, as previously suggested (Haeggman et al., 1997).

Figure 3.

Schematic representation of bla_SHV genetic surroundings and IS26 association. Common occurring features are color coded. Map is not to scale. References: IncN (Wang et al., 2012); IncA/C (Giakkoupi et al., 2015); IncL/M (Preston et al., 2014); IncHI2, IncI1, IncP (Chen C. M. et al., 2015); IncX3 (Ho et al., 2012; Du et al., 2013; Kassis-Chikhani et al., 2013; Feng et al., 2015).

Outside of the enterobacteriaceae and a few peculiar SHV ESBLs

SHV β–lactamases have virtually invaded all human, environmental and animal sceneries, mostly associated to Enterobacteriaceae. In recent years, the first reports of alternative bacterial hosts have been described, notably in Aeromonads, ubiquitous in aquatic habitats and occasionally able to cause human infections. bla_SHV-12 was detected, in association with bla_FOX-2 and bla_CTX-M-15, on the chromosome of the foodborne pathogens A. caviae and Aeromonas hydrophila from wild-growing mussels from Croatia (Maravić et al., 2013). The first identification of plasmid-encoded SHV-12, together with VIM-1, occurred in clinical A. caviae accountable for a newborn bloodstream infection (Antonelli et al., 2016). The coproduction of these enzymes highlights the potential risks for public health and the role of Aeromonads as reservoirs and dissemination tools of resistance determinants in both environmental and clinical settings.

Occasionally, SHV ESBL-producing Pseudomonas aeruginosa can be detected in nosocomial settings and can pose a serious threat as healthcare-associated infection in many regions of the world. bla_SHV-2a was first identified on the chromosome of a 1995 clinical P. aeruginosa strain, with high sequence homology to plasmid pMPA2a from K. pneumoniae indicating a likely enterobacterial gene origin (Naas et al., 1999). Subsequent studies demonstrated the insertion of bla_SHV alleles into P. aeruginosa chromosome: bla_SHV in China (Chen Z. et al., 2015) and Iran (Shahcheraghi et al., 2009); bla_SHV-5 (Poirel et al., 2004) and bla_SHV-12 (Neonakis et al., 2003) in Greece; and bla_SHV-2a in Tunisia (Mansour et al., 2009) and France (Hocquet et al., 2010; Jeannot et al., 2013). The role of IS26 in the mobilization of bla_SHV-12 was demonstrated by the chromosomal insertion of an IS26 composite transposon (>24 kb) thanks to the co-mobilization of antibiotic resistance aac(6′)-Ib, which confers amikacin resistance, likely occurred during the clinical course of a burn infection, immediately after amikacin administration (Uemura et al., 2010). bla_SHV-5, bla_SHV-11, bla_SHV-12 were also detected in different combinations, together with bla_TEM-1b, on various plasmids in Thailand (Chanawong et al., 2001b).

Finally, one of the most effective associations outside of the Enterobacteriaceae is with Acinetobacter baumannii, contributing to the worrisome spread of ESBL-producing strains especially in clinical outbreaks (Blackwell et al., 2016). Plasmid transfer from nosocomial SHV-encoding Enterobacteriaceae seems to be responsible for this phenomenon, as observed for SHV-12 in the Netherlands (Naiemi et al., 2005), or SHV-5 in the USA, a country where this variant is the most prevalent ESBL gene in Enterobacteriaceae (Naas et al., 2007).

Among all ESBL SHV β-lactamases, few enzymes deserve special consideration because of their unique enzymatic features.

SHV-38 is a unique allelic variant of the SHV family to have an expanded-spectrum to carbapenems. It was first described in K. pneumoniae from France (Poirel et al., 2003) and it holds a point mutation (Ala¹⁴⁶Val) compared to the chromosome-encoded SHV-1. Among all 46 available SHV ESBL variants, only SHV-38 possess the Ala¹⁴⁶Val substitution (Table 2), likely inducing subtle structural conformational changes favoring imipenem but not meropenem hydrolysis (Walther-Rasmussen and Høiby, 2007).

SHV-129 is a novel clinically acquired variant identified in 2012 from an Italian E. coli isolate (Table 1; Lascols et al., 2012) and it represents an interesting example of enzyme evolution due to antibiotic pressure. Alongside two well-known amino acid substitutions (Gly²³⁸Ser, Glu²⁴⁰Lys), SHV-129 contains new substitutions, Arg²⁷⁵Leu and Asn¹⁴⁶Asp). The latter was recently demonstrated to be the first global suppressor substitution identified in the SHV β-lactamase family (Winkler and Bonomo, 2016), likely helping in protein stabilization and functionality, as well as in the ability of the enzyme to acquire additional substitutions. It is also proposed that due to the increasing clinical use of cefepime, SHV-129 might have evolved from SHV-2 or SHV-5 in an alternative conformation to expand its spectrum to hydrolyze cefepime, as mirrored by the kinetic parameters of the three enzymes (Table 3).

Finally, SHV-2 can be located on both chromosome and self-transmissible plasmids (Table 4; Supplementary Table S1). Association of bla_SHV-2 with RCS47, a P1-like bacteriophage that infects and lysogenizes E. coli and several other enteric bacteria, was recently reported (Billard-Pomares et al., 2014). bla_SHV-2 is flanked by two IS26 elements that likely drove the insertion in the phage backbone. The P1-like prophages were found with high prevalence in natural E. coli of both animal and human origin, including ESBL-producing isolates. This kind of association was already reported for other β-lactamases (bla_TEM, bla_CTX-M, and mecA) from river and urban sewage water (von Wintersdorff et al., 2016), suggesting that bacteriophages might play a wider role in favoring horizontal transfer of antibiotic resistance determinants than initially thought (Muniesa et al., 2013).

Concluding remarks

Tzouvelekis and Bonomo suggested than “it will not be surprising if (SHV) enzymes will continue to expand their substrate spectrum as long as the current antibiotics, or novel ones derived from the basic β-lactam structure, are used” (Tzouvelekis and Bonomo, 1999). In the last two decades we observed the appearance of multiple SHV-type variants, with few ones significantly expanding their substrate. One exception is represented by SHV-38, the only known SHV allelic variant able to hydrolyze carbapenems (Poirel et al., 2003), a feature that has not been associated with any TEM or CTX-M enzyme. In this image resides the fate of SHV extended β-lactamases, unable to undergo the dominant propagation observed, for instance, for the CTX-M family but yet contributing to β-lactam resistance in a not negligible way.

The persistence of SHV enzymes in the bacterial community might also be secured by co-selection with emerging resistance genes. Association of bla_SHV-12 with IncX3 plasmids carrying carbapenemase genes bla_KPC−2 and bla_NDM has been observed in recent years (Table 4) and it seems to be a phenomenon occurring in clinical carbapenem-resistant Enterobacteriaceae worldwide (Kassis-Chikhani et al., 2013; Sonnevend et al., 2013; Partridge et al., 2015; Huang et al., 2016). As highlighted in this review, the association of successful variants bla_SHV-2, bla_SHV-2a, bla_SHV-5, and bla_SHV-12 with different families of conjugative plasmids (IncA/C, IncF, IncHI2) might also underlie the colonization of virtually all ecological niches encompassing food producing animals, aquatic environment, wildlife, companion animals, and vegetables. Plasmid mediated transfer from nosocomial Enterobacteriaceae enabled SHV dispersion toward alternative bacterial hosts such as the emerging nosocomial pathogens of aquatic origin S. maltophilia and A. caviae, or contributed to the worrisome spread of ESBL-producing strains of A. baumannii and P. aeruginosa. Most interestingly, the ubiquitous presence of SHV ESBL genes and plasmids is suggestive for transmission in human, animals, and the environment, most likely through the food chain, highlighting the potential risks for public health and endorsing a one health research approach.

Overall, SHV ESBL enzymes have kept a stable role in antibiotic resistance over the years. Allele diversification is still occurring, the latest variant being identified in E. cloacae in 2014 (bla_SHV-183), and effective associations with new genetic platforms are taking place helping expansion toward novel bacterial hosts and reservoirs.

Author contributions

The paper was written by AL and DC, and reviewed by DM. All authors discussed, read, contributed to and approved the final manuscript.

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Supplementary material

The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb.2016.01374