Abstract
Epidermal growth factor receptor (EGFR) is a sensitive prognostic indicator in women with breast cancer. A new technique for the detection of EGFR on breast cancer imprints is described and evaluated in relation to immunohistochemistry on frozen sections. The standard streptavidin-biotin method using the R1 antibody on frozen sections was modified for use on tumour imprints. In the first group (group 1 imprints) 43 tumours were assessed by frozen section and imprint staining. The group 1 imprint method had a sensitivity of 50% and a specificity of 89% compared with the frozen sections. Further modifications were made for the following 32 tumours (group 2) and this method had a much improved sensitivity of 94% while specificity was preserved. This method is simple and could be performed in any hospital histopathology department.
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- Carpenter G. Binding assays for epidermal growth factor. Methods Enzymol. 1985;109:101–110. doi: 10.1016/0076-6879(85)09079-6. [DOI] [PubMed] [Google Scholar]
- Lemoine N. R., Hughes C. M., Barton C. M., Poulsom R., Jeffery R. E., Klöppel G., Hall P. A., Gullick W. J. The epidermal growth factor receptor in human pancreatic cancer. J Pathol. 1992 Jan;166(1):7–12. doi: 10.1002/path.1711660103. [DOI] [PubMed] [Google Scholar]
- Nicholson S., Halcrow P., Sainsbury J. R., Angus B., Chambers P., Farndon J. R., Harris A. L. Epidermal growth factor receptor (EGFr) status associated with failure of primary endocrine therapy in elderly postmenopausal patients with breast cancer. Br J Cancer. 1988 Dec;58(6):810–814. doi: 10.1038/bjc.1988.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicholson S., Sainsbury J. R., Halcrow P., Chambers P., Farndon J. R., Harris A. L. Expression of epidermal growth factor receptors associated with lack of response to endocrine therapy in recurrent breast cancer. Lancet. 1989 Jan 28;1(8631):182–185. doi: 10.1016/s0140-6736(89)91202-6. [DOI] [PubMed] [Google Scholar]
- Siegall C. B., FitzGerald D. J., Pastan I. Selective killing of tumor cells using EGF or TGF alpha-Pseudomonas exotoxin chimeric molecules. Semin Cancer Biol. 1990 Oct;1(5):345–350. [PubMed] [Google Scholar]
- Skoog L., Humla S., Isaksson S., Tani E. Immunocytochemical analysis of receptors for estrogen and progesterone in fine-needle aspirates from human breast carcinomas. Diagn Cytopathol. 1990;6(2):95–98. doi: 10.1002/dc.2840060205. [DOI] [PubMed] [Google Scholar]
- Toi M., Nakamura T., Mukaida H., Wada T., Osaki A., Yamada H., Toge T., Niimoto M., Hattori T. Relationship between epidermal growth factor receptor status and various prognostic factors in human breast cancer. Cancer. 1990 May 1;65(9):1980–1984. doi: 10.1002/1097-0142(19900501)65:9<1980::aid-cncr2820650917>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
- Waterfield M. D., Mayes E. L., Stroobant P., Bennet P. L., Young S., Goodfellow P. N., Banting G. S., Ozanne B. A monoclonal antibody to the human epidermal growth factor receptor. J Cell Biochem. 1982;20(2):149–161. doi: 10.1002/jcb.240200207. [DOI] [PubMed] [Google Scholar]