Table 1.

Primer sets used for quantitative RT‐PCR

Gene	Forward primer	Reverse primer
HPRT	5′‐GAAAGACTTGCTCGAGATGTCATG‐3′	5′‐CACACAGAGGGCCACAATGT‐3′
IFN‐β	5′‐CGGACTTCAAGATCCCTATGGA‐3′	5′‐TGGCAAAGGCAGTGTAACTCTTC‐3′
IL‐6	5′‐TCGGAGGCTTAATTACACATGTTC‐3′	5′‐TGCCATTGCACAACTCTTTTCT‐3′
TNF‐α	5′‐TCCCAGGTTCTCTTCAAGGGA‐3′	5′‐GGTGAGGAGCACGTAGTCGG‐3′
CCL2	5′‐GCAGCAGGTGTCCCAAAGAA‐3′	5′GGTCAGCACAGACCTCTCTCTTG‐3′
CXCL10	5′‐GGCCATAGGGAAGCTTGAAAT‐3′	5′‐TCGTGGCAATGATCTCAACAC‐3′
TLR2	5′‐CCCTGTGCCACCATTTCC‐3′	5′‐CCACGCCCACATCATTCTC‐3′
TLR3	5′‐TGGGCTGAAGTGGACAAATCT‐3′	5′‐TGCCGACATCATGGAGGTT‐3′
TLR4	AGCTTCAATGGTGCCATCATT	CCAGGTGCTGCAGCTCTTCT
TLR7	5′‐CAGTGAACTCTGGCCGTTGA‐3′	5′‐CAAGCCGGTTGTTGGAGAA‐3′
MHV‐N	5′‐TGGAAGGTCTGCACCTGCTA‐3′	5′‐TTTGGCCCACGGGATTG‐3′
RIG‐I	5′‐GCCAGAGTGTCAGAATCTCAGTCAG‐3′	5′‐GAGAACACAGTTGCCTGCTGCTCA‐3′
MDA‐5	5′‐GCCCTCTCCTTCCTCTGAGACT‐3′	5′‐GCTGGAGGAGGGTCAGCAA‐3′
IL‐33	5′‐GCTGCGTCTGTTGACACATTG‐3′	5′‐GGGAGGCAGGAGACTGTGTTAA‐3′
Fgl‐2	5′‐ CGTTGTGGTCAACAGTTTGGA‐3′	5′‐ GATGTTGAACCGGCTGTGACT‐3′
CXCL1	5′‐CCGAAGTCATAGCCACACTCAA‐3′	5′‐CAAGGGAGCTTCAGGGTCAA‐3′