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. 2016 Jul 22;204(1):205–220. doi: 10.1534/genetics.116.189258

Figure 1.

Figure 1

Deletion of DMA1 and DMA2 causes synthetic defects in cells lacking the formin Bni1 and weakens the actin cable network. (A and B) Serial dilutions of strains with the indicated genotypes were spotted on YEPD plates and incubated at the indicated temperatures. (C) Cultures from the indicated strains all expressing GFP-Cdc12 were grown in SD medium at 25° and shifted to 37° for 2 hr. Representative GFP-Cdc12 images from Z-stack max-projection (11 planes at 0.3-μm spacing) and DIC from a single plane at 37° are shown. The dot plot indicates average diameters of septin rings in the different strains after 2 hr of incubation at 37° (n = 35). Green arrowheads indicate ectopic septin assemblies or abnormal rings. Bar, 5 μm. (D) F-actin was stained with Alexa-546 phalloidin on fixed asynchronous cells grown at 30°. Arrowheads indicate actin cables. Bar, 5 μm. Graphs indicate the percentage of medium-sized budded cells with ≥3 or <3 actin cables or with actin cables extending more or less than half the size of the mother cell (n ≥ 200; error bars: SD). (E) Serial dilutions of strains with the indicated genotypes were spotted on YEPD plates either lacking or containing 5 μM LatB and incubated at 25°.