Figure 4.

The effect of individual siRNA duplexes for nine candidate genes from the siRNA screen in RAW264.7 macrophages on LTA-induced IL-6 production. Four siRNA duplexes were transfected individually for each of the eight genes whose inhibition resulted in decreased IL-6 production and one gene whose inhibition resulted in higher IL-6 production in the screen presented in Figure 3. Means of four independent measurements with error bars representing SEMs are plotted; green lines indicate threshold for twofold reduction and red twofold increases in IL-6 response as a result of gene inhibition. ^#P < 0.001 and ^P < 0.01 by one-group two-tailed t-test testing for deviation from 1.