Correction: Procollagen C-Proteinase Enhancer 1 (PCPE-1) as a Plasma Marker of Muscle and Liver Fibrosis in Mice

Fig 2 is incomplete as Panel C is missing. The authors have provided the complete Fig 2 here.

Fig 2. Diaphragms from mdx mice show increased collagen type I and PCPE-1 levels (A,B) and both are co-localized in the diaphragm tissue (C).

(A) Sirius red staining of diaphragm sections from 4 and 8.5 months old mdx mice and age-matched C57/BL/6 (control, wt) (one mouse per group). Red, collagen; Green, non-collagenous proteins. (B) Immunoblot showing increased amounts of collagen type I and PCPE-1 in protein extracts of diaphragms from mdx mice relatively to corresponding wild type (wt) controls (one mouse per group). The amounts of protein applied on each lane were 1 and 25 μg for collagen type I and PCPE-1 detection, respectively. α1 (I), alpha 1 chain of type I collagen. kDa, kilo Dalton. (C) Immunofluorescence analysis for type I collagen and PCPE-1 in diaphragms from a four months old C57/BL/6 (wt) and an aged matched mdx mouse. Collagen type I (red) was detected using Rhodamine-conjugated secondary antibody and PCPE-1 (green) was visualized using a Cy2-conjugated secondary antibody. Light emission was examined at 488 and 580 nm for Rhodamine and Cy2, respectively. Results displayed in each panel are from a representative randomly selected mouse from each group.