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. 1993 Jun;46(6):548–550. doi: 10.1136/jcp.46.6.548

Sequential reactivity of serum against cyst antigens in Toxoplasma infection.

G Woodison 1, A H Balfour 1, J E Smith 1
PMCID: PMC501293  PMID: 8331179

Abstract

AIMS--To compare the recognition of Toxoplasma gondii tachyzoites and cysts by sera, from 10 patients. METHODS--Recognition of antigens from purified tachyzoites (RH strain) and bradyzoites (18691 strain) was compared using western immunoblotting. Sequential serum samples from 10 patients and one laboratory acquired RH infection were used. RESULTS--Recognition of cyst antigens was relatively low and occurred late in infection. The two stages were antigenically distinct with only a few shared bands. CONCLUSION--Immunological recognition of the cystic stage of T gondii is low. This implies that either cysts are poorly immunogenic or that cyst antigen is not available for processing and presentation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cornelissen A. W., Overdulve J. P., Hoenderboom J. M. Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissue by continuous density-gradient centrifugation. Parasitology. 1981 Aug;83(Pt 1):103–108. doi: 10.1017/s0031182000050071. [DOI] [PubMed] [Google Scholar]
  2. Decoster A., Darcy F., Capron A. Recognition of Toxoplasma gondii excreted and secreted antigens by human sera from acquired and congenital toxoplasmosis: identification of markers of acute and chronic infection. Clin Exp Immunol. 1988 Sep;73(3):376–382. [PMC free article] [PubMed] [Google Scholar]
  3. Hunter D., Chadwick P., Balfour A. H., Bridges J. B. Examination of ovine foetal fluid for antibodies to Toxoplasma gondii by the dye test and an indirect immunofluorescence test specific for IgM. Br Vet J. 1982 Jan-Feb;138(1):29–34. doi: 10.1016/s0007-1935(17)31186-7. [DOI] [PubMed] [Google Scholar]
  4. Kasper L. H. Identification of stage-specific antigens of Toxoplasma gondii. Infect Immun. 1989 Mar;57(3):668–672. doi: 10.1128/iai.57.3.668-672.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kasper L. H., Ware P. L. Recognition and characterization of stage-specific oocyst/sporozoite antigens of Toxoplasma gondii by human antisera. J Clin Invest. 1985 May;75(5):1570–1577. doi: 10.1172/JCI111862. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Luft B. J., Brooks R. G., Conley F. K., McCabe R. E., Remington J. S. Toxoplasmic encephalitis in patients with acquired immune deficiency syndrome. JAMA. 1984 Aug 17;252(7):913–917. [PubMed] [Google Scholar]
  8. Lunde M. N., Jacobs L. Antigenic differences between endozoites and cystozoites of Toxoplasma gondii. J Parasitol. 1983 Oct;69(5):806–808. [PubMed] [Google Scholar]
  9. Navia B. A., Petito C. K., Gold J. W., Cho E. S., Jordan B. D., Price R. W. Cerebral toxoplasmosis complicating the acquired immune deficiency syndrome: clinical and neuropathological findings in 27 patients. Ann Neurol. 1986 Mar;19(3):224–238. doi: 10.1002/ana.410190303. [DOI] [PubMed] [Google Scholar]
  10. Potasman I., Araujo F. G., Desmonts G., Remington J. S. Analysis of Toxoplasma gondii antigens recognized by human sera obtained before and after acute infection. J Infect Dis. 1986 Oct;154(4):650–657. doi: 10.1093/infdis/154.4.650. [DOI] [PubMed] [Google Scholar]
  11. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Verhofstede C., Van Gelder P., Rabaey M. The infection-stage-related IgG response to Toxoplasma gondii studied by immunoblotting. Parasitol Res. 1988;74(6):516–520. doi: 10.1007/BF00531628. [DOI] [PubMed] [Google Scholar]
  13. Weiss L. M., Udem S. A., Tanowitz H., Wittner M. Western blot analysis of the antibody response of patients with AIDS and toxoplasma encephalitis: antigenic diversity among Toxoplasma strains. J Infect Dis. 1988 Jan;157(1):7–13. doi: 10.1093/infdis/157.1.7. [DOI] [PubMed] [Google Scholar]
  14. Woodison G., Smith J. E. Identification of the dominant cyst antigens of Toxoplasma gondii. Parasitology. 1990 Jun;100(Pt 3):389–392. doi: 10.1017/s0031182000078665. [DOI] [PubMed] [Google Scholar]

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