Abstract
Gastric biopsy specimens from 283 patients with ulcer and non-ulcer dyspepsia attending five gastroenterology clinics in the northern region of the United Arab Emirates (UAE) were tested by the agar gel test (n = 115) or the ultra-rapid endoscopy room test (n = 168) for the presence of Helicobacter pylori urease. Results were compared with a histological technique using the Romanowsky type (Diff-3) stain for detecting H pylori in both antral and body type gastric mucosa. A sensitivity of 94% and specificity of 100% using the agar gel test compared with 87% sensitivity and 99.3% specificity for the ultra-rapid endoscopy room test. Grading of H pylori in gastric biopsy specimens showed that the higher the histological grade, the more likely that the urease test would be positive. Both forms of urease tests have high specificity for detecting H pylori in gastric biopsy specimens, although the urea agar test has a higher sensitivity than the ultra-rapid test. Low numbers of H pylori in gastric biopsy specimens are the most important determinant of a false negative urease test.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Das S. S., Bain L. A., Karim Q. N., Coelho L. G., Baron J. H. Rapid diagnosis of Campylobacter pyloridis infection. J Clin Pathol. 1987 Jun;40(6):701–702. doi: 10.1136/jcp.40.6.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hazell S. L., Borody T. J., Gal A., Lee A. Campylobacter pyloridis gastritis I: Detection of urease as a marker of bacterial colonization and gastritis. Am J Gastroenterol. 1987 Apr;82(4):292–296. [PubMed] [Google Scholar]
- Marshall B. J., McGechie D. B., Rogers P. A., Glancy R. J. Pyloric Campylobacter infection and gastroduodenal disease. Med J Aust. 1985 Apr 15;142(8):439–444. doi: 10.5694/j.1326-5377.1985.tb113444.x. [DOI] [PubMed] [Google Scholar]
- Marshall B. J., Warren J. R., Francis G. J., Langton S. R., Goodwin C. S., Blincow E. D. Rapid urease test in the management of Campylobacter pyloridis-associated gastritis. Am J Gastroenterol. 1987 Mar;82(3):200–210. [PubMed] [Google Scholar]
- Marshall B. J., Warren J. R., Francis G. J., Langton S. R., Goodwin C. S., Blincow E. D. Rapid urease test in the management of Campylobacter pyloridis-associated gastritis. Am J Gastroenterol. 1987 Mar;82(3):200–210. [PubMed] [Google Scholar]
- McNulty C. A., Dent J. C., Uff J. S., Gear M. W., Wilkinson S. P. Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients. Gut. 1989 Aug;30(8):1058–1062. doi: 10.1136/gut.30.8.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Price A. B. The Sydney System: histological division. J Gastroenterol Hepatol. 1991 May-Jun;6(3):209–222. doi: 10.1111/j.1440-1746.1991.tb01468.x. [DOI] [PubMed] [Google Scholar]
- Schnell G. A., Schubert T. T. Usefulness of culture, histology, and urease testing in the detection of Campylobacter pylori. Am J Gastroenterol. 1989 Feb;84(2):133–137. [PubMed] [Google Scholar]
- Thillainayagam A. V., Arvind A. S., Cook R. S., Harrison I. G., Tabaqchali S., Farthing M. J. Diagnostic efficiency of an ultrarapid endoscopy room test for Helicobacter pylori. Gut. 1991 May;32(5):467–469. doi: 10.1136/gut.32.5.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vaira D., Holton J., Cairns S., Falzon M., Salmon P. Four hour rapid urease test (RUT) for detecting Campylobacter pylori: is it reliable enough to start treatment? J Clin Pathol. 1988 Mar;41(3):355–356. doi: 10.1136/jcp.41.3.355-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaitoun A. M. Use of Romanowsky type (Diff-3) stain for detecting Helicobacter pylori in smears and tissue sections. J Clin Pathol. 1992 May;45(5):448–449. doi: 10.1136/jcp.45.5.448. [DOI] [PMC free article] [PubMed] [Google Scholar]