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. 1999 Jul;52(7):517–520. doi: 10.1136/jcp.52.7.517

Ki-67, oestrogen receptor, and progesterone receptor proteins in the human rete ovarii and in endometriosis.

M S Khan 1, A R Dodson 1, M K Heatley 1
PMCID: PMC501494  PMID: 10605405

Abstract

AIM: To examine proliferative activity using the Ki-67 protein, oestrogen receptor protein, and progesterone receptor protein expression in the rete ovarii, and to make comparisons with their expression in endometriosis. METHODS: Immunohistochemistry was used to study the rete ovarii in 24 cases and endometriosis in seven cases, using antibodies to Ki-67 protein (growth fraction (GF) quantified using a point score method) and oestrogen receptor and progesterone receptor (quantified using the H score method). RESULTS: There was no evidence of a significant difference in the Ki-67 protein, oestrogen receptor, and progesterone receptor in the rete ovarii in different phases of the menstrual cycle (proliferative phase: GF = 1.052, oestrogen receptor H score = 13.4, progesterone receptor H score = 15.32; secretory phase: GF = 0.736, oestrogen receptor H score = 7.5, progesterone receptor H score = 1.84). The expression of all three proteins was greater in the foci of endometriosis (GF = 6.99, oestrogen receptor H score = 152.02, progesterone receptor H score = 127.36) than in the rete ovarii (p < 0.0005-0.0008, Mann-Whitney U test). CONCLUSIONS: There is a low rate of cellular proliferation in the rete ovarii and this structure shows less responsiveness to hormone stimulation than foci of endometriosis. These differences may provide a useful tool to distinguish the rete ovarii from endometriosis in cases of diagnostic difficulty.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amso N. N., Crow J., Shaw R. W. Comparative immunohistochemical study of oestrogen and progesterone receptors in the fallopian tube and uterus at different stages of the menstrual cycle and the menopause. Hum Reprod. 1994 Jun;9(6):1027–1037. doi: 10.1093/oxfordjournals.humrep.a138628. [DOI] [PubMed] [Google Scholar]
  2. Bergqvist A., Fernö M., Skoog L. Quantitative enzyme immunoassay and semiquantitative immunohistochemistry of oestrogen and progesterone receptors in endometriotic tissue and endometrium. J Clin Pathol. 1997 Jun;50(6):496–500. doi: 10.1136/jcp.50.6.496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 23-1986. An 80-year-old woman with anemia, elevation of the erythrocyte sedimentation rate, and a pelvic mass. N Engl J Med. 1986 Jun 12;314(24):1564–1572. doi: 10.1056/NEJM198606123142408. [DOI] [PubMed] [Google Scholar]
  4. Czernobilsky B., Moll R., Levy R., Franke W. W. Co-expression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of the human ovary. Eur J Cell Biol. 1985 May;37:175–190. [PubMed] [Google Scholar]
  5. Gerdes J., Lemke H., Baisch H., Wacker H. H., Schwab U., Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710–1715. [PubMed] [Google Scholar]
  6. Hall P. A., Coates P. J. Assessment of cell proliferation in pathology--what next? Histopathology. 1995 Feb;26(2):105–112. doi: 10.1111/j.1365-2559.1995.tb00639.x. [DOI] [PubMed] [Google Scholar]
  7. Heatley M., Whiteside C., Maxwell P., Toner P. G. Correspondence Re: WM Hanna, Kahn HJ, and J-AW Chapman. The correlation of Ki-67 growth factor and ERICA in breast cancer. Mod Pathol 5:220, 1992. Mod Pathol. 1994 Oct;7(8):889–890. [PubMed] [Google Scholar]
  8. McCarty K. S., Jr, Miller L. S., Cox E. B., Konrath J., McCarty K. S., Sr Estrogen receptor analyses. Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies. Arch Pathol Lab Med. 1985 Aug;109(8):716–721. [PubMed] [Google Scholar]
  9. Nisolle M., Casanas-Roux F., Donnez J. Immunohistochemical analysis of proliferative activity and steroid receptor expression in peritoneal and ovarian endometriosis. Fertil Steril. 1997 Nov;68(5):912–919. doi: 10.1016/s0015-0282(97)00341-5. [DOI] [PubMed] [Google Scholar]
  10. Nogales F. F., Carvia R. E., Donné C., Campello T. R., Vidal M., Martin A. Adenomas of the rete ovarii. Hum Pathol. 1997 Dec;28(12):1428–1433. doi: 10.1016/s0046-8177(97)90235-5. [DOI] [PubMed] [Google Scholar]
  11. Park S. H., Kim I. Histogenetic consideration of ovarian sex cord-stromal tumors analyzed by expression pattern of cytokeratins, vimentin, and laminin. Correlation studies with human gonads. Pathol Res Pract. 1994 May;190(5):449–456. doi: 10.1016/S0344-0338(11)80207-4. [DOI] [PubMed] [Google Scholar]
  12. Zidan A., Christie Brown J. S., Peston D., Shousha S. Oestrogen and progesterone receptor assessment in core biopsy specimens of breast carcinoma. J Clin Pathol. 1997 Jan;50(1):27–29. doi: 10.1136/jcp.50.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. van den Hurk R., Dijkstra G., van Mil F. N., Hulshof S. C., van den Ingh T. S. Distribution of the intermediate filament proteins vimentin, keratin, and desmin in the bovine ovary. Mol Reprod Dev. 1995 Aug;41(4):459–467. doi: 10.1002/mrd.1080410408. [DOI] [PubMed] [Google Scholar]

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